catabolism of small carbon sources in Serinicoccus profundi MCCC 1A05965

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Serinicoccus profundi MCCC 1A05965

Pathways are sorted by completeness. Sort by name instead.

Pathway	Steps
citrate	tctA, tctB, tctC, acn, icd
glutamate	gltL, gluB, gluC, gluD, gdhA
cellobiose	bgl, MFS-glucose, glk
maltose	susB, MFS-glucose, glk
sucrose	ams, MFS-glucose, glk
glucose	MFS-glucose, glk
fumarate	sdcL
L-malate	sdlC
succinate	sdc
propionate	lctP, prpE, pccA, pccB, epi, mcm-large, mcm-small
ethanol	etoh-dh-nad, adh, ackA, pta
glycerol	glpF, glpK, glpD, tpi
D-lactate	lctP, glcD, glcE, glcF
acetate	actP, ackA, pta
gluconate	gntT, gntK, gnd
proline	ectP, put1, putA
trehalose	treF, MFS-glucose, glk
ribose	rbsA, rbsB, rbsC, rbsK
asparagine	ans, glt
fructose	frcA, frcB, frcC, scrK
alanine	alsT
aspartate	glt
2-oxoglutarate	kgtP
pyruvate	yjcH, actP
isoleucine	natA, natB, natC, natD, natE, bkdA, bkdB, bkdC, lpd, acdH, ech, ivdG, fadA, pccA, pccB, epi, mcm-large, mcm-small
threonine	braC, braD, braE, braF, braG, ltaE, adh, ackA, pta, gcvP, gcvT, gcvH, lpd
leucine	natA, natB, natC, natD, natE, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, atoA, atoD, atoB
phenylalanine	aroP, ARO8, PPDCalpha, PPDCbeta, pad-dh, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2
arginine	rocE, arcA, arcB, arcC, rocD, PRO3, put1, putA
deoxyinosine	nupC, deoD, deoB, deoC, adh, ackA, pta
thymidine	nupC, deoA, deoB, deoC, adh, ackA, pta
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2
deoxyribose	deoP, deoK, deoC, adh, ackA, pta
histidine	natA, natB, natC, natD, natE, hutH, hutU, hutI, hutF, hutG'
L-lactate	lctP, lutA, lutB, lutC
tyrosine	aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
putrescine	puuP, patA, patD, gabT, gabD
serine	braC, braD, braE, braF, braG, sdaB
valine	natA, natB, natC, natD, natE, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcm-large, mcm-small
NAG	nagEcba, nagA, nagB
galactose	galP, galK, galT, galE, pgmA
glucosamine	gamP, nagB
mannose	manP, manA
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, rocD, PRO3, put1, putA
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
glucose-6-P	uhpT
D-serine	cycA, dsdA
sorbitol	SOT, sdh, scrK
xylose	xylT, xylA, xylB
lysine	lysP, davB, davA, davT, davD, gcdG, gcdH, ech, fadB, atoB
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, atoA, atoD, atoB
mannitol	PLT5, mt1d, mak, manA
D-alanine	cycA, dadA
tryptophan	aroP, tnaA
xylitol	fruI, x5p-reductase
fucose	fucP, fucU, fucI, fucK, fucA, tpi, aldA
arabinose	araE, araA, araB, araD
glucuronate	exuT, udh, gci, kdgD, dopDH
4-hydroxybenzoate	pcaK, pobA, praA, xylF, mhpD, mhpE, adh, ackA, pta
galacturonate	exuT, udh, uxuL, garD, kdgD, dopDH
rhamnose	rhaT, LRA1, LRA2, LRA3, LRA5, LRA6
myoinositol	SMIT1, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA, tpi

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources