GapMind for catabolism of small carbon sources

 

2-deoxy-D-ribose catabolism in Rhodococcus qingshengii djl-6-2

Best path

deoP, deoK, deoC, adh, ackA, pta

Rules

Overview: Deoxyribose utilization in GapMind is based on MetaCyc pathways 2-deoxy-D-ribose degradation I via deoxyribose 5-phosphate aldolase (link) and pathway II via oxidation to 2-deoxy-3-dehydro-D-ribonate (link).

19 steps (15 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
deoP deoxyribose transporter
deoK deoxyribokinase C1M55_RS18645 C1M55_RS26600
deoC deoxyribose-5-phosphate aldolase C1M55_RS08075
adh acetaldehyde dehydrogenase (not acylating) C1M55_RS09155 C1M55_RS09205
ackA acetate kinase C1M55_RS07470 C1M55_RS07295
pta phosphate acetyltransferase C1M55_RS07465
Alternative steps:
aacS acetoacetyl-CoA synthetase C1M55_RS09195 C1M55_RS04410
acs acetyl-CoA synthetase, AMP-forming C1M55_RS02775 C1M55_RS25270
ald-dh-CoA acetaldehyde dehydrogenase, acylating C1M55_RS29420 C1M55_RS04080
atoA acetoacetyl-CoA transferase, A subunit C1M55_RS09285 C1M55_RS26645
atoB acetyl-CoA C-acetyltransferase C1M55_RS28700 C1M55_RS07210
atoD acetoacetyl-CoA transferase, B subunit C1M55_RS26640 C1M55_RS09290
deoxyribonate-dehyd 2-deoxy-D-ribonate 3-dehydrogenase C1M55_RS03670 C1M55_RS10010
deoxyribonate-transport 2-deoxy-D-ribonate transporter C1M55_RS00110
drdehyd-alpha 2-deoxy-D-ribose dehydrogenase, alpha subunit C1M55_RS30100 C1M55_RS16880
drdehyd-beta 2-deoxy-D-ribose dehydrogenase, beta subunit
drdehyd-cytc 2-deoxyribose-D dehydrogenase, cytochrome c component
garK glycerate 2-kinase C1M55_RS17755
ketodeoxyribonate-cleavage 2-deoxy-3-keto-D-ribonate cleavage enzyme

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory