L-histidine catabolism in Pantoea rwandensis LMG 26275

Best path

BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010, BPHYT_RS24015, hutH, hutU, hutI, hutF, hutG'

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

• all:
  • histidine-transport, hutH, hutU, hutI and hutG
  • or histidine-transport, hutH, hutU, hutI, hutH, hutF and hutG'
  • Comment: In pathway I, hutH deaminates histidine to urocanate, hutU hydrates it to 4-imidazolone-5-propanoate, hutI hydrolyzes it to N-formiminoglutamate, and hutG hydrolyzes it to formamide and glutamate. Formamide can be cleaved to formate and ammonia, but it could also be secreted, so this is not described. Similarly, formate may be oxidized to CO2 or secreted. Pathway II also involves conversion to N-formiminoglutamate, but then it is deaminated to N-formylglutamate by hutF, and hydrolyzed to formate and glutamate by hutG'.
• histidine-transport:
  • hisP, hisM, hisQ and hisJ
  • or PA5503, PA5504 and PA5505
  • or Ac3H11_2562, Ac3H11_2561, Ac3H11_2560, Ac3H11_2555 and Ac3H11_2554
  • or natA, natB, natC, natD and natE
  • or aapJ, aapQ, aapM and aapP
  • or braC, braD, braE, braF and braG
  • or hutV, hutW and hutX
  • or bgtA and bgtB
  • or BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010 and BPHYT_RS24015
  • or permease
  • or Ga0059261_1577
  • or S15A3
  • or LAT2
  • or LHT
  • or SLC38A3
  • or PTR2
  • Comment: Transporters were identified using query: transporter:histidine:L-histidine:his

48 steps (36 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
BPHYT_RS24000	L-histidine ABC transporter, substrate-binding component	HA51_RS25195	HA51_RS07740
BPHYT_RS24005	L-histidine ABC transporter, permease component 1	HA51_RS25190	HA51_RS07735
BPHYT_RS24010	L-histidine ABC transporter, permease component 2	HA51_RS25185	HA51_RS07730
BPHYT_RS24015	L-histidine ABC transporter, ATPase component	HA51_RS25180	HA51_RS11195
hutH	histidine ammonia-lyase	HA51_RS18455	HA51_RS25175
hutU	urocanase	HA51_RS18450
hutI	imidazole-5-propionate hydrolase	HA51_RS18475
hutF	N-formiminoglutamate deiminase	HA51_RS18470
hutG'	N-formylglutamate amidohydrolase	HA51_RS18480
Alternative steps:
aapJ	L-histidine ABC transporter, substrate-binding component AapJ	HA51_RS24860
aapM	L-histidine ABC transporter, permease component 2 (AapM)	HA51_RS24870	HA51_RS15495
aapP	L-histidine ABC transporter, ATPase component AapP	HA51_RS24875	HA51_RS14850
aapQ	L-histidine ABC transporter, permease component 1 (AapQ)	HA51_RS24865	HA51_RS07990
Ac3H11_2554	ABC transporter for L-Histidine, permease component 2	HA51_RS25150	HA51_RS15495
Ac3H11_2555	L-histidine ABC transporter, substrate-binding component 2	HA51_RS02660	HA51_RS25145
Ac3H11_2560	L-histidine ABC transporter, ATPase component	HA51_RS17920	HA51_RS00820
Ac3H11_2561	L-histidine ABC transporter, permease component 1	HA51_RS17925
Ac3H11_2562	L-histidine ABC transporter, substrate-binding component 1
bgtA	L-histidine ABC transporter, ATPase component BgtA	HA51_RS11195	HA51_RS09880
bgtB	L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
braC	ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC	HA51_RS15845	HA51_RS18910
braD	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD)	HA51_RS15840	HA51_RS18905
braE	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE)	HA51_RS18900	HA51_RS15835
braF	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF)	HA51_RS18895	HA51_RS15830
braG	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG)	HA51_RS18890	HA51_RS15825
Ga0059261_1577	L-histidine transporter
hisJ	L-histidine ABC transporter, substrate-binding component HisJ	HA51_RS11180	HA51_RS13765
hisM	L-histidine ABC transporter, permease component 1 (HisM)	HA51_RS11190	HA51_RS07730
hisP	L-histidine ABC transporter, ATPase component HisP	HA51_RS11195	HA51_RS25180
hisQ	L-histidine ABC transporter, permease component 2 (HisQ)	HA51_RS11185	HA51_RS12100
hutG	N-formiminoglutamate formiminohydrolase
hutV	L-histidine ABC transporter, ATPase component HutV	HA51_RS20990	HA51_RS18350
hutW	L-histidine ABC transporter, permease component HutW	HA51_RS20995	HA51_RS09235
hutX	L-histidine ABC transporter, substrate-binding component HutX
LAT2	L-histidine transporter
LHT	L-histidine transporter
natA	L-histidine ABC transporter, ATPase component 1 (NatA)	HA51_RS18895	HA51_RS09630
natB	L-histidine ABC transporter, substrate-binding component NatB
natC	L-histidine ABC transporter, permease component 1 (NatC)
natD	L-histidine ABC transporter, permease component 2 (NatD)	HA51_RS15840	HA51_RS07075
natE	L-histidine ABC transporter, ATPase component 2 (NatE)	HA51_RS18890	HA51_RS15825
PA5503	L-histidine ABC transporter, ATPase component	HA51_RS07930	HA51_RS23085
PA5504	L-histidine ABC transporter, permease component	HA51_RS07935	HA51_RS14015
PA5505	L-histidine ABC transporter, substrate-binding component	HA51_RS06735	HA51_RS23095
permease	L-histidine permease	HA51_RS17310	HA51_RS22655
PTR2	L-histidine:H+ symporter
S15A3	L-histidine transporter
SLC38A3	L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.