Align ABC transporter for Glycerol, periplasmic substrate-binding component (characterized)
to candidate WP_086511676.1 BZY95_RS20120 carbohydrate ABC transporter substrate-binding protein
Query= reanno::acidovorax_3H11:Ac3H11_796 (577 letters) >NCBI__GCF_002151265.1:WP_086511676.1 Length = 579 Score = 685 bits (1768), Expect = 0.0 Identities = 327/579 (56%), Positives = 422/579 (72%), Gaps = 7/579 (1%) Query: 2 KMQFKAIA--FAAAALAMGQAAWAGEAEAKKWIDSEFQPSTLNKDQQMAEMKWFIDAAKK 59 ++Q AIA A+ A+ A A++ +D+ FQ STL++++Q+ E+ WF AA+ Sbjct: 5 RIQLTAIAGSLLLASAALHAQTDDARAIAERLVDTHFQNSTLSREEQIEELLWFAQAAEP 64 Query: 60 LQAKGVKEISVVSETITTHEYESKTLAKAFEEITGIKVKHDLIQEGDVVEKLQTSMQSGK 119 + +I+ V+E +TTH +E LA AFEE+TGI+V H++I EGD+V+ +QT MQ+G+ Sbjct: 65 FRGM---QINTVAEGLTTHVWERDVLAPAFEELTGIRVTHNIIGEGDLVDNMQTQMQTGR 121 Query: 120 SIYDGWISDSDLIGTHYRYGKIMNLTDYMGGAGKEWTNPGIDLKDYIGTKFTTGPDGKLY 179 +IYDG+I+DSD IGTH RYG +NL+ M ++T P +DL D+IG ++TTGPDG +Y Sbjct: 122 NIYDGYINDSDAIGTHIRYGTTINLSQAMENEWADFTLPTLDLDDFIGIQYTTGPDGSIY 181 Query: 180 QLPDQQFANLYWFRADLFDRKDIKDKFKAKYGYDLGVPLNWSAYEDIAEFFTNDVKNIDG 239 QLP QQFANLYWFR D F R+D++++F+ YGYDLGVP NW+AYE+IAEFFT V+ IDG Sbjct: 182 QLPAQQFANLYWFRYDWFQREDLQEQFREIYGYDLGVPTNWTAYENIAEFFTEHVREIDG 241 Query: 240 KPIYGHMDYGKKDPSLGWRFTDAWLSMAGTADIGAPNGLPIDEWGIRVADDKCTPVGASV 299 +YGHMDYG++DPSLGWRF D+WLSMAG G P G P+D+WGIRV D++ PVGASV Sbjct: 242 VRVYGHMDYGRRDPSLGWRFHDSWLSMAGMGSPGIPFGNPVDDWGIRV-DEESRPVGASV 300 Query: 300 ARGGATNSPAAVYALTKYVDWMKKYAPKEATGMTFGEAGPVPAQGQIAQQIFWYTAFTAD 359 +RGG TN+PA+V+A+ K VDW++ YAP EA GMTFGEAGPVPAQG IAQQIFWYTAFTAD Sbjct: 301 SRGGGTNAPASVFAMQKAVDWLRDYAPPEAQGMTFGEAGPVPAQGNIAQQIFWYTAFTAD 360 Query: 360 MTKPGLPVVNADGTPKWRMAPGPNGPYWKQGMQNGYQDVGSWTFFKDHDANKTAAAWLYA 419 M P + V + +G P WRMAP P GPYW++GM+ GYQDVGSWTFF ++ AAWL+ Sbjct: 361 MIDPSVAVTDDEGNPLWRMAPSPVGPYWEEGMKVGYQDVGSWTFFDSTPEDRRTAAWLFG 420 Query: 420 QFVTAKTTSLKKTMVGLTPIRESDIQSKAMTDMAPKLGGLVEFYRSPARVAWSPTGTNVP 479 QF AKT SL+K M GLTPIRESDI S MT+MAP+LGGLVEFYRSP W+PTGTNVP Sbjct: 421 QFTVAKTVSLEKLMAGLTPIRESDIFSDQMTEMAPRLGGLVEFYRSPNESMWTPTGTNVP 480 Query: 480 DYPKLAQLWWKNVAQAVTGEKTPQGAMDTLADEMDQVMARLERAGMAHC-APKLNAKSDP 538 DYP++A LWW+N+A ++GE +PQ +D LA MD +MARL RA + P LN + DP Sbjct: 481 DYPRMAPLWWQNLAPVISGEISPQEGLDNLAGAMDNIMARLARANVFEAYPPVLNEERDP 540 Query: 539 NKWLSDKQAPWKKLANEKPKGETIAYGTLLQAWKDGKTR 577 WLS AP KL +E P+G TI Y +++ W TR Sbjct: 541 EYWLSQPGAPKAKLDDEMPQGTTIPYDQMMEEWMAAGTR 579 Lambda K H 0.315 0.132 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1156 Number of extensions: 49 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 577 Length of database: 579 Length adjustment: 36 Effective length of query: 541 Effective length of database: 543 Effective search space: 293763 Effective search space used: 293763 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory