L-histidine catabolism in Pseudomonas benzenivorans DSM 8628

Best path

hisP, hisM, hisQ, hisJ, hutH, hutU, hutI, hutF, hutG'

Rules

Overview: Histidine utilization in GapMind is based on MetaCyc pathways L-histidine degradation I (link) or II (link). These pathways are very similar. Other pathways in MetaCyc (III-VI) are not complete or are not reported in prokaryotes, so they are not included.

• all:
  • histidine-transport, hutH, hutU, hutI and hutG
  • or histidine-transport, hutH, hutU, hutI, hutH, hutF and hutG'
  • Comment: In pathway I, hutH deaminates histidine to urocanate, hutU hydrates it to 4-imidazolone-5-propanoate, hutI hydrolyzes it to N-formiminoglutamate, and hutG hydrolyzes it to formamide and glutamate. Formamide can be cleaved to formate and ammonia, but it could also be secreted, so this is not described. Similarly, formate may be oxidized to CO2 or secreted. Pathway II also involves conversion to N-formiminoglutamate, but then it is deaminated to N-formylglutamate by hutF, and hydrolyzed to formate and glutamate by hutG'.
• histidine-transport:
  • hisP, hisM, hisQ and hisJ
  • or PA5503, PA5504 and PA5505
  • or Ac3H11_2562, Ac3H11_2561, Ac3H11_2560, Ac3H11_2555 and Ac3H11_2554
  • or natA, natB, natC, natD and natE
  • or aapJ, aapQ, aapM and aapP
  • or braC, braD, braE, braF and braG
  • or hutV, hutW and hutX
  • or bgtA and bgtB
  • or BPHYT_RS24000, BPHYT_RS24005, BPHYT_RS24010 and BPHYT_RS24015
  • or permease
  • or Ga0059261_1577
  • or S15A3
  • or LAT2
  • or LHT
  • or SLC38A3
  • or PTR2
  • Comment: Transporters were identified using query: transporter:histidine:L-histidine:his

48 steps (35 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
hisP	L-histidine ABC transporter, ATPase component HisP	BLS63_RS24435	BLS63_RS01135
hisM	L-histidine ABC transporter, permease component 1 (HisM)	BLS63_RS24450	BLS63_RS01120
hisQ	L-histidine ABC transporter, permease component 2 (HisQ)	BLS63_RS24445	BLS63_RS01125
hisJ	L-histidine ABC transporter, substrate-binding component HisJ	BLS63_RS24440	BLS63_RS01130
hutH	histidine ammonia-lyase	BLS63_RS05540	BLS63_RS05225
hutU	urocanase	BLS63_RS05545
hutI	imidazole-5-propionate hydrolase	BLS63_RS05530
hutF	N-formiminoglutamate deiminase	BLS63_RS05565
hutG'	N-formylglutamate amidohydrolase	BLS63_RS05525
Alternative steps:
aapJ	L-histidine ABC transporter, substrate-binding component AapJ	BLS63_RS08600
aapM	L-histidine ABC transporter, permease component 2 (AapM)	BLS63_RS08610	BLS63_RS18545
aapP	L-histidine ABC transporter, ATPase component AapP	BLS63_RS08615	BLS63_RS23600
aapQ	L-histidine ABC transporter, permease component 1 (AapQ)	BLS63_RS08605	BLS63_RS11280
Ac3H11_2554	ABC transporter for L-Histidine, permease component 2	BLS63_RS01120	BLS63_RS24450
Ac3H11_2555	L-histidine ABC transporter, substrate-binding component 2	BLS63_RS01130	BLS63_RS08500
Ac3H11_2560	L-histidine ABC transporter, ATPase component	BLS63_RS00800	BLS63_RS07200
Ac3H11_2561	L-histidine ABC transporter, permease component 1	BLS63_RS09905	BLS63_RS00805
Ac3H11_2562	L-histidine ABC transporter, substrate-binding component 1
bgtA	L-histidine ABC transporter, ATPase component BgtA	BLS63_RS24435	BLS63_RS01135
bgtB	L-histidine ABC transporter, fused substrate-binding and permease components (BgtB/BgtAB)
BPHYT_RS24000	L-histidine ABC transporter, substrate-binding component	BLS63_RS26095	BLS63_RS01130
BPHYT_RS24005	L-histidine ABC transporter, permease component 1	BLS63_RS01125	BLS63_RS24445
BPHYT_RS24010	L-histidine ABC transporter, permease component 2	BLS63_RS01120	BLS63_RS26105
BPHYT_RS24015	L-histidine ABC transporter, ATPase component	BLS63_RS24435	BLS63_RS01135
braC	ABC transporter for glutamate, histidine, arginine, and other amino acids, substrate-binding component BraC	BLS63_RS23775	BLS63_RS09655
braD	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 1 (BraD)	BLS63_RS23780	BLS63_RS09650
braE	ABC transporter for glutamate, histidine, arginine, and other amino acids, permease component 2 (BraE)	BLS63_RS09645	BLS63_RS23785
braF	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 1 (BraF)	BLS63_RS09640	BLS63_RS23790
braG	ABC transporter for glutamate, histidine, arginine, and other amino acids, ATPase component 2 (BraG)	BLS63_RS23795	BLS63_RS09635
Ga0059261_1577	L-histidine transporter
hutG	N-formiminoglutamate formiminohydrolase
hutV	L-histidine ABC transporter, ATPase component HutV	BLS63_RS14530	BLS63_RS04335
hutW	L-histidine ABC transporter, permease component HutW	BLS63_RS14535	BLS63_RS04330
hutX	L-histidine ABC transporter, substrate-binding component HutX
LAT2	L-histidine transporter
LHT	L-histidine transporter
natA	L-histidine ABC transporter, ATPase component 1 (NatA)	BLS63_RS09640	BLS63_RS04560
natB	L-histidine ABC transporter, substrate-binding component NatB
natC	L-histidine ABC transporter, permease component 1 (NatC)
natD	L-histidine ABC transporter, permease component 2 (NatD)	BLS63_RS09650	BLS63_RS23780
natE	L-histidine ABC transporter, ATPase component 2 (NatE)	BLS63_RS09635	BLS63_RS23795
PA5503	L-histidine ABC transporter, ATPase component	BLS63_RS14875	BLS63_RS23600
PA5504	L-histidine ABC transporter, permease component	BLS63_RS14880
PA5505	L-histidine ABC transporter, substrate-binding component	BLS63_RS14885
permease	L-histidine permease
PTR2	L-histidine:H+ symporter
S15A3	L-histidine transporter
SLC38A3	L-histidine:Na+ symporter

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.