Finding step potA for putrescine catabolism in Weissella oryzae SG25
5 candidates for potA: putrescine ABC transporter, ATPase component (PotA/PotG)
| Score | Gene | Description | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| hi | TT13_RS07710 | ABC transporter ATP-binding protein | Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD (characterized) | 67% | 96% | 494.2 | Fe(3+) ions import ATP-binding protein FbpC, component of Hexose-phosphate transporter | 39% | 256.5 |
| lo | TT13_RS02220 | glycine betaine/L-proline ABC transporter ATP-binding protein | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) | 33% | 81% | 177.9 | BusAA, component of Uptake system for glycine-betaine (high affinity) and proline (low affinity) (OpuAA-OpuABC) or BusAA-ABC of Lactococcus lactis). BusAA, the ATPase subunit, has a C-terminal tandem cystathionine β-synthase (CBS) domain which is the cytoplasmic K+ sensor for osmotic stress (osmotic strength)while the BusABC subunit has the membrane and receptor domains fused to each other (Biemans-Oldehinkel et al., 2006; Mahmood et al., 2006; Gul et al. 2012). An N-terminal amphipathic α-helix of OpuA is necessary for high activity but is not critical for biogenesis or the ionic regulation of transport | 74% | 574.7 |
| lo | TT13_RS07590 | amino acid ABC transporter ATP-binding protein | spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31 (characterized) | 35% | 63% | 154.8 | Glutamine ABC transporter ATP-binding protein, component of Glutamine transporter, GlnQP. Takes up glutamine, asparagine and glutamate which compete for each other for binding both substrate and the transmembrane protein constituent of the system (Fulyani et al. 2015). Tandem substrate binding domains (SBDs) differ in substrate specificity and affinity, allowing cells to efficiently accumulate different amino acids via a single ABC transporter. Analysis revealed the roles of individual residues in determining the substrate affinity | 66% | 314.3 |
| lo | TT13_RS00210 | methionine ABC transporter ATP-binding protein | PotG aka B0855, component of Putrescine porter (characterized) | 30% | 72% | 150.2 | AtmD (MetN), component of The methionine porter, AtmBDE | 56% | 379.4 |
| lo | TT13_RS01035 | amino acid ABC transporter ATP-binding protein | PotG aka B0855, component of Putrescine porter (characterized) | 37% | 60% | 146.7 | Arginine transport ATP-binding protein ArtM | 48% | 221.5 |
Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.
GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.
Definition of step potA
- Curated sequence P31134: PotG aka B0855, component of Putrescine porter. putrescine ABC transporter ATP binding subunit (EC 7.6.2.16). putrescine ABC transporter ATP binding subunit (EC 7.6.2.16)
- Curated sequence CH_024626: spermidine/putrescine ABC transporter, ATP-binding protein PotA; EC 3.6.3.31. Spermidine/putrescine import ATP-binding protein PotA; EC 7.6.2.11. Spermidine/putrescine import ATP-binding protein PotA aka B1126, component of Polyamine (putrescine/spermidine) uptake porter. spermidine preferential ABC transporter ATP binding subunit (EC 7.6.2.11; EC 7.6.2.16). spermidine preferential ABC transporter ATP binding subunit (EC 7.6.2.11; EC 7.6.2.16)
- Curated sequence Q97Q42: Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD
- Comment: 4-part ABC transporters include E. coli potABCD or potGHIF, which are related to each other, and a related system, also named potABCD, from Streptococcus pneuomoniae.
Or cluster all characterized potA proteins
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory