Alignments for a candidate for glpD in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpD in Halomonas desiderata SP1

Align glycerol-3-phosphate dehydrogenase; EC 1.1.5.3 (characterized)
to candidate WP_086509596.1 BZY95_RS08920 glycerol-3-phosphate dehydrogenase

Query= CharProtDB::CH_091834
         (512 letters)



>NCBI__GCF_002151265.1:WP_086509596.1
          Length = 500

 Score =  611 bits (1575), Expect = e-179
 Identities = 309/489 (63%), Positives = 367/489 (75%), Gaps = 4/489 (0%)

Query: 16  DVAVVGGGINGVGIAADAAGRGLSVFLCEQHDLAQHTSSASSKLIHGGLRYLEHYEFRLV 75
           D+ ++GGGING GIA DAAGRGLSV LCEQ DLA  TSSASSKLIHGGLRYLEH+EFRLV
Sbjct: 10  DLFIIGGGINGTGIANDAAGRGLSVSLCEQGDLAGATSSASSKLIHGGLRYLEHHEFRLV 69

Query: 76  REALAEREVLLAKAPHIVKPLRFVLPHRPHLRPAWMIRAGLFLYDHLGKREKLPASRGLR 135
            EAL EREVLL KAPHI+ PLRF+LPHR HLRPAWMIRAGLFLYDHLGKR+ LP SR +R
Sbjct: 70  GEALREREVLLRKAPHIIWPLRFILPHRSHLRPAWMIRAGLFLYDHLGKRKMLPGSRSVR 129

Query: 136 FTGSSPLKAEIRRGFEYSDCAVDDARLVVLNAISAREHGAHVHTRTRCVSARRSKGLWHL 195
           F   SPL+  I+RGFEYSDC VDDARLVVLNA+ ARE GA +  RTRC+ A+   G+W +
Sbjct: 130 FAADSPLQEGIKRGFEYSDCWVDDARLVVLNALQAREGGAEILVRTRCIGAKEENGIWRI 189

Query: 196 HLER-SDGSLYSIRARALVNAAGPWVARFIQDDLKQKSPYGIRLIQGSHIIVPKLYEGEH 254
            LE    G  +  +AR LVNAAGPWV R +++   + S Y IR+IQGSH+IVP+L   + 
Sbjct: 190 ELEDVRSGERFERQARTLVNAAGPWVERVVREQAARSSRYSIRMIQGSHLIVPRLNGDDR 249

Query: 255 AYILQNEDRRIVFAIPYLDRFTMIGTTDREYQGDPAKVAISEEETAYLLQVVNAHFKQQL 314
           AYILQN DRRIVF +PY D F++IGTTDR YQGDP + AISEEE  YLL VVNAHF++QL
Sbjct: 250 AYILQNSDRRIVFVLPYEDDFSLIGTTDRRYQGDPTQAAISEEEIDYLLGVVNAHFRRQL 309

Query: 315 AAADILHSFAGVRPLCDDESDEPSAITRDYTLSLSAGNGEPPLLSVFGGKLTTYRKLAES 374
             +D++ SF+GVRPLCDDES EPSA+TRDYTL+    +   PLLSVFGGK+TTYRKLAE+
Sbjct: 310 QRSDVISSFSGVRPLCDDESAEPSAMTRDYTLAFD--DQGAPLLSVFGGKITTYRKLAEA 367

Query: 375 ALTQLQPFFANLGPAWTAKAPLPGGEQMQSVEALTEQLANRYAWLDRELALRWARTYGTR 434
           AL QL+P    +GP+WTA+A LPGGE + + E    +L + Y WL    A R+AR+YG+ 
Sbjct: 368 ALKQLKPVLPAMGPSWTAEAALPGGE-IGNQENFMARLLHDYPWLGEARARRFARSYGSL 426

Query: 435 VWRLLDGVNGEADLGEHLGGGLYAREVDYLCKHEWAQDAEDILWRRSKLGLFLSPSQQVR 494
             R L G     DLGEH G GL A E DYL  HEWA   EDILWRR+KLGL L+P Q  R
Sbjct: 427 CLRFLAGTQCAEDLGEHFGAGLTAAEADYLVDHEWATTVEDILWRRTKLGLRLTPRQVER 486

Query: 495 LGQYLQSEH 503
           L  ++++ H
Sbjct: 487 LQAHVEAHH 495


Lambda     K      H
   0.321    0.136    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 802
Number of extensions: 28
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 512
Length of database: 500
Length adjustment: 34
Effective length of query: 478
Effective length of database: 466
Effective search space:   222748
Effective search space used:   222748
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources