Align methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate WP_057507595.1 ABB28_RS05085 methylcrotonoyl-CoA carboxylase
Query= BRENDA::Q9I297 (535 letters) >NCBI__GCF_001431535.1:WP_057507595.1 Length = 536 Score = 774 bits (1999), Expect = 0.0 Identities = 385/536 (71%), Positives = 442/536 (82%), Gaps = 1/536 (0%) Query: 1 MAILHTQINPRSAEFAANAATMLEQVNALRTLLGRIHEGGGSAAQARHSARGKLLVRERI 60 M+ L TQ++ S F +N + M V+ L L R GG AA+++H+ARGKLLVRERI Sbjct: 1 MSTLSTQLHAGSEPFESNRSAMQAVVDDLHATLARTALGGSEAARSKHTARGKLLVRERI 60 Query: 61 NRLLDPGSPFLELSALAAHEVYGEEVAAAGIVAGIGRVEGVECMIVGNDATVKGGTYYPL 120 + LLD GS FLE++ LAAH +Y + V AG+VAGIGRV GVEC+IV NDATVKGGTYYP+ Sbjct: 61 DALLDAGSAFLEIAPLAAHGMYEDAVPCAGVVAGIGRVSGVECVIVANDATVKGGTYYPM 120 Query: 121 TVKKHLRAQAIALENRLPCIYLVDSGGANLPRQDEVFPDREHFGRIFFNQANMSARGIPQ 180 TVKKHLRAQ IA +NRLPCIYLVDSGGA LP QDEVFPDR+HFGRIF+NQAN+SA+GIPQ Sbjct: 121 TVKKHLRAQEIAEQNRLPCIYLVDSGGAFLPLQDEVFPDRDHFGRIFYNQANLSAQGIPQ 180 Query: 181 IAVVMGSCTAGGAYVPAMSDETVMVREQATIFLAGPPLVKAATGEVVSAEELGGADVHCK 240 IA VMGSCTAGGAYVPAMSDETV+VREQ TIFL GPPLVKAATGEVVSAE+LGGADVH + Sbjct: 181 IACVMGSCTAGGAYVPAMSDETVIVREQGTIFLGGPPLVKAATGEVVSAEDLGGADVHTR 240 Query: 241 VSGVADHYAEDDDHALAIARRCVANLNWRK-QGQLQCRAPRAPLYPAEELYGVIPADSKQ 299 +SGVADH A++D ALA R +A LNW K + + + P+ PL+P ++LYGVIPAD+++ Sbjct: 241 LSGVADHMADNDLQALARVRAIIAQLNWCKPEPAMALQPPQEPLFPPQDLYGVIPADTRK 300 Query: 300 PYDVREVIARLVDGSEFDEFKALFGTTLVCGFAHLHGYPIAILANNGILFAEAAQKGAHF 359 P+DVREVIARLVDGS FDEFK +G+TLV GFAHLHGYP+ I+ANNGILF+E+A KGAHF Sbjct: 301 PFDVREVIARLVDGSRFDEFKPRYGSTLVTGFAHLHGYPVGIIANNGILFSESALKGAHF 360 Query: 360 IELACQRGIPLLFLQNITGFMVGQKYEAGGIAKHGAKLVTAVACARVPKFTVLIGGSFGA 419 IEL QR IPL+FLQNITGFMVG+KYE GGIAK GAKLV AVACA+VPKFTV+IGGSFGA Sbjct: 361 IELCTQRNIPLVFLQNITGFMVGRKYEQGGIAKDGAKLVMAVACAKVPKFTVVIGGSFGA 420 Query: 420 GNYGMCGRAYDPRFLWMWPNARIGVMGGEQAAGVLAQVKREQAERAGQQLGVEEEAKIKA 479 GNYGMCGRAY P FLWMWPNARIGVMGGEQAA VLA VKR+ E G EE KA Sbjct: 421 GNYGMCGRAYSPNFLWMWPNARIGVMGGEQAASVLATVKRDGIEAKGGAWAAAEEEAFKA 480 Query: 480 PILEQYEHQGHPYYSSARLWDDGVIDPAQTREVLALALSAALNAPIEPTAFGVFRM 535 PI +Q+EHQGHPYY+SARLWDDG+IDPA TR VLALALSA+LNAP T FGVFRM Sbjct: 481 PIRDQFEHQGHPYYASARLWDDGIIDPADTRRVLALALSASLNAPARETRFGVFRM 536 Lambda K H 0.321 0.137 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 976 Number of extensions: 36 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 535 Length of database: 536 Length adjustment: 35 Effective length of query: 500 Effective length of database: 501 Effective search space: 250500 Effective search space used: 250500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory