Align 2-methylcitrate dehydratase (EC 4.2.1.79) (characterized)
to candidate WP_086511662.1 BZY95_RS20075 2-methylcitrate dehydratase
Query= BRENDA::P77243 (483 letters) >NCBI__GCF_002151265.1:WP_086511662.1 Length = 494 Score = 608 bits (1567), Expect = e-178 Identities = 304/490 (62%), Positives = 383/490 (78%), Gaps = 16/490 (3%) Query: 7 NIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDTLGCGLEALEYPACKKLLGPIVP 66 N+RP++D E+ I DYV++Y I+S+ A +TA +CL+DTLGCGL AL +P C K LGP+V Sbjct: 8 NVRPDYDPELQKIADYVLDYRIASEEALETARHCLVDTLGCGLLALRFPECIKHLGPLVE 67 Query: 67 GTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFNDTWLAAEWGHPSDNLGGILATADW 126 GTVVPNG RVPGT F+LDPV+AA++IG ++RWLD+NDTWLAAEWGHPSDNLG ILA AD Sbjct: 68 GTVVPNGARVPGTAFRLDPVKAAWDIGCIVRWLDYNDTWLAAEWGHPSDNLGAILAVADH 127 Query: 127 LSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIALENSFNRVGLDHVLLVKVASTAVVAEM 186 LS+ VA G+APLTM+ VL AMIKAHEIQG +ALENSFNRVGLDHV+LVKVASTAV A + Sbjct: 128 LSQKRVAQGEAPLTMRDVLEAMIKAHEIQGVLALENSFNRVGLDHVVLVKVASTAVAAHL 187 Query: 187 LGLTREEILNAVSLAWVDGQSLRTYRHAPNTGTRKSWAAGDATSRAVRLALMAKTGEMGY 246 +G +RE++L+A+S AWVDGQSLRTYRHAPN G+RKSWAAGDATSR VRLA +A GEMG Sbjct: 188 MGASREQLLSALSHAWVDGQSLRTYRHAPNAGSRKSWAAGDATSRGVRLADIALRGEMGV 247 Query: 247 PSALTAPVWGFYDVSF-----------KGE-SFRFQRPYGSYVMENVLFKISFPAEFHSQ 294 P L+AP WGFYDV F +GE FRFQR +G+YVMEN+LFKI+FPAEFH+Q Sbjct: 248 PGVLSAPQWGFYDVLFSKTNKDQAIKPEGERKFRFQREFGTYVMENILFKIAFPAEFHAQ 307 Query: 295 TAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDKKGPLNNPADRDHCIQYMVAI 354 TA EAA+ L+ Q++ A+I++V I THE+ IRII K+G L NPADRDHC+QYMVA+ Sbjct: 308 TACEAAVRLHPQVK---DRLAEIQRVVITTHESAIRIISKEGELANPADRDHCLQYMVAV 364 Query: 355 PLLFGRLTAADYEDNV-AQDKRIDALREKINCFEDPAFTADYHDPEKRAIANAITLEFTD 413 PL+FG LTA YED+ Q ID LR+K+ E+P +T +Y +KR+IANAI + F+D Sbjct: 365 PLIFGDLTAEHYEDDFHRQHPLIDVLRDKMEVVEEPRYTQEYLAADKRSIANAIQVFFSD 424 Query: 414 GTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQFPTRQQQRILEVSLDRARLEQMP 473 GT E + VEYPIGH RRR +GIP L +KF+ +LA +FP + +RI+ + D+A+LE MP Sbjct: 425 GTATECIEVEYPIGHRRRRDEGIPILEEKFRRHLATRFPAGRCERIIALCQDQAQLEAMP 484 Query: 474 VNEYLDLYVI 483 V+ ++DL+VI Sbjct: 485 VHRFVDLFVI 494 Lambda K H 0.321 0.136 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 737 Number of extensions: 26 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 483 Length of database: 494 Length adjustment: 34 Effective length of query: 449 Effective length of database: 460 Effective search space: 206540 Effective search space used: 206540 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory