Align α-glucosidase (SusB;Aglu;BFO_2272) (EC 3.2.1.20) (characterized)
to candidate WP_057507039.1 ABB28_RS02145 alpha-glucosidase
Query= CAZy::AEW20125.1 (708 letters) >NCBI__GCF_001431535.1:WP_057507039.1 Length = 684 Score = 517 bits (1331), Expect = e-151 Identities = 301/710 (42%), Positives = 407/710 (57%), Gaps = 65/710 (9%) Query: 10 LFFLFSFLVSAPFTMRAEELKSPDGNLLMRFEVNGEGTPVYELTYKRKPVIKPSRLGLEI 69 L L + V T+ + ++SP L + +V+G GT Y++ V+ S+LG E+ Sbjct: 18 LLLLLASPVVLAATVPSASVESPGKILKVTLQVDG-GTARYQVQRLGDTVVDASKLGFEL 76 Query: 70 EGFSLRKAFTLKGVETVTFDETWTPVWGEVKAIRNHYNEMTATLHQADGDRTMV-IRFRL 128 L + F + G T FDETW WGE + RNHYNE+T +L Q GDR + + FR+ Sbjct: 77 RDGRLDRDFEITGSSTRRFDETWEQPWGERRLTRNHYNELTVSLRQTTGDRRRLDVVFRV 136 Query: 129 YDDGLGFRYEFPQQKSLNYFVIKEEYSEFAMAGDHKAFWIP-GDYDTQEYDYTESRLTEI 187 +DDG+GFRY FPQQ L +I EE +EFA+A A+WIP G+ EY Y + L E+ Sbjct: 137 FDDGVGFRYVFPQQPGLAEAIIDEELTEFAIAQHSTAWWIPAGEPIHYEYLYARTPLQEV 196 Query: 188 RGLMQTAITPNTSQTPFSPTGVQTALMMKSDDGLYINLHEAALVDYPCMSLN-LDDQTLT 246 L T IT ++SD GL++ +HEAALVDY M L D Q L Sbjct: 197 P-LAHTPIT------------------LRSDSGLHVAIHEAALVDYAGMWLRRTDGQRLR 237 Query: 247 FQSWLTPDAQGKKGYMQTPCHTPWRTVIVSDDARRILASHLTLNLNEPCKLDDVSWIRPV 306 Q L+P A+G K P TPWRT+ ++D A ++ S L LNLNEP L DVSW++P Sbjct: 238 AQ--LSPSAEGWKVRRALPFATPWRTLQIADRAGGLVESDLILNLNEPNALGDVSWVKPS 295 Query: 307 KYIGVWWEMISGKSTWAYTNDLPSVHLDVTDYTKMKPNGTHAANNEKVRRYIDFAAKHGF 366 KY+GVWW M + +WA HAA K R+ IDFAA +GF Sbjct: 296 KYLGVWWSMHLDEESWA-------------------TGPRHAATTAKTRKVIDFAAANGF 336 Query: 367 DQVLVEGWNIGWE-DWFGNSKDYVFDFVTPYPDFDLKALNEYAHSKGVKLMMHHETSSSV 425 VLVEGWN GW+ +W GN D FDF PDFD++AL+ Y KGV L+ HHET ++ Sbjct: 337 RGVLVEGWNPGWDGNWVGNGYD--FDFTRATPDFDIEALSAYGAKKGVHLVGHHETGCAI 394 Query: 426 RNYERQMEKAYKLMNDYGYNAVKSGYV---GNIIPRG--------EYHYGQWMVNHYLYA 474 +YE Q+ A L G ++ K+GYV G + R E+H GQ+M H+L Sbjct: 395 DHYEDQLGAALDLYARLGVDSFKTGYVCDDGQVDRRNPAGGPLLREWHDGQFMARHHLKV 454 Query: 475 VKKAAEHRIMVNAHEAVRPTGLCRTYPNLIGNEAARGGEYESFGG--NKVFHTTILPFTR 532 V++AA R+ VN HE ++ TGL RTYPN + E ARG EY ++G N H L FTR Sbjct: 455 VQEAAARRLAVNPHEPIKDTGLRRTYPNWVSREGARGMEYNAWGQPPNPPEHEVNLVFTR 514 Query: 533 LMGGPMDYTPGIFETRLEKVNPGNNSYVHSTLARQLALYVTMYSPLQMAADLPENYERFM 592 ++ GPMDYTPG+ + + + STLARQLALYV +YSP+QMAADLPE+Y + + Sbjct: 515 MLSGPMDYTPGVLSLKGR-----HGQAIPSTLARQLALYVVLYSPIQMAADLPEHYLQHL 569 Query: 593 DAFQFIKDVAIDWDDSKYLEAEPGRYITVARKAKGTNNWFVGCTANEDGHLSNVSLDFLD 652 DAFQFIKDVA+DW+ S+ L+ E G ++T+ R+ + + +WF+G +E G + VSL FL+ Sbjct: 570 DAFQFIKDVAVDWEQSRVLDGEVGDFVTIVRRDRNSRDWFLGSITDEHGRVLPVSLGFLE 629 Query: 653 PGRKYTATIYADAPTAHYEKNPQAYTIKQMKVTNKSKLTLRAASGGGYAI 702 PG +Y A IY D A + NP A+ + +VT+ LTL GGG AI Sbjct: 630 PGVRYRAEIYRDGDGADFRSNPFAFARETREVTSADALTLVLGPGGGQAI 679 Lambda K H 0.319 0.135 0.417 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1383 Number of extensions: 83 Number of successful extensions: 10 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 708 Length of database: 684 Length adjustment: 39 Effective length of query: 669 Effective length of database: 645 Effective search space: 431505 Effective search space used: 431505 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory