Align α,α-trehalase (MSMEG_4535;MSMEG4528) (EC 3.2.1.28) (characterized)
to candidate WP_083491891.1 ABB28_RS03155 glycoside hydrolase family 15 protein
Query= CAZy::ABK72415.1 (668 letters) >NCBI__GCF_001431535.1:WP_083491891.1 Length = 613 Score = 233 bits (594), Expect = 2e-65 Identities = 191/632 (30%), Positives = 287/632 (45%), Gaps = 74/632 (11%) Query: 41 PIADYGFLSDCETTCLISSAGSVEWLCVPRPDSPSVFGAILDR-GAGHFRLGPYGVSVPA 99 PI Y L + + L GS++W C P DSP +F +LD G+F + P Sbjct: 31 PIEQYSALGEGRSVALTGCDGSIDWWCAPNMDSPPLFDRLLDATNGGYFSIAPTEPCT-V 89 Query: 100 ARRYLPGSLILETTWQTHTGWLIVRDALVMG-----PWHDIDTRSRTHRRTPMDWDAEHI 154 RRY S +LET + T +G + ++L G PW + Sbjct: 90 ERRYRAESNVLETVFTTASGRASLTESLNSGNAGRLPWCE-------------------- 129 Query: 155 LLRTVRCVSGTVELVMSCEPAFDYHRVSATWEYSGPAYGEAIARASRNPDSHPTLRLTTN 214 L R + + G V + P S G + R HP L+L Sbjct: 130 LARRIEGLEGEVRFAVRMCPGTRAQSASPYCSSIGEHVVFHVQRLLGVMIHHPALQLQWT 189 Query: 215 LRIGIEGREARARTRLTEGDNVFVALSWSKHP---APQTYE-----EAADKMWKTSEAWR 266 A ++ G V VAL + AP E + D+ WK AW Sbjct: 190 ------DEGASGECTVSAGGRVTVALVVGEDEPLVAPSVEEVDGRIDLTDREWK---AWA 240 Query: 267 QWINVGDFPDHPWRAYLQRSALTLKGLTYSPTGALLAAPTTSLPETPQGERNWDYRYSWI 326 + ++ G + RA RS L LK L YSP+GA+ AA TTSLPE G++N+DYRY+W+ Sbjct: 241 RNVSYGGWD----RATFVRSTLALKLLLYSPSGAIAAAATTSLPERIGGDKNFDYRYAWV 296 Query: 327 RDSTFALWGLYTLGLDREADDFFSFIADVSGANNGERHPLQVMYGVGGERSLVEEELHHL 386 RD+ + + GL+ E+ F+++ RH +V++ + GE+ +EL + Sbjct: 297 RDAGYTIQAFLAAGLEAESKAAFTWL-----LRQLRRHGPKVVFTLDGEKVEPVQEL-PM 350 Query: 387 SGYDNSRPVRIGNGAYNQRQHDIWGTMLDSVYLHAKSREQIPDALWPVLKNQVEEAIKHW 446 GY NS+PV GN A Q QH I+G + ++ + + + A +L + HW Sbjct: 351 HGYRNSQPVVKGNLAGEQHQHGIYGDIFETAFCFVAAGNILDGASAELLSRIADLCADHW 410 Query: 447 KEPDRGIWEVRGEPQHFTSSKIMCWVALDRGSKLAELQGE--KSYAQQWR----AIAEEI 500 + D GIWE+ E +H+T SKI CW AL R +LA+ QG+ + +W+ I+E I Sbjct: 411 RAKDSGIWEL-PELEHYTMSKISCWQALARAVELAD-QGQLPTTCRDRWQRERDRISEWI 468 Query: 501 KADVLARGVDKRGVLTQRYGDDALDASLLLAVLTRFLPADDPRIRATVLAIADELTEDGL 560 + + + D G LDA+L LAV RF D R+ AT+ AI EL + G Sbjct: 469 ETNCWS---DSLQAYEMYPGSGRLDAALALAVRFRFDGRD--RLLATLRAIDRELGQSGF 523 Query: 561 VLRYRVEETDDGLAGEEGTFTICSFWLVSALVEIGEISRAKHLCERLLSFASPLH-LYAE 619 RY G+A EEG F CS+W+ A +G ++ A+ + L + +E Sbjct: 524 HYRY------SGMAEEEGCFIACSYWMAEAYARLGFVADARDRVDALNGALGRCQGVLSE 577 Query: 620 EIEPRTGRHLGNFPQAFTHLALINAVVHVIRA 651 ++P TG +LGN PQ +HLA + A+ + A Sbjct: 578 MVDPATGHYLGNTPQGLSHLAQVMAMATIADA 609 Lambda K H 0.319 0.135 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1107 Number of extensions: 60 Number of successful extensions: 8 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 668 Length of database: 613 Length adjustment: 38 Effective length of query: 630 Effective length of database: 575 Effective search space: 362250 Effective search space used: 362250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory