GapMind for catabolism of small carbon sources

 

L-phenylalanine catabolism in Brucella inopinata BO1

Best path

livF, livG, livH, livM, livJ, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, aacS, atoB

Rules

Overview: Phenylalanine utilization in GapMind is based on MetaCyc pathway L-phenylalanine degradation I (aerobic, via tyrosine, link), pathway II (anaerobic, via phenylacetaldehyde dehydrogenase, link), degradation via phenylpyruvate:ferredoxin oxidoreductase (PMC3346364), or degradation via phenylacetaldehyde:ferredoxin oxidoreductase (PMID:24214948). (MetaCyc describes additional pathways, but they do not result in carbon incorporation or are not reported in prokaryotes, so they are not included in GapMind.)

76 steps (35 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
livF L-phenylalanine ABC transporter, ATPase component 1 (LivF) BIBO1_RS12235 BIBO1_RS14990
livG L-phenylalanine ABC transporter, ATPase component 2 (LivG) BIBO1_RS12240 BIBO1_RS19765
livH L-phenylalanine ABC transporter, permease component 1 (LivH) BIBO1_RS12250 BIBO1_RS14975
livM L-phenylalanine ABC transporter, permease component 2 (LivM) BIBO1_RS12245
livJ L-phenylalanine ABC transporter, substrate-binding component LivJ/LivK BIBO1_RS12225 BIBO1_RS12220
PAH phenylalanine 4-monooxygenase
PCBD pterin-4-alpha-carbinoalamine dehydratase
QDPR 6,7-dihydropteridine reductase
HPD 4-hydroxyphenylpyruvate dioxygenase
hmgA homogentisate dioxygenase
maiA maleylacetoacetate isomerase BIBO1_RS08965 BIBO1_RS10995
fahA fumarylacetoacetate hydrolase BIBO1_RS12805
aacS acetoacetyl-CoA synthetase BIBO1_RS06845 BIBO1_RS13010
atoB acetyl-CoA C-acetyltransferase BIBO1_RS17190 BIBO1_RS16830
Alternative steps:
ARO10 phenylpyruvate decarboxylase
ARO8 L-phenylalanine transaminase BIBO1_RS11000 BIBO1_RS11810
aroP L-phenylalanine:H+ symporter AroP BIBO1_RS14845
atoA acetoacetyl-CoA transferase, A subunit BIBO1_RS16820
atoD acetoacetyl-CoA transferase, B subunit BIBO1_RS16825
badH 2-hydroxy-cyclohexanecarboxyl-CoA dehydrogenase BIBO1_RS19255 BIBO1_RS08590
badI 2-ketocyclohexanecarboxyl-CoA hydrolase BIBO1_RS06755 BIBO1_RS15870
badK cyclohex-1-ene-1-carboxyl-CoA hydratase BIBO1_RS06755 BIBO1_RS15870
bamB class II benzoyl-CoA reductase, BamB subunit
bamC class II benzoyl-CoA reductase, BamC subunit
bamD class II benzoyl-CoA reductase, BamD subunit
bamE class II benzoyl-CoA reductase, BamE subunit
bamF class II benzoyl-CoA reductase, BamF subunit
bamG class II benzoyl-CoA reductase, BamG subunit
bamH class II benzoyl-CoA reductase, BamH subunit BIBO1_RS07440
bamI class II benzoyl-CoA reductase, BamI subunit
bcrA ATP-dependent benzoyl-CoA reductase, alpha subunit
bcrB ATP-dependent benzoyl-CoA reductase, beta subunit
bcrC ATP-dependent benzoyl-CoA reductase, gamma subunit
bcrD ATP-dependent benzoyl-CoA reductase, delta subunit
boxA benzoyl-CoA epoxidase, subunit A
boxB benzoyl-CoA epoxidase, subunit B
boxC 2,3-epoxybenzoyl-CoA dihydrolase
boxD 3,4-dehydroadipyl-CoA semialdehyde dehydrogenase
Ch1CoA cyclohex-1-ene-1-carbonyl-CoA dehydrogenase BIBO1_RS17185 BIBO1_RS06840
dch cyclohexa-1,5-diene-1-carboxyl-CoA hydratase BIBO1_RS06755 BIBO1_RS15870
ech (S)-3-hydroxybutanoyl-CoA hydro-lyase BIBO1_RS06755 BIBO1_RS15870
fadB (S)-3-hydroxybutanoyl-CoA dehydrogenase BIBO1_RS18180 BIBO1_RS05845
gcdH glutaryl-CoA dehydrogenase BIBO1_RS08430 BIBO1_RS17185
had 6-hydroxycyclohex-1-ene-1-carbonyl-CoA dehydrogenase
iorA phenylpyruvate:ferredoxin oxidoreductase, IorA subunit
iorAB phenylpyruvate:ferredoxin oxidoreductase, fused IorA/IorB
iorB phenylpyruvate:ferredoxin oxidoreductase, IorB subunit
oah 6-oxocyclohex-1-ene-1-carbonyl-CoA hydratase
paaA phenylacetyl-CoA 1,2-epoxidase, subunit A
paaB phenylacetyl-CoA 1,2-epoxidase, subunit B
paaC phenylacetyl-CoA 1,2-epoxidase, subunit C
paaE phenylacetyl-CoA 1,2-epoxidase, subunit E
paaF 2,3-dehydroadipyl-CoA hydratase BIBO1_RS15870 BIBO1_RS06755
paaG 1,2-epoxyphenylacetyl-CoA isomerase / 2-(oxepinyl)acetyl-CoA isomerase / didehydroadipyl-CoA isomerase BIBO1_RS06755 BIBO1_RS15870
paaH 3-hydroxyadipyl-CoA dehydrogenase BIBO1_RS18180 BIBO1_RS15875
paaJ1 3-oxo-5,6-dehydrosuberyl-CoA thiolase BIBO1_RS16830 BIBO1_RS18185
paaJ2 3-oxoadipyl-CoA thiolase BIBO1_RS16830 BIBO1_RS18185
paaK phenylacetate-CoA ligase BIBO1_RS13010
paaZ1 oxepin-CoA hydrolase BIBO1_RS06755 BIBO1_RS15870
paaZ2 3-oxo-5,6-didehydrosuberyl-CoA semialdehyde dehydrogenase
pad-dh phenylacetaldehyde dehydrogenase BIBO1_RS15370 BIBO1_RS10835
padB phenylacetyl-CoA dehydrogenase, PadB subunit
padC phenylacetyl-CoA dehydrogenase, PadC subunit
padD phenylacetyl-CoA dehydrogenase, PadD subunit
padE phenylglyoxylate dehydrogenase, gamma subunit
padF phenylglyoxylate dehydrogenase, delta subunit
padG phenylglyoxylate dehydrogenase, alpha subunit
padH phenylglyoxylate dehydrogenase, epsilon subunit
padI phenylglyoxylate dehydrogenase, beta subunit
pfor phenylacetaldeyde:ferredoxin oxidoreductase
pimB 3-oxopimeloyl-CoA:CoA acetyltransferase BIBO1_RS16830 BIBO1_RS18185
pimC pimeloyl-CoA dehydrogenase, small subunit
pimD pimeloyl-CoA dehydrogenase, large subunit BIBO1_RS18240
pimF 6-carboxyhex-2-enoyl-CoA hydratase BIBO1_RS18180
PPDCalpha phenylpyruvate decarboxylase, alpha subunit BIBO1_RS17495
PPDCbeta phenylpyruvate decarboxylase, beta subunit BIBO1_RS17500 BIBO1_RS08620

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory