catabolism of small carbon sources in Thermophagus xiamenensis HS1

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Thermophagus xiamenensis HS1

Pathways are sorted by completeness. Sort by name instead.

Pathway	Steps
galactose	sglS, galK, galT, galE, pgmA
arabinose	Echvi_1880, araA, araB, araD
maltose	malI, malP, pgmB, glk
trehalose	BT2158, klh, SSS-glucose, glk
cellobiose	bgl, SSS-glucose, glk
xylose	xylT, xylA, xylB
glucose	SSS-glucose, glk
alanine	alsT
asparagine	ans, glt
fructose	BT1758, scrK
aspartate	glt
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
threonine	tdcC, tdh, kbl, gcvP, gcvT, gcvH, lpd
rhamnose	rhaT, rhaM, rhaA, rhaB, rhaD, tpi, aldA
glucuronate	dctP, dctQ, dctM, uxaC, uxuB, uxuA, kdgK, eda
arginine	rocE, arcA, arcB, arcC, rocD, rocA
acetate	actP, ackA, pta
citrate	SLC13A5, acn, icd
sucrose	ams, SSS-glucose, glk
deoxyinosine	nupC, deoD, deoB, deoC, adh, ackA, pta
propionate	putP, prpE, pccA, pccB, epi, mcm-large, mcm-small
ethanol	etoh-dh-nad, adh, ackA, pta
NAG	nagP, nagK, nagA, nagB
glucosamine	nagX, nagP, nagK, nagA, nagB
glutamate	gltS, gdhA
D-lactate	lctP, D-LDH
mannitol	mtlA, mtlD
mannose	STP6, mannokinase, manA
proline	proY, put1, putA
fumarate	dctA
glucose-6-P	uhpT
L-malate	sdlC
2-oxoglutarate	kgtP
pyruvate	SLC5A8
succinate	sdc
L-lactate	lctP, L-LDH
D-serine	cycA, dsdA
serine	serP, sdaB
sorbitol	mtlA, srlD
isoleucine	Bap2, vorA *, vorB, vorC, acdH, ech, ivdG, fadA, pccA, pccB, epi, mcm-large, mcm-small
leucine	leuT, ilvE, vorA *, vorB, vorC, liuA, liuB, liuD, liuC, liuE, aacS, atoB
deoxyribose	deoP, deoK, deoC, adh, ackA, pta
galacturonate	exuT, uxaC, uxaB, uxaA, kdgK, eda
gluconate	gntT, gntK, gnd
xylitol	PLT5, xdhA, xylB
fucose	fucP, fucU, fucI, fucK, fucA, tpi, aldA
thymidine	nupC, deoA, deoB, deoC, adh, ackA, pta
glycerol	glpF, dhaD, dhaK', tpi
D-alanine	cycA, dadA
ribose	rbsU, rbsK
tryptophan	aroP, tnaA
valine	Bap2, vorA *, vorB, vorC, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcm-large, mcm-small
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, rocD, rocA
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, aacS, atoB
histidine	permease, hutH, hutU, hutI, hutG
putrescine	puuP, patA, patD, gabT, gabD
lysine	lysP, lat, amaB, lysN, hglS, ydiJ
myoinositol	iolT, iolG, iolM, iolN, iolO, uxaE, uxuB, uxuA, kdgK, eda
tyrosine	aroP, HPD, hmgA, maiA, fahA, aacS, atoB
4-hydroxybenzoate	pcaK, pobA, praA, xylF, mhpD, mhpE, adh, ackA, pta
phenylalanine	aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, aacS, atoB
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources