catabolism of small carbon sources in Bryobacter aggregatus MPL3

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Bryobacter aggregatus MPL3

Pathways are sorted by completeness. Sort by name instead.

Pathway	Steps
deoxyinosine	nupC, deoD, deoB, deoC, adh, acs
thymidine	nupC, deoA, deoB, deoC, adh, acs
ethanol	etoh-dh-nad, adh, acs
L-lactate	lctP, lctO, acs
glutamate	gltP, gdhA
D-lactate	lctP, D-LDH
aspartate	glt
propionate	lctP, prpE, pccA, pccB, epi, mcm-large, mcm-small
proline	CCNA_00435, put1, putA
glycerol	glpF, glpK, glpD, tpi
acetate	dctA, acs
asparagine	ans, glt
xylose	xylF_Tm, xylE_Tm, xylK_Tm, xylA, xylB
fumarate	dctA
L-malate	dctA
pyruvate	btsT
succinate	dctA
threonine	tdcC, ltaE, adh, acs, gcvP, gcvT, gcvH, lpd
arginine	rocE, rocF, rocD, PRO3, put1, putA
glucuronate	exuT, uxaC, uxuB, uxuA, kdgK, eda
deoxyribose	deoP, deoK, deoC, adh, acs
citrate	SLC13A5, acn, icd
gluconate	gntT, gntK, gnd
isoleucine	Bap2, bkdA, bkdB, bkdC, lpd, acdH, ech, ivdG, fadA, pccA, pccB, epi, mcm-large, mcm-small
galactose	galP, galK, galT, galE, pgmA
sucrose	sut, SUS, scrK, galU, pgmA
fructose	Slc2a5, scrK
serine	serP, sdaB
galacturonate	exuT, uxaC, uxaB, uxaA, kdgK, eda
mannitol	PLT5, mt2d, scrK
mannose	manMFS, man-isomerase, scrK
sorbitol	SOT, sdh, scrK
xylitol	PLT5, xdhA, xylB
glucosamine	nagX, nagEcba, nagA, nagB
ribose	rbsA, rbsB, rbsC, rbsK
putrescine	potA, potB, potC, potD, patA, patD, gabT, gabD
alanine	cycA
glucose	ptsG-crr
glucose-6-P	uhpT
2-oxoglutarate	kgtP
lactose	lacA', lacC', lacB', klh, ptsG-crr
D-serine	cycA, dsdA
NAG	nagEcba, nagA, nagB
rhamnose	rhaT, LRA1, LRA2, LRA3, LRA5, LRA6
cellobiose	cdt, cbp, pgmA, glk
trehalose	TRET1, PsTP, pgmA, glk
valine	Bap2, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcm-large, mcm-small
D-alanine	cycA, dadA
maltose	susB, ptsG-crr
tryptophan	aroP, tnaA
arabinose	araE, xacB, xacC, xacD, xacE, xacF
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, aacS, atoB
lysine	lysP, lat, amaB, lysN, hglS, ydiJ
leucine	leuT, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, aacS, atoB
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, rocD, PRO3, put1, putA
fucose	HSERO_RS05250, HSERO_RS05255, HSERO_RS05260, fucU, fdh, fuconolactonase, fucD, fucDH, KDF-hydrolase
histidine	PA5503, PA5504, PA5505, hutH, hutU, hutI, hutG
4-hydroxybenzoate	pcaK, pobA, praA, xylF, mhpD, mhpE, adh, acs
tyrosine	aroP, HPD, hmgA, maiA, fahA, aacS, atoB
myoinositol	iolT, iolG, iolM, iolN, iolO, uxaE, uxuB, uxuA, kdgK, eda
phenylalanine	aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, aacS, atoB
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources