L-arabinose catabolism in Alicyclobacillus ferrooxydans TC-34

GapMind for catabolism of small carbon sources

L-arabinose catabolism in Alicyclobacillus ferrooxydans TC-34

Best path

Rules

Overview: L-arabinose utilization in GapMind is based on MetaCyc pathways L-arabinose degradation I, via xylulose 5-phosphate (link); III, oxidation to 2-oxoglutarate (link); and IV, via glycolaldehyde (link). Pathway II via xylitol and xylulose is not represented in GapMind because it is not reported in prokaryotes (link).

glycolaldehyde-dehydrogenase:

aldA
or aldox-large, aldox-med and aldox-small

all:

arabinose-transport, araA, araB and araD
or arabinose-transport, xacB, xacC, xacD, xacE and xacF
or arabinose-transport, xacB, xacC, xacD, KDG-aldolase, glycolaldehyde-dehydrogenase, gyaR and glcB
Comment: In pathway I, isomerase araA forms L-ribulose, kinase araB forms ribulose 5-phosphate, and epimerase araD forms D-xylulose 5-phosphate, which is an intermediate in the pentose phosphate pathway. In pathway III, the 1-dehydrogenase xacB (which acts on the furanose form, not the usual pyranose form?) forms arabino-1,4-lactone, lactonase xacC forms arbinonate, two dehydratases form 2-dehydro-3-deoxy-L-arabinonate and 2,5-dioxopentanonate (α-ketoglutarate semialdehyde), and dehydrogenase xacF forms 2-oxoglutarate, which is an intermediate in the TCA cycle. (Fitness data suggests that L-arabinose 1-epimerase or mutarotase is also involved, perhaps in creating the correct epimer for the 1-dehydrogenase, but is not included in GapMind.) Pathway IV begins as in pathway III, to 2-dehydro-3-deoxy-L-arabinonate, followed by KDG aldolase to pyruvate and glycolaldehyde; the glycolaldehyde is oxidized to glycolate and then to glyoxylate, and combined with acetyl-CoA by malate synthase, which is a TCA cycle intermediate. (Other pathways for glyxoylate assimilation are known but are not represented here.)

arabinose-transport:

gguA, gguB and chvE
or araF, araG and araH
or araS, araT, araU and araV
or xacG, xacH, xacI, xacJ and xacK
or xylFsa, xylGsa and xylHsa
or araUsh, araVsh, araWsh and araZsh
or araE
or BT0355
or Echvi_1880
Comment: Transporters were identified using query: transporter:arabinose:L-arabinose:L-arabinofuranose:L-arabinopyranose:beta-L-arabinose:CPD-12045:CPD-12046

40 steps (24 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
araE	L-arabinose:H+ symporter	AN477_RS09520	AN477_RS14365
araA	L-arabinose isomerase	AN477_RS09510
araB	ribulokinase	AN477_RS09525
araD	L-ribulose-5-phosphate epimerase	AN477_RS09515
Alternative steps:
aldA	(glycol)aldehyde dehydrogenase	AN477_RS17560	AN477_RS23050
aldox-large	(glycol)aldehyde oxidoreductase, large subunit	AN477_RS20815	AN477_RS15490
aldox-med	(glycol)aldehyde oxidoreductase, medium subunit	AN477_RS15480	AN477_RS20810
aldox-small	(glycol)aldehyde oxidoreductase, small subunit	AN477_RS20820	AN477_RS15485
araF	L-arabinose ABC transporter, substrate-binding component AraF
araG	L-arabinose ABC transporter, ATPase component AraG	AN477_RS02610
araH	L-arabinose ABC transporter, permease component AraH
araS	L-arabinose ABC transporter, substrate-binding component AraS
araT	L-arabinose ABC transporter, permease component 1 (AraT)	AN477_RS07330
araU	L-arabinose ABC transporter, permease component 2 (AraU)
araUsh	L-arabinose ABC transporter, substrate-binding component AraU(Sh)
araV	L-arabinose ABC transporter, ATPase component AraV	AN477_RS00250	AN477_RS09235
araVsh	L-arabinose ABC transporter, ATPase component AraV(Sh)	AN477_RS02610	AN477_RS01360
araWsh	L-arabinose ABC transporter, permease component 1 AraW(Sh)
araZsh	L-arabinose ABC transporter, permease component 2 AraZ(Sh)
BT0355	L-arabinose:Na+ symporter
chvE	L-arabinose ABC transporter, substrate-binding component ChvE
Echvi_1880	L-arabinose:Na+ symporter
gguA	L-arabinose ABC transporter, ATPase component GguA	AN477_RS02610
gguB	L-arabinose ABC transporter, permease component GguB
glcB	malate synthase	AN477_RS12645	AN477_RS00875
gyaR	glyoxylate reductase	AN477_RS12745	AN477_RS21150
KDG-aldolase	2-dehydro-3-deoxy-L-arabinonate aldolase
xacB	L-arabinose 1-dehydrogenase	AN477_RS09880	AN477_RS06355
xacC	L-arabinono-1,4-lactonase
xacD	L-arabinonate dehydratase	AN477_RS21080	AN477_RS21095
xacE	2-dehydro-3-deoxy-L-arabinonate dehydratase	AN477_RS00820
xacF	alpha-ketoglutarate semialdehyde dehydrogenase	AN477_RS21120	AN477_RS10495
xacG	L-arabinose ABC transporter, substrate-binding component XacG
xacH	L-arabinose ABC transporter, permease component 1 (XacH)	AN477_RS07470	AN477_RS04360
xacI	L-arabinose ABC transporter, permease component 2 (XacI)	AN477_RS07475
xacJ	L-arabinose ABC transporter, ATPase component 1 (XacJ)	AN477_RS00250	AN477_RS09235
xacK	L-arabinose ABC transporter, ATPase component 2 (XacK)	AN477_RS00250	AN477_RS09235
xylFsa	L-arabinose ABC transporter, substrate-binding component XylF
xylGsa	L-arabinose ABC transporter, ATPase component XylG	AN477_RS02610	AN477_RS15705
xylHsa	L-arabinose ABC transporter, permease component XylH

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources