Align short-chain acyl-CoA dehydrogenase monomer (EC 1.3.8.1) (characterized)
to candidate WP_086509764.1 BZY95_RS09860 acyl-CoA dehydrogenase
Query= metacyc::MONOMER-17424 (375 letters) >NCBI__GCF_002151265.1:WP_086509764.1 Length = 383 Score = 301 bits (772), Expect = 2e-86 Identities = 164/369 (44%), Positives = 231/369 (62%), Gaps = 2/369 (0%) Query: 9 QIADAVRAFAQERLKPFAEQWDKDHRFPKEAIDEMAELGLFGMLVPEQWGGSDTGYVAYA 68 Q+ D + F +ERL P + ++ P E ++EM E+GLFG+ +PE++GG A Sbjct: 10 QLIDTISRFVRERLIPNEARLAEEDAVPAELLEEMKEMGLFGLSIPEEYGGLGLTMEEEA 69 Query: 69 MALEEIAAGDGACSTIMSVHNSVGCVPILRFGNEQQKEQFLTPLATGAMLGAFALTEPQA 128 + EI A +I +N +G IL G +QK +++ LATG +L +F LTEP A Sbjct: 70 LVAMEIGKTSPAFRSIFGTNNGIGAQGILIDGTPEQKAKYVPRLATGELLSSFCLTEPDA 129 Query: 129 GSDASSLKTRARLEGDHYVLNGSKQFITSGQNAGVVIVFAVTDP-EAGKRGISAFIVPTD 187 GSDA+SL+T A +GDHYVLNG+K+FIT+G A V V A TDP G GI+AFIV D Sbjct: 130 GSDAASLRTTAVRDGDHYVLNGTKRFITNGPEADVFTVMARTDPGNKGAGGITAFIVEGD 189 Query: 188 SPGYQVARVEDKLGQHASDTCQIVFDNVQVPVANRLGA-EGEGYKIALANLEGGRIGIAS 246 +PG + + K+GQ + TC I+F++ +VP N +G EG+G+K A+ L+ GR+ I++ Sbjct: 190 TPGLKRGPADSKMGQKGAHTCDIIFEDCRVPAENIIGGVEGKGFKTAMKVLDRGRLHISA 249 Query: 247 QAVGMARAAFEVARDYANERQSFGKPLIEHQAVAFRLADMATKISVARQMVLHAAALRDA 306 VG+A E + +YA ER+ FG PL EHQ V LAD T+ R MVL AA +DA Sbjct: 250 VCVGVAERLVEESLNYAIERKQFGVPLAEHQLVQAMLADSKTEAYAGRTMVLDAARRKDA 309 Query: 307 GRPALVEASMAKLFASEMAEKVCSDALQTLGGYGYLSDFPLERIYRDVRVCQIYEGTSDI 366 G A AS KLF +EM +V A+Q GG GY++++ +ER YRDVR+ +IYEGT+ I Sbjct: 310 GENASTLASCCKLFCAEMVGRVADRAVQVHGGAGYMAEYAVERFYRDVRLFRIYEGTTQI 369 Query: 367 QRMVIARNL 375 Q++VIARN+ Sbjct: 370 QQVVIARNM 378 Lambda K H 0.319 0.134 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 354 Number of extensions: 21 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 375 Length of database: 383 Length adjustment: 30 Effective length of query: 345 Effective length of database: 353 Effective search space: 121785 Effective search space used: 121785 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory