GapMind for catabolism of small carbon sources

 

D-galactose catabolism in Rhodobacter viridis JA737

Best path

galP, galK, galT, galE, pgmA

Rules

Overview: Galactose utilization in GapMind is based on MetaCyc pathways lactose and galactose degradation I via tagatose 6-phosphate (link), the Leloir pathway via UDP-galactose (link), and the oxidative pathway via D-galactonate (link). Pathway IV via galactitol (link) is not reported in prokaryotes and is not included. (There is no pathway III.)

48 steps (22 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
galP galactose:H+ symporter GalP
galK galactokinase (-1-phosphate forming)
galT UDP-glucose:alpha-D-galactose-1-phosphate uridylyltransferase
galE UDP-glucose 4-epimerase C8J30_RS04025 C8J30_RS03845
pgmA alpha-phosphoglucomutase C8J30_RS02140 C8J30_RS11560
Alternative steps:
BPHYT_RS16925 galactose ABC transporter, permease component C8J30_RS10880 C8J30_RS14100
BPHYT_RS16930 galactose ABC transporter, ATPase component C8J30_RS14105 C8J30_RS10885
BPHYT_RS16935 galactose ABC transporter, substrate-binding component
CeSWEET1 galactose transporter
chvE galactose ABC transporter, substrate-binding component ChvE C8J30_RS13470
dgoA 2-dehydro-3-deoxy-6-phosphogalactonate aldolase C8J30_RS09810
dgoD D-galactonate dehydratase C8J30_RS09805
dgoK 2-dehydro-3-deoxygalactonokinase
gal2 galactose transporter
galactonolactonase galactonolactonase (either 1,4- or 1,5-lactone)
galdh D-galactose 1-dehydrogenase (forming 1,4- or 1,5-lactones) C8J30_RS14495 C8J30_RS03120
gatY D-tagatose-1,6-bisphosphate aldolase, catalytic subunit (GatY/KbaY) C8J30_RS02175
gatZ D-tagatose-1,6-bisphosphate aldolase, chaperone subunit (GatZ/KbaZ)
gguA galactose ABC transporter, ATPase component GguA C8J30_RS14105 C8J30_RS10885
gguB galactose ABC transporter, permease component GguB C8J30_RS04455
glcS galactose ABC transporter, substrate-binding component GlcS
glcT galactose ABC transporter, permease component 1 (GlcT)
glcU galactose ABC transporter, permease component 2 (GlcU)
glcV galactose ABC transporter, ATPase component (GlcV) C8J30_RS08100 C8J30_RS09730
HP1174 Na+-dependent galactose transporter
lacA galactose-6-phosphate isomerase, lacA subunit
lacB galactose-6-phosphate isomerase, lacB subunit
lacC D-tagatose-6-phosphate kinase C8J30_RS11270 C8J30_RS09075
lacD D-tagatose-1,6-bisphosphate aldolase (monomeric)
lacP galactose:H+ symporter
mglA galactose ABC transporter, ATPase component MglA C8J30_RS14105 C8J30_RS10885
mglB galactose ABC transporter, substrate-binding component MglB C8J30_RS14110
mglC galactose ABC transporter, permease component MglC C8J30_RS14100 C8J30_RS10880
MST1 galactose:H+ symporter
PfGW456L13_1894 ABC transporter for D-Galactose and D-Glucose, periplasmic substrate-binding component
PfGW456L13_1895 ABC transporter for D-Galactose and D-Glucose, permease component 1
PfGW456L13_1896 ABC transporter for D-Galactose and D-Glucose, permease component 2 C8J30_RS01980
PfGW456L13_1897 ABC transporter for D-Galactose and D-Glucose, ATPase component C8J30_RS01990 C8J30_RS09730
ptcA galactose PTS system, EIIA component
ptcB galactose PTS system, EIIB component
ptcEIIC galactose PTS system, EIIC component
sglS sodium/galactose cotransporter
SGLT1 sodium/galactose cotransporter
tpi triose-phosphate isomerase C8J30_RS01230 C8J30_RS02960
yjtF galactose ABC transporter, permease component 2 C8J30_RS14100
ytfQ galactose ABC transporter, substrate-binding component
ytfR galactose ABC transporter, ATPase component C8J30_RS14105 C8J30_RS10885
ytfT galactose ABC transporter, permease component 1 C8J30_RS10880 C8J30_RS14100

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory