Alignments for a candidate for xacD in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for xacD in Halomonas desiderata SP1

Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_086510991.1 BZY95_RS16565 dihydroxy-acid dehydratase

Query= reanno::HerbieS:HSERO_RS05205
         (585 letters)



>NCBI__GCF_002151265.1:WP_086510991.1
          Length = 581

 Score =  981 bits (2536), Expect = 0.0
 Identities = 473/579 (81%), Positives = 529/579 (91%)

Query: 7   DKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRK 66
           D+ R LRSA WFG+ADKNGFMYRSWMKNQGIPDHEF+GKP+IGICNTWSELTPCNAHFRK
Sbjct: 3   DRKRPLRSAQWFGSADKNGFMYRSWMKNQGIPDHEFEGKPIIGICNTWSELTPCNAHFRK 62

Query: 67  IAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLT 126
           +AEHV++G++EAGGFPVEFPVFSNGES LRPTAM TRNLASMDVEESIRGNP+DAVVLL 
Sbjct: 63  LAEHVKKGVLEAGGFPVEFPVFSNGESLLRPTAMFTRNLASMDVEESIRGNPLDAVVLLV 122

Query: 127 GCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEITIHD 186
           GCDKTTPALLMGAASCD+P IVV+GGPMLNGKH+GRDIGSGTVVWQLSEQVKAG+I+IH+
Sbjct: 123 GCDKTTPALLMGAASCDIPTIVVSGGPMLNGKHEGRDIGSGTVVWQLSEQVKAGKISIHE 182

Query: 187 FMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIV 246
           FM+AEAGMSRSAGTCNTMGTASTMACMAE+LG SLPHNAAIPAVDARRYVLAHLSG RIV
Sbjct: 183 FMSAEAGMSRSAGTCNTMGTASTMACMAEALGTSLPHNAAIPAVDARRYVLAHLSGNRIV 242

Query: 247 DMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRG 306
           +MV E L LSK+LTR+AFENAIRTNAAIGGSTNAVIHLKAIAGRIGV+L+L+DW+RIGRG
Sbjct: 243 EMVEEDLRLSKVLTRQAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVELDLDDWSRIGRG 302

Query: 307 TPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIY 366
           TPT+VDLQPSGR+LMEEFYYAGGLPAVLRRLGEAD LPH++ALTVNG+T+WDNVK+AP+Y
Sbjct: 303 TPTLVDLQPSGRFLMEEFYYAGGLPAVLRRLGEADRLPHREALTVNGRTLWDNVKEAPLY 362

Query: 367 NDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKER 426
           NDEV+RPL +PL EDGG+C+LRGNLAPRGAVLKPSAA+PELM HRGRAVVFEDF+ YK R
Sbjct: 363 NDEVIRPLERPLREDGGMCVLRGNLAPRGAVLKPSAASPELMIHRGRAVVFEDFDDYKAR 422

Query: 427 INDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAY 486
           INDPDL+VDAS VLVMKN GP+GY GMAEVGNMGLP K+L  GV DMVRISDARMSGTAY
Sbjct: 423 INDPDLEVDASSVLVMKNCGPRGYHGMAEVGNMGLPAKLLEQGVTDMVRISDARMSGTAY 482

Query: 487 GTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVLAERKP 546
           GTV+LHVAPE+AAGGPL  V+DGD+IELD  AG L L++ + E++ RL  R      R+ 
Sbjct: 483 GTVVLHVAPESAAGGPLAAVRDGDWIELDCDAGTLHLEVDDAELEARLAERDPTAESRRI 542

Query: 547 EMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585
              GGY+ LY++RVLQADEGCDFDFL G RGA VP+HSH
Sbjct: 543 ASDGGYRQLYIERVLQADEGCDFDFLRGSRGADVPRHSH 581


Lambda     K      H
   0.319    0.136    0.409 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1272
Number of extensions: 42
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 585
Length of database: 581
Length adjustment: 36
Effective length of query: 549
Effective length of database: 545
Effective search space:   299205
Effective search space used:   299205
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources