catabolism of small carbon sources in Brevibacterium jeotgali SJ5-8

GapMind for catabolism of small carbon sources

catabolism of small carbon sources in Brevibacterium jeotgali SJ5-8

Pathways are sorted by completeness. Sort by name instead.

Pathway	Steps
propionate	lctP, prpE, prpC, prpD, acn, prpB
fumarate	SLC26dg
glutamate	gltL, gluB, gluC, gluD, gdhA
L-lactate	lctP, lutA, lutB, lutC
ethanol	etoh-dh-nad, adh, acs
asparagine	ans, dauA
alanine	alsT
aspartate	dauA
2-oxoglutarate	kgtP
succinate	dauA
isoleucine	Bap2, bkdA, bkdB, bkdC, lpd, acdH, ech, ivdG, fadA, prpC, prpD, acn, prpB
valine	Bap2, bkdA, bkdB, bkdC, lpd, acdH, ech, bch, mmsB, mmsA, prpC, prpD, acn, prpB
leucine	leuT, ilvE, bkdA, bkdB, bkdC, lpd, liuA, liuB, liuD, liuC, liuE, atoA, atoD, atoB
threonine	tdcC, ltaE, adh, acs, gcvP, gcvT, gcvH, lpd
arginine	bgtB, artP, aruH, aruI, kauB, gbuA, gabT, gabD
lysine	lysP, cadA, patA, patD, davT, davD, gcdG, gcdH, ech, fadB, atoB
histidine	permease, hutH, hutU, hutI, hutG
putrescine	puuP, patA, patD, gabT, gabD
citrate	SLC13A5, acn, icd
glycerol	glpF, glpK, glpD, tpi
deoxyinosine	nupA, nupB, nupC', bmpA, deoD, deoB, deoC, adh, acs
acetate	actP, acs
glucosamine	gamP, nagB
D-lactate	lctP, D-LDH
mannose	manP, manA
D-serine	cycA, dsdA
serine	serP, sdaB
proline	proY, prdF, prdA, prdB, prdC, davT, davD, gcdG, gcdH, ech, fadB, atoB
thymidine	nupG, deoA, deoB, deoC, adh, acs
deoxyribonate	deoxyribonate-transport, deoxyribonate-dehyd, ketodeoxyribonate-cleavage, garK, atoA, atoD, atoB
fructose	fruII-ABC, 1pfk, fba, tpi
glucose	ptsG-crr
glucose-6-P	uhpT
L-malate	sdlC
pyruvate	SLC5A8
deoxyribose	deoP, deoK, deoC, adh, acs
tryptophan	aroP, tnaA
NAG	nagEcba, nagA, nagB
cellobiose	cdt, cbp, pgmA, glk
mannitol	PLT5, mt1d, mak, manA
trehalose	TRET1, PsTP, pgmA, glk
galactose	galP, galK, galT, galE, pgmA
sucrose	sut, SUS, scrK, galU, pgmA
D-alanine	cycA, dadA
maltose	susB, ptsG-crr
ribose	rbsU, rbsK
sorbitol	mtlA, srlD
xylitol	fruI, x5p-reductase
tyrosine	aroP, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
gluconate	gntT, gntK, gnd
xylose	xylT, xylA, xylB
lactose	lacP, lacZ, galK, galT, galE, pgmA, glk
4-hydroxybenzoate	pcaK, pobA, praA, xylF, mhpD, mhpE, adh, acs
arabinose	araE, araA, araB, araD
citrulline	AO353_03055, AO353_03050, AO353_03045, AO353_03040, arcB, arcC, odc, patA, patD, gabT, gabD
glucuronate	exuT, udh, gci, kdgD, dopDH
rhamnose	rhaT, LRA1, LRA2, LRA3, LRA4, aldA
fucose	fucP, fucU, fucI, fucK, fucA, tpi, aldA
phenylalanine	aroP, PAH, PCBD, QDPR, HPD, hmgA, maiA, fahA, atoA, atoD, atoB
galacturonate	exuT, udh, gli, gci, kdgD, dopDH
myoinositol	iolT, iolG, iolE, iolD, iolB, iolC, iolJ, mmsA, tpi
phenylacetate	paaT, paaK, paaA, paaB, paaC, paaE, paaG, paaZ1, paaZ2, paaJ1, paaF, paaH, paaJ2

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources