Align Histidine ammonia-lyase (EC 4.3.1.3) (characterized)
to candidate WP_086509677.1 BZY95_RS09375 histidine ammonia-lyase
Query= reanno::pseudo5_N2C3_1:AO356_09605 (507 letters) >NCBI__GCF_002151265.1:WP_086509677.1 Length = 527 Score = 575 bits (1482), Expect = e-168 Identities = 295/492 (59%), Positives = 368/492 (74%) Query: 4 AEKIVITGAPLRWQDVVAVARFGAELELSTQTWARIDNAQAIVQRIVESGERAYGVNTGL 63 AE+IV+ AP+ WQ VV VAR GA L L+ W RI A+ V++I S YG+NTGL Sbjct: 5 AERIVLGAAPVEWQQVVRVARHGALLSLTKAAWKRIAAARQAVEKIAASTVPHYGINTGL 64 Query: 64 GALCNVSLKDEQLSQLSRNTLLSHACGVGAPLTDEQTRAILCAAILNYSQGKSGIHRRVV 123 GALCNV+L+ ++L +LS +TL+SHACGVGAPL EQ RAI+C A++NYS G SGI VV Sbjct: 65 GALCNVALERDELQRLSHHTLMSHACGVGAPLRTEQVRAIICCAVINYSHGYSGISPAVV 124 Query: 124 EALLALLNRGITPQVPSQGSVGYLTHMAHIGIALLGVGQVSYRGQIVPAQQALVAEGLQP 183 + LL LLN +TP VPS+GSVGYLTHMAHIG+AL+G G+V + GQ +PA+QAL A G+ P Sbjct: 125 KGLLHLLNEQVTPIVPSRGSVGYLTHMAHIGLALIGHGEVEFDGQRMPARQALDAIGMPP 184 Query: 184 VQLGAKDGLCLVNGTPCMTGLSCLALADATRLVQWADVIGAMSFEAQRGQIAAFDAEIIA 243 + LG KDGL LVNGTP MTGL+CLAL D RL WAD++GAMSFEA GQ A ++ + Sbjct: 185 LTLGPKDGLSLVNGTPAMTGLACLALEDVARLAAWADIVGAMSFEALGGQWDALLPQVTS 244 Query: 244 LKPHPGMQQVGVNLRALLDGSEVIAASRGIRTQDALSIRSIPQVHGAARDQLAHARQQIE 303 LK H G+Q G NLR LL GS +A +G QDALS+RSIPQVHGA RDQ AHA +QI+ Sbjct: 245 LKRHQGVQHTGDNLRRLLQGSRRLARCQGQHLQDALSLRSIPQVHGACRDQFAHAARQID 304 Query: 304 TELNAVTDNPLLLGTPENFRVMSQANPHGQSVALAADLLAIAMAEIGSIAERRLDRLINP 363 EL++ TDNPL++ T E++R++S ANPHG SVA+A DLLAIA+ E SI+ERR RL+ P Sbjct: 305 HELDSATDNPLVVATEESYRIISNANPHGASVAMACDLLAIAVCEWSSISERRTYRLVTP 364 Query: 364 HVSGLPAFLVANPGVNSGMMIVQYVAASLCAENKQLAQPAVLDNFVTSGLQEDHLSMGTN 423 S LP FL + GV SGMMI QY AASL A+NK+LAQPAV+DNF+TSGLQEDHLS+G + Sbjct: 365 QASRLPPFLSNDVGVKSGMMIAQYTAASLVADNKRLAQPAVVDNFLTSGLQEDHLSLGES 424 Query: 424 AALKLHRALENCTQILAIEYLLAAQAFEFLKAQRFGAGTDIAWKLLRERVPPYDQDRWLA 483 AALKL +AL+N Q+LAIEYLLAAQAF+ + Q F GT++AWK LRE +P YD++ L Sbjct: 425 AALKLDQALDNAFQVLAIEYLLAAQAFDLIDDQNFAPGTELAWKALREVIPFYDEEHPLH 484 Query: 484 PDIASAAGLLKD 495 D+ +A LL+D Sbjct: 485 QDLQTARHLLRD 496 Lambda K H 0.320 0.133 0.385 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 758 Number of extensions: 23 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 507 Length of database: 527 Length adjustment: 35 Effective length of query: 472 Effective length of database: 492 Effective search space: 232224 Effective search space used: 232224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory