L-valine catabolism in Amycolatopsis xylanica CPCC 202699

GapMind for catabolism of small carbon sources

L-valine catabolism in Amycolatopsis xylanica CPCC 202699

Best path

livF, livG, livJ, livH, livM, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, pccA, pccB, epi, mcm-large, mcm-small

Rules

Overview: Valine degradation in GapMind is based on MetaCyc pathway L-valine degradation I (link). The other pathways do not produce any fixed carbon and are not included.

all: valine-transport, BKD, acdH, ech, bch, mmsB, mmsA and propionyl-CoA-degradation

Comment: An aminotransferase (not represented) forms 3-methyl-2-oxobutanoate, the decarboxylating alpha-ketoacid dehydrogenase (BKD) forms isobutanoyl-CoA, dehydrogenase acdH forms methylacrylyl-CoA (2-methylprop-2-enoyl-CoA), the hydratase ech forms (S)-3-hydroxy-isobutaonoyl-CoA, a hydrolase forms (S)-3-hydroxy-isobutanoate, a dehydrogenase forms (S)-methylmalonate semialdehyde (2-methyl-3-oxopropanoate), and a decarboxylating dehydrogenase forms propionyl-CoA.

BKD:

bkdA, bkdB, bkdC and lpd
or vorA, vorB and vorC
or ofoA and ofoB
or ofo
Comment: These decarboxylating dehydrogenases act on 4-methyl-2-oxopentanoate, 3-methyl-2-oxobutanoate (2-oxoisovalerate) and (S)-3-methyl-2-oxopentanoate and are known as the branched-chain alpha-ketoacid dehydrogenases. They can pass electrons to NAD (EC 1.2.1.25) or to ferredoxin (EC 1.2.7.7). The NAD-dependent enzyme is the sum of three activities: EC 1.2.4.4 (the 4-methyl-2-oxopentanoate dehydrogenase, with transfer to the lipopoyllysine residue of 2.3.1.168) which is itself heteromeric, with alpha and beta subunits; EC 2.3.1.168 (dihydrolipoyllysine-residue (3-methylbutanoyl)transferase); and EC 1.8.1.4 (dihydrolipoyl dehydrogenase, transferring electrons to NAD). The well-characterized ferredoxin-dependent enzymes have 3 subunits (vorABC) or 2 subunits (ofoAB). Genetic data identified a fused ferredoxin-dependent enzyme with just 1 subunit (ofo).

propionyl-CoA-degradation:

prpC, prpD, acn and prpB
or prpC, acnD, prpF, acn and prpB
or propionyl-CoA-carboxylase, epi and methylmalonyl-CoA-mutase
or pco, hpcD, dddA and iolA
Comment: In 2-methylcitrate cycle I, propionyl-CoA is combined with oxalacetate (by prpC) to give methylcitrate, dehydrated to cis-2-methylaconitate by prpD, hydrated to (2R,3S)-2-methylisocitrate, and a lyase produces pyruvate and succinate. (We consider succinate as a central intermediate, as most organisms can activate it to succinyl-CoA or can oxidize it to fumarate and convert that to oxaloacetate.) In 2-methylcitrate cycle II, a different dehydratase (acnD) and an isomerase (prpF) replace the dehydratase prpD; acnD dehydrates (2S,3S)-2-methylcitrate to 2-methyl-trans-aconitate, and prpF isomerizes it to cis-2-methylaconitate. In propanoyl CoA degradation I, propionyl-CoA carboxylase forms (S)-methylmalonyl-CoA, methylmalonyl-CoA epimerase forms (R)-methylmalonyl-CoA, and methylmalonyl-CoA mutase forms succinyl-CoA, which is a central metabolite. (Note that methylmalonyl-CoA mutase requires adenosylcobamide, a form of vitamin B12, for activity.) In propanoyl-CoA degradation II: propionyl-CoA is oxidized to acrylyl-CoA by pco, hydrated to 3-hydroxypropionyl-CoA, hydrolzed to 3-hydroxypropionate, oxidized to 3-oxopropionate (malonate semialdehyde), and oxidized to acetyl-CoA and CO2.

methylmalonyl-CoA-mutase:

mcmA
or mcm-large and mcm-small
Comment: methylmalonyl-CoA mutase has a catalytic domain and a B12-binding domain. These are usually found in the same protein, which we call mcmA. In Metallosphaera and Pyrococcus, the B12-binding domain is a separate subunit. In Propionibacterium and Methylorubrum, there is an additional subunit with a catalytic domain only; this may have a protective role (PMID:14734568) and is not described here. There's also a mcm-interacting GTPase (known as MeaB or YgfD) that loads B12 onto mcm and protects it from inactivation (see PMC4631608); this is not described here. Some fused mcm proteins include a MeaB domain as well (i.e., Q8F222, Q8Y2U5).

propionyl-CoA-carboxylase:

pccA and pccB
or pccA1, pccA2 and pccB
Comment: propionyl-CoA carboxylase is a heteromer, usually with alpha and beta subunits pccAB. Haloferax mediterranei has a third subunit as well (pccX), which is not described here. Acidianus brierleyi has a diverged pccA split into two pieces.

valine-transport:

livF, livG, livJ, livH and livM
or natA, natB, natC, natD and natE
or Bap2
or brnQ
or phtJ
or bcaP
Comment: Transporters were identified using query: transporter:valine:L-valine:val

47 steps (37 with candidates)

Or see definitions of steps

Step	Description	Best candidate	2nd candidate
livF	L-valine ABC transporter, ATPase component 1 (LivF/BraG)	BLV57_RS38600	BLV57_RS30160
livG	L-valine ABC transporter, ATPase component 2 (LivG/BraF)	BLV57_RS38605	BLV57_RS30165
livJ	L-valine ABC transporter, substrate-binding component (LivJ/LivK/BraC/BraC3)	BLV57_RS36495
livH	L-valine ABC transporter, permease component 1 (LivH/BraD)	BLV57_RS38615	BLV57_RS30170
livM	L-valine ABC transporter, permease component 2 (LivM/BraE)	BLV57_RS38610	BLV57_RS05265
bkdA	branched-chain alpha-ketoacid dehydrogenase, E1 component alpha subunit	BLV57_RS12020	BLV57_RS41245
bkdB	branched-chain alpha-ketoacid dehydrogenase, E1 component beta subunit	BLV57_RS41240	BLV57_RS12025
bkdC	branched-chain alpha-ketoacid dehydrogenase, E2 component	BLV57_RS41235	BLV57_RS12030
lpd	branched-chain alpha-ketoacid dehydrogenase, E3 component	BLV57_RS29630	BLV57_RS41645
acdH	isobutyryl-CoA dehydrogenase	BLV57_RS16765	BLV57_RS07910
ech	(S)-3-hydroxybutanoyl-CoA hydro-lyase	BLV57_RS35310	BLV57_RS23875
bch	3-hydroxyisobutyryl-CoA hydrolase	BLV57_RS29490	BLV57_RS29875
mmsB	3-hydroxyisobutyrate dehydrogenase	BLV57_RS04465	BLV57_RS02390
mmsA	methylmalonate-semialdehyde dehydrogenase	BLV57_RS35960	BLV57_RS21830
pccA	propionyl-CoA carboxylase, alpha subunit	BLV57_RS28975	BLV57_RS16770
pccB	propionyl-CoA carboxylase, beta subunit	BLV57_RS29030	BLV57_RS36630
epi	methylmalonyl-CoA epimerase	BLV57_RS14775
mcm-large	methylmalonyl-CoA mutase, large (catalytic) subunit	BLV57_RS28865	BLV57_RS15930
mcm-small	methylmalonyl-CoA mutase, small (adenosylcobamide-binding) subunit	BLV57_RS28865	BLV57_RS15930
Alternative steps:
acn	(2R,3S)-2-methylcitrate dehydratase	BLV57_RS03260
acnD	2-methylcitrate dehydratase (2-methyl-trans-aconitate forming)	BLV57_RS03260
Bap2	L-valine permease Bap2	BLV57_RS20100	BLV57_RS35065
bcaP	L-valine uptake transporter BcaP/CitA	BLV57_RS17200	BLV57_RS29690
brnQ	L-valine:cation symporter BrnQ/BraZ/BraB
dddA	3-hydroxypropionate dehydrogenase	BLV57_RS10945	BLV57_RS16295
hpcD	3-hydroxypropionyl-CoA dehydratase	BLV57_RS35310	BLV57_RS20495
iolA	malonate semialdehyde dehydrogenase (CoA-acylating)	BLV57_RS35960	BLV57_RS21830
mcmA	methylmalonyl-CoA mutase, fused catalytic and adenosylcobamide-binding components	BLV57_RS28865	BLV57_RS23065
natA	L-valine ABC transporter, ATPase component 1 (NatA)	BLV57_RS38605	BLV57_RS30165
natB	L-valine ABC transporter, substrate-binding component NatB
natC	L-valine ABC transporter, permease component 1 (NatC)
natD	L-valine ABC transporter, permease component 2 (NatD)	BLV57_RS38615
natE	L-valine ABC transporter, ATPase component 2 (NatE)	BLV57_RS38600	BLV57_RS30160
ofo	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, fused	BLV57_RS37315
ofoA	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit OfoA
ofoB	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit OfoB	BLV57_RS27100
pccA1	propionyl-CoA carboxylase, biotin carboxyl carrier subunit	BLV57_RS28975	BLV57_RS13960
pccA2	propionyl-CoA carboxylase, biotin carboxylase subunit
pco	propanyl-CoA oxidase	BLV57_RS17700	BLV57_RS20510
phtJ	L-valine uptake permease PhtJ
prpB	2-methylisocitrate lyase	BLV57_RS35445	BLV57_RS03460
prpC	2-methylcitrate synthase	BLV57_RS07895	BLV57_RS08055
prpD	2-methylcitrate dehydratase
prpF	methylaconitate isomerase
vorA	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, alpha subunit VorA
vorB	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, beta subunit VorB	BLV57_RS27105
vorC	branched-chain alpha-ketoacid:ferredoxin oxidoreductase, gamma subunit VorC

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources