Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_086511017.1 BZY95_RS16650 dihydroxy-acid dehydratase
Query= reanno::BFirm:BPHYT_RS19730 (577 letters) >NCBI__GCF_002151265.1:WP_086511017.1 Length = 599 Score = 461 bits (1187), Expect = e-134 Identities = 256/551 (46%), Positives = 345/551 (62%), Gaps = 16/551 (2%) Query: 1 MADSNQTKKPLRSQAWFGLKDRDGF--LHRSWMKNQGIPHDEF-DGRPVIGICNTWSELT 57 M D T + LRS+ WF + G L N GI +E G+P+IGIC + S+LT Sbjct: 1 MTDRKITPEQLRSRWWFDNPESPGTTALCIERYMNYGITLEELAGGKPIIGICQSGSDLT 60 Query: 58 PCNAHFRELAEYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNP 117 PCN H EL + VK+G+ AGG+P EFP+ + E RPTA L RNLA + + E + G P Sbjct: 61 PCNRHHIELVKRVKEGIRAAGGVPFEFPLHPIHENARRPTAALDRNLAYLGLVEVLHGYP 120 Query: 118 MDGVILLVGCDKTTPALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVR 177 +DGV+L GCDKTTPA LM AA+ N+PA+ +SGGPMLNG +GSGT +W++ + + Sbjct: 121 LDGVVLTTGCDKTTPASLMAAATVNIPAIVLSGGPMLNGWRGPDRVGSGTIIWELRKRLA 180 Query: 178 AGTMTQEEFTEAESCMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLA 237 AG + EF + S GHC TMGTASTM SM E+LGM LP +A IPA R ++A Sbjct: 181 AGDIDYAEFLARATDSAPSVGHCNTMGTASTMNSMAEALGMSLPGSAMIPAPYKERAMVA 240 Query: 238 HLAGRRIVDMVREDLTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLE 297 + G RIVDMV +DL ILTRQAFENAI +A+GGS+NA +H+ A+A+ GVEL+ + Sbjct: 241 YDTGARIVDMVWQDLRPSDILTRQAFENAIVVCSALGGSSNAPIHINAIARHSGVELTND 300 Query: 298 DWE-LGSNVPCLVNLQPSGEYLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDN 356 DW+ LG +P L N+ P+G YL E+FY AGG+PAV+ +L G LH EALTVNG+TL DN Sbjct: 301 DWQALGHAIPLLANVMPAGAYLSEEFYRAGGVPAVVNELLGAGKLHGEALTVNGRTLADN 360 Query: 357 VRNAANYDEKVITTFAEPFKPKAGIAVLKGNLAPNGAVIKPSAATASLLKH--------- 407 D +VI + P +AG LKGNL + A++K S +A Sbjct: 361 CAGRETQDPEVIRRYDNPLVEQAGFLNLKGNLF-DSALMKTSVISADFRARFLSNPDDPN 419 Query: 408 --RGRAVVFENIEELHAKIDDESLDIDEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQK 465 G+ VVF+ E+ HA+IDD +LDIDEH I+V++GAGP G+PG AEV NM P+ ++++ Sbjct: 420 AFEGKVVVFDGSEDYHARIDDPALDIDEHTILVMRGAGPVGHPGGAEVVNMQPPEALIKR 479 Query: 466 GITDMVRISDGRMSGTAYGAVVLHVSPEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDE 525 GI + + DGR SGT+ +L+ +PEAA GG LA ++ GD + +D+ + L V D Sbjct: 480 GIESLPCLGDGRQSGTSGSPSILNAAPEAATGGGLALLEDGDRLRVDLNRGEVRLLVDDA 539 Query: 526 ELARRRAAWQA 536 EL RR +A Sbjct: 540 ELQARRERLEA 550 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 969 Number of extensions: 55 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 577 Length of database: 599 Length adjustment: 37 Effective length of query: 540 Effective length of database: 562 Effective search space: 303480 Effective search space used: 303480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory