L-histidine catabolism in Rhizobium freirei PRF 81

Best path

braC, braD, braE, braF, braG, hutH, hutU, hutI, hutF, hutG'

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

• all:
  • histidine-transport, hutH, hutU, hutI and hutG
  • or histidine-transport, hutH, hutU, hutI, hutH, hutF and hutG'
  • Comment: In pathway I, hutH deaminates histidine to urocanate, hutU hydrates it to 4-imidazolone-5-propanoate, hutI hydrolyzes it to N-formiminoglutamate, and hutG hydrolyzes it to formamide and glutamate. Formamide can be cleaved to formate and ammonia, but it could also be secreted, so this is not described. Similarly, formate may be oxidized to CO2 or secreted. Pathway II also involves conversion to N-formiminoglutamate, but then it is deaminated to N-formylglutamate by hutF, and hydrolyzed to formate and glutamate by hutG'.
• histidine-transport:
  • hisP, hisM, hisQ and hisJ
  • or PA5503, PA5504 and PA5505
  • or Ac3H11_2562, Ac3H11_2561, Ac3H11_2560, Ac3H11_2555 and Ac3H11_2554
  • or natA, natB, natC, natD and natE
  • or aapJ, aapQ, aapM and aapP
  • or braC, braD, braE, braF and braG
  • or hutV, hutW and hutX
  • or bgtA and bgtB
  • or BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010 and BPHYT_RS24015
  • or permease
  • or Ga0059261_1577
  • or S15A3
  • or LAT2
  • or LHT
  • or SLC38A3
  • or PTR2
  • Comment: Transporters were identified using query: transporter:histidine:L-histidine:his

48 steps (37 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
braC	ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC	RHSP_RS00440	RHSP_RS24770
braD	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD)	RHSP_RS00465	RHSP_RS18045
braE	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE)	RHSP_RS00460	RHSP_RS17305
braF	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF)	RHSP_RS00455	RHSP_RS18030
braG	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG)	RHSP_RS00450	RHSP_RS18040
hutH	histidine ammonia-lyase	RHSP_RS27975	RHSP_RS24410
hutU	urocanase	RHSP_RS27970	RHSP_RS02670
hutI	imidazole-5-propionate hydrolase	RHSP_RS27980
hutF	N-formiminoglutamate deiminase	RHSP_RS27985
hutG'	N-formylglutamate amidohydrolase	RHSP_RS13170
Alternative steps:
aapJ	L-histidine ABC transporter, substrate-binding component AapJ	RHSP_RS15285	RHSP_RS00995
aapM	L-histidine ABC transporter, permease component 2 (AapM)	RHSP_RS15275	RHSP_RS00985
aapP	L-histidine ABC transporter, ATPase component AapP	RHSP_RS15270	RHSP_RS08130
aapQ	L-histidine ABC transporter, permease component 1 (AapQ)	RHSP_RS15280	RHSP_RS00990
Ac3H11_2554	ABC transporter for L-Histidine, permease component 2	RHSP_RS28425	RHSP_RS01035
Ac3H11_2555	L-histidine ABC transporter, substrate-binding component 2	RHSP_RS07705	RHSP_RS28420
Ac3H11_2560	L-histidine ABC transporter, ATPase component	RHSP_RS00350	RHSP_RS08495
Ac3H11_2561	L-histidine ABC transporter, permease component 1	RHSP_RS08485	RHSP_RS27530
Ac3H11_2562	L-histidine ABC transporter, substrate-binding component 1
bgtA	L-histidine ABC transporter, ATPase component BgtA	RHSP_RS24640	RHSP_RS08130
bgtB	L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
BPHYT_RS24000	L-histidine ABC transporter, substrate-binding component	RHSP_RS24390	RHSP_RS22955
BPHYT_RS24005	L-histidine ABC transporter, permease component 1	RHSP_RS24395	RHSP_RS08830
BPHYT_RS24010	L-histidine ABC transporter, permease component 2	RHSP_RS24400	RHSP_RS22960
BPHYT_RS24015	L-histidine ABC transporter, ATPase component	RHSP_RS24405	RHSP_RS00540
Ga0059261_1577	L-histidine transporter
hisJ	L-histidine ABC transporter, substrate-binding component HisJ	RHSP_RS03670	RHSP_RS08825
hisM	L-histidine ABC transporter, permease component 1 (HisM)	RHSP_RS22960	RHSP_RS24400
hisP	L-histidine ABC transporter, ATPase component HisP	RHSP_RS00540	RHSP_RS08840
hisQ	L-histidine ABC transporter, permease component 2 (HisQ)	RHSP_RS22965	RHSP_RS24395
hutG	N-formiminoglutamate formiminohydrolase
hutV	L-histidine ABC transporter, ATPase component HutV	RHSP_RS09695	RHSP_RS27785
hutW	L-histidine ABC transporter, permease component HutW	RHSP_RS09690	RHSP_RS11545
hutX	L-histidine ABC transporter, substrate-binding component HutX	RHSP_RS09680	RHSP_RS24955
LAT2	L-histidine transporter
LHT	L-histidine transporter
natA	L-histidine ABC transporter, ATPase component 1 (NatA)	RHSP_RS09470	RHSP_RS00455
natB	L-histidine ABC transporter, substrate-binding component NatB
natC	L-histidine ABC transporter, permease component 1 (NatC)
natD	L-histidine ABC transporter, permease component 2 (NatD)	RHSP_RS09460	RHSP_RS22335
natE	L-histidine ABC transporter, ATPase component 2 (NatE)	RHSP_RS09465	RHSP_RS00450
PA5503	L-histidine ABC transporter, ATPase component	RHSP_RS12430	RHSP_RS07695
PA5504	L-histidine ABC transporter, permease component	RHSP_RS12435
PA5505	L-histidine ABC transporter, substrate-binding component	RHSP_RS12425
permease	L-histidine permease	RHSP_RS26460
PTR2	L-histidine:H+ symporter
S15A3	L-histidine transporter
SLC38A3	L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.