Align p-hydroxybenzoate hydroxylase; PHBH; 4-hydroxybenzoate 3-monooxygenase; EC 1.14.13.2 (characterized)
to candidate WP_086508579.1 BZY95_RS03375 4-hydroxybenzoate 3-monooxygenase
Query= SwissProt::P20586 (394 letters) >NCBI__GCF_002151265.1:WP_086508579.1 Length = 394 Score = 623 bits (1606), Expect = 0.0 Identities = 303/394 (76%), Positives = 340/394 (86%) Query: 1 MKTQVAIIGAGPSGLLLGQLLHKAGIDNVILERQTPDYVLGRIRAGVLEQGMVDLLREAG 60 MKTQVAIIGAGPSGLLLGQLL + GIDNVILER++ +YVL RIRAGVLEQGMVDLLREAG Sbjct: 1 MKTQVAIIGAGPSGLLLGQLLSRQGIDNVILERKSGEYVLSRIRAGVLEQGMVDLLREAG 60 Query: 61 VDRRMARDGLVHEGVEIAFAGQRRRIDLKRLSGGKTVTVYGQTEVTRDLMEAREACGATT 120 VD RM +GL H+GVE+AF +R RIDL L+GGKTV VYGQTEVTRDLMEAR A G TT Sbjct: 61 VDARMNEEGLPHDGVELAFDNRRVRIDLAGLTGGKTVMVYGQTEVTRDLMEARAAGGGTT 120 Query: 121 VYQAAEVRLHDLQGERPYVTFERDGERLRLDCDYIAGCDGFHGISRQSIPAERLKVFERV 180 +Y+A V+ HDL+ + PYVTF +DGE +RLDCDY+AGCDGFHG+SRQSIP +R+K FE++ Sbjct: 121 IYEADNVQPHDLETDAPYVTFVKDGETVRLDCDYVAGCDGFHGVSRQSIPKDRIKEFEKI 180 Query: 181 YPFGWLGLLADTPPVSHELIYANHPRGFALCSQRSATRSRYYVQVPLSEKVEDWSDERFW 240 YPFGWLGLLADTPPVS ELIYA H RGFALCS RS TRSRYYVQVPL EKVEDWSDERFW Sbjct: 181 YPFGWLGLLADTPPVSDELIYARHERGFALCSMRSPTRSRYYVQVPLDEKVEDWSDERFW 240 Query: 241 TELKARLPSEVAEKLVTGPSLEKSIAPLRSFVVEPMQHGRLFLAGDAAHIVPPTGAKGLN 300 ELK RLP EVA KLVTGP+LEKSIAPLRSFVVEPMQ+GRLFL GDAAHIVPPTGAKGLN Sbjct: 241 AELKRRLPEEVAAKLVTGPALEKSIAPLRSFVVEPMQYGRLFLVGDAAHIVPPTGAKGLN 300 Query: 301 LAASDVSTLYRLLLKAYREGRGELLERYSAICLRRIWKAERFSWWMTSVLHRFPDTDAFS 360 LAASDV+TLYRL++K Y+EGR +L+ YS CL+RIWKAERFSWWMTS+LH+F D + F Sbjct: 301 LAASDVNTLYRLMVKVYQEGRTDLIPNYSQTCLKRIWKAERFSWWMTSLLHKFSDEEGFE 360 Query: 361 QRIQQTELEYYLGSEAGLATIAENYVGLPYEEIE 394 R+QQ EL+Y SEAGL TIAENYVGLPYE +E Sbjct: 361 TRMQQAELDYLTSSEAGLKTIAENYVGLPYESLE 394 Lambda K H 0.321 0.138 0.413 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 585 Number of extensions: 12 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 394 Length of database: 394 Length adjustment: 31 Effective length of query: 363 Effective length of database: 363 Effective search space: 131769 Effective search space used: 131769 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate WP_086508579.1 BZY95_RS03375 (4-hydroxybenzoate 3-monooxygenase)
to HMM TIGR02360 (pobA: 4-hydroxybenzoate 3-monooxygenase (EC 1.14.13.2))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02360.hmm # target sequence database: /tmp/gapView.29540.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02360 [M=390] Accession: TIGR02360 Description: pbenz_hydroxyl: 4-hydroxybenzoate 3-monooxygenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.3e-219 713.3 0.0 3.7e-219 713.2 0.0 1.0 1 lcl|NCBI__GCF_002151265.1:WP_086508579.1 BZY95_RS03375 4-hydroxybenzoate Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_002151265.1:WP_086508579.1 BZY95_RS03375 4-hydroxybenzoate 3-monooxygenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 713.2 0.0 3.7e-219 3.7e-219 1 390 [] 1 390 [. 1 390 [. 1.00 Alignments for each domain: == domain 1 score: 713.2 bits; conditional E-value: 3.7e-219 TIGR02360 1 mktqvaiigaGpsGlllgqllhkaGidavilerksrdyvlgriraGvleqgtvdlleeagvderldreg 69 mktqvaiigaGpsGlllgqll ++Gid+vilerks +yvl+riraGvleqg+vdll+eagvd r+++eg lcl|NCBI__GCF_002151265.1:WP_086508579.1 1 MKTQVAIIGAGPSGLLLGQLLSRQGIDNVILERKSGEYVLSRIRAGVLEQGMVDLLREAGVDARMNEEG 69 9******************************************************************** PP TIGR02360 70 lvheGveiafegekvrvdlkkltggksvlvyGqtevtrdlyeareaaglktvyeadevrlhdlesdrpk 138 l+h+Gve+af++++vr+dl+ ltggk+v+vyGqtevtrdl+ear a g++t+yead+v++hdle+d+p+ lcl|NCBI__GCF_002151265.1:WP_086508579.1 70 LPHDGVELAFDNRRVRIDLAGLTGGKTVMVYGQTEVTRDLMEARAAGGGTTIYEADNVQPHDLETDAPY 138 ********************************************************************* PP TIGR02360 139 vtfekdgeekrldcdfiaGcdGfhGvsrksipaeklkefekvypfGwlGilsetppvsdeliysnserG 207 vtf kdge++rldcd++aGcdGfhGvsr+sip++++kefek+ypfGwlG+l++tppvsdeliy+++erG lcl|NCBI__GCF_002151265.1:WP_086508579.1 139 VTFVKDGETVRLDCDYVAGCDGFHGVSRQSIPKDRIKEFEKIYPFGWLGLLADTPPVSDELIYARHERG 207 ********************************************************************* PP TIGR02360 208 falcslrsetrsryyvqvsltdkvedwsddrfweelkrrldeeaaeklvtgpsieksiaplrsfvaepm 276 falcs+rs trsryyvqv+l++kvedwsd+rfw+elkrrl+ee+a+klvtgp++eksiaplrsfv+epm lcl|NCBI__GCF_002151265.1:WP_086508579.1 208 FALCSMRSPTRSRYYVQVPLDEKVEDWSDERFWAELKRRLPEEVAAKLVTGPALEKSIAPLRSFVVEPM 276 ********************************************************************* PP TIGR02360 277 ryGrlflaGdaahivpptGakGlnlaasdvaylyealleaykekdsaglerysakalarvwkaerfsww 345 +yGrlfl+GdaahivpptGakGlnlaasdv++ly+ +++ y+e++++ + +ys+ +l+r+wkaerfsww lcl|NCBI__GCF_002151265.1:WP_086508579.1 277 QYGRLFLVGDAAHIVPPTGAKGLNLAASDVNTLYRLMVKVYQEGRTDLIPNYSQTCLKRIWKAERFSWW 345 ********************************************************************* PP TIGR02360 346 ltsllhrfpdedefdkkiqqaeleylleseaaqktlaenyvGlpy 390 +tsllh+f de+ f++++qqael+yl++sea++kt+aenyvGlpy lcl|NCBI__GCF_002151265.1:WP_086508579.1 346 MTSLLHKFSDEEGFETRMQQAELDYLTSSEAGLKTIAENYVGLPY 390 ********************************************9 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (390 nodes) Target sequences: 1 (394 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 11.57 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory