Alignments for a candidate for lctP in Stenotrophomonas chelatiphaga DSM 21508

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Stenotrophomonas chelatiphaga DSM 21508

Align L-lactate permease (characterized, see rationale)
to candidate WP_057509294.1 ABB28_RS14460 L-lactate permease

Query= uniprot:Q8EIL2
         (545 letters)



>NCBI__GCF_001431535.1:WP_057509294.1
          Length = 540

 Score =  419 bits (1078), Expect = e-121
 Identities = 221/534 (41%), Positives = 336/534 (62%), Gaps = 10/534 (1%)

Query: 3   WTQTYTPLGSLWLTAIVALLPIVFFFLALTVLKLKGHIAGALTLLIALAVAIITYKMPVS 62
           W Q Y PLG+  L+A++A LP++ F LALTVL+LKG  A  L +++++ V+ + + MP  
Sbjct: 2   WQQNYDPLGNPNLSALLAALPVLVFLLALTVLRLKGLTAALLAVVVSVLVSALVFGMPAG 61

Query: 63  IALASAIYGFSYGLWPIAWIIITAVFLYKITVKTGQFEIIRSSVISVTEDQRLQMLLVGF 122
               + + G + G++PI++I++ AV+LYK+ +++G+F++IR S+ +++EDQR+Q+LL+ F
Sbjct: 62  TIAGAGLLGIANGVFPISFIVLMAVWLYKLAIRSGKFDVIRGSIATISEDQRIQVLLIAF 121

Query: 123 SFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAMGIPIIVAGQV 182
            FG FLEGAAGFG P+AI AALLV LGF PL AA LCL+AN A  A+GA+GIP++V  Q 
Sbjct: 122 CFGGFLEGAAGFGVPIAICAALLVELGFRPLKAAMLCLLANGAAGAYGAIGIPVVVGAQQ 181

Query: 183 SSLDPFHIGQLAGRQLPILSIIVPFWLIAMMDGIRGIRQTWPATLVAGVSFAVTQFLTSN 242
             +    +  +    + +++++ P  L+ M+DG RG+R+TWP  LV G  F+  Q     
Sbjct: 182 GGVSLEAMSLMLMALVQVIALLSPAILVLMLDGWRGVRETWPVLLVVGGVFSGVQTAVLY 241

Query: 243 FIGPELPDITSALVSLICLTLFLKVWQPKEIFTFSGMKQRAVTPKSTFSNGQIFKAWSPF 302
            +GPEL DI   L ++  L  F++VW+P+ I+  +        P   ++  Q+  AWSPF
Sbjct: 242 LLGPELVDIIGPLAAMAALAGFMQVWRPRRIYREA---DTPPGPAQRYTLKQVLLAWSPF 298

Query: 303 IILTAIVTLWSIKDVQL------ALSFATISIEVPYLHNLVIKTAPIVAKETPYAAIYKL 356
            ILT  + LWS+   +       AL+   + + +  L   V +  P+V       A++ +
Sbjct: 299 YILTGAILLWSLPAFKALFAAGGALASTVLHLPIGLLDGRVQELPPLVVNVHTLPAVWNV 358

Query: 357 NLLGAVGTAILIAAMISIVVL-KMSISNALTSFKDTLIELRFPILSIGLVLAFAFVANYS 415
             L A GTAIL+AA++++V+  ++++  +      T  E+  P+ ++ LV+A A++ NYS
Sbjct: 359 GWLNASGTAILVAAVLTVVLSPRLNLRMSGEELAQTAGEMWKPLATVSLVMAVAYITNYS 418

Query: 416 GLSSTLALVLAGTGVAFPFFSPFLGWLGVFLTGSDTSSNALFGALQANTANQIGVTPELL 475
           G SST+ L LA TG  FP  +P +GW+GVF+TGS  +SN LF  LQA TA QIGV   LL
Sbjct: 419 GASSTIGLALAQTGGVFPLLAPVIGWMGVFITGSVVNSNTLFAHLQAVTAAQIGVPEALL 478

Query: 476 VAANTTGGVTGKMISPQSIAVACAATGLAGKESDLFRFTLKHSLFFCTFIGVLT 529
           VAANT GGV  K++SPQSIA+A AA  L G+ES + R TL  SL    ++ + T
Sbjct: 479 VAANTAGGVMAKLVSPQSIAIAAAAVKLVGQESAIMRTTLGISLGLLAYVCLCT 532


Lambda     K      H
   0.327    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 801
Number of extensions: 41
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 545
Length of database: 540
Length adjustment: 35
Effective length of query: 510
Effective length of database: 505
Effective search space:   257550
Effective search space used:   257550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources