Alignments for a candidate for fruI in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for fruI in Halomonas desiderata SP1

Align Phosphoenolpyruvate--protein phosphotransferase (EC 2.7.3.9) (characterized)
to candidate WP_086509481.1 BZY95_RS08245 phosphoenolpyruvate-protein phosphotransferase PtsP

Query= reanno::WCS417:GFF780
         (952 letters)



>NCBI__GCF_002151265.1:WP_086509481.1
          Length = 753

 Score =  347 bits (890), Expect = 2e-99
 Identities = 228/583 (39%), Positives = 332/583 (56%), Gaps = 38/583 (6%)

Query: 392 LSAP-LAGSQVQ--AIAAAPGIAIGPAHIQVLQVFDYPLRGESCAIERE----RLHSALA 444
           LS P L G Q     + A+PG+AIG   + V  + D     +    + E    RL  A+ 
Sbjct: 163 LSKPTLPGGQAMFTGVPASPGMAIGEV-VVVAPLADLDSVPDLIPTDVEHEIARLKEAIE 221

Query: 445 DVRRDIQGLIERSQSKAIRE---IFVTHQEMLDDPELTDEVDTRLKQGESAEAAWMSVIE 501
            VR +I+   ER  ++   +   +F  +Q+ML +  L+ EV+ R+++G+ A  A   V+ 
Sbjct: 222 RVREEIRAAGERLANRISAQELALFEVYQQMLSEAALSQEVEKRIREGQWAPGALADVVR 281

Query: 502 AAAKQQESLQDALLAERAADLRDIGRRVLAQLCGVETSQEPS---EPYILVMDEVGPSDV 558
              +  E + D  L ERAAD+RD+GRRVLA L   ET Q P+   E  ILV DE+  + +
Sbjct: 282 RHVQYLERVDDDYLRERAADVRDLGRRVLAHL-QEETPQTPANFPESTILVGDEISVAML 340

Query: 559 ARLDPARVAGILTARGGATAHSAIVARALGIPALVGAGPAVLLLAAGTPLLLDGQRGRLH 618
             +   R+AG+++ RG +T+H AIVARA+GIP + G     L   +G  ++LDG RGRL 
Sbjct: 341 GEVPRERLAGLVSVRGSSTSHVAIVARAMGIPTVSGMVDLPLPRLSGAEVVLDGHRGRLF 400

Query: 619 VDADAATLQRATVERDTREQRLQAASAQ-----RHE---PALTRDGHAVEVFANIGESAG 670
           V        R + E   R   L A  A       HE   P+ T DGH + +  N G +  
Sbjct: 401 V--------RPSDELKARYSSLIAEEAALTEVLEHEQDLPSETPDGHVMPLMVNTGLAID 452

Query: 671 VASAVEQGAEGIGLLRTELIFMAHPQAPDEATQEAEYRRVLDGLAGRPLVVRTLDVGGDK 730
            A+++++   G+GL RTE+ FM   + P E  Q   YR  L+  A  P+V+RTLD+GGDK
Sbjct: 453 TAASLKKRISGVGLYRTEVPFMIAERFPGEQEQARLYRDQLESFAPLPVVMRTLDIGGDK 512

Query: 731 PLPYWPIAEEENPFLGVRGIRLTLQRPQIMEAQLRALLRSADNRP-LRIMFPMVGSVDEW 789
            LPY+PI EE NPFLG RGIR+TL  P+++  QLRA+LR+A     L+I+ PMV +VDE 
Sbjct: 513 DLPYFPI-EEANPFLGWRGIRVTLDHPEVLMVQLRAMLRAAQGLDNLQILLPMVTNVDEV 571

Query: 790 RAARDMTERLRLEIPVADL-----QLGIMIEVPSAALLAPVLAKEVDFFSVGTNDLTQYT 844
             A  + +R   E+    +     ++G+M+EVP+       LA+ VDFFSVG+NDLTQY 
Sbjct: 572 DDALRLLDRAIRELGEEGVVSPRPRVGVMLEVPATLYQLDALAERVDFFSVGSNDLTQYL 631

Query: 845 LAIDRGHPTLSAQADGLHPAVLQLIDITVRAAHAHGKWVGVCGELAADPLAVPVLVGLGV 904
           LA+DR +P +++  D  HPAVL  +      +    K + VCGELA DP    +L+G+G 
Sbjct: 632 LAVDRNNPRVASLYDSCHPAVLCAMARLAEESRRLKKPISVCGELAGDPAGALLLMGMGF 691

Query: 905 DELSVSARSIPEVKARVREFSLSEAQGLAQKALAVGSPAEVRA 947
           D LS++A S+P V+A +R  S+  A+ L Q+ LA+ +P  VR+
Sbjct: 692 DSLSMNAPSLPRVRAAIRRVSIDAAKALVQETLALNTPVAVRS 734


Lambda     K      H
   0.318    0.135    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1470
Number of extensions: 83
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 952
Length of database: 753
Length adjustment: 42
Effective length of query: 910
Effective length of database: 711
Effective search space:   647010
Effective search space used:   647010
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources