Alignments for a candidate for susB in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for susB in Halomonas desiderata SP1

Align α-glucosidase (AglA;HaG) (EC 3.2.1.20) (characterized)
to candidate WP_086510096.1 BZY95_RS11675 DUF3459 domain-containing protein

Query= CAZy::BAL49684.1
         (538 letters)



>NCBI__GCF_002151265.1:WP_086510096.1
          Length = 536

 Score =  877 bits (2267), Expect = 0.0
 Identities = 405/536 (75%), Positives = 456/536 (85%), Gaps = 1/536 (0%)

Query: 1   MQDNMMWWRGGVIYQIYPRSFLDSRGDGVGDLNGITEKLDYVASLNVDGIWLSPFFTSPM 60
           MQDN  WWRGGVIYQIYPRSF+DS GDGVGDL G+TE+LDYVASL VDGIWLSPFFTSPM
Sbjct: 1   MQDNTFWWRGGVIYQIYPRSFMDSNGDGVGDLPGVTERLDYVASLGVDGIWLSPFFTSPM 60

Query: 61  LDFGYDVSDYRDVDPMFGTLEDFKALLEKAHSLGLKVMIDQVISHTSDQHPWFQESRQNR 120
            DFGYD+S+YRDVDP+FGTL DFKALLE+AH+LGLKV+IDQVISHTSDQHPWFQESR NR
Sbjct: 61  RDFGYDISNYRDVDPLFGTLADFKALLERAHALGLKVIIDQVISHTSDQHPWFQESRMNR 120

Query: 121 TNPKADWFVWADPKPDGTPPNNWLSIFGGSAWTFDSRRQQYYLHNFLTSQPDVNFHHPEA 180
            NP+ADW+VWADP PDGT PNNWLSIFGG AWTFDSRRQQYYLHNFL+SQPD+NFH+PE 
Sbjct: 121 DNPRADWYVWADPNPDGTAPNNWLSIFGGPAWTFDSRRQQYYLHNFLSSQPDLNFHNPEV 180

Query: 181 RQAQLDNMRFWLDLGVDGFRLDTVNFYFHDAELRDNPPVPKGEAKTLGAPEANPYTWQRH 240
           R AQLDNMRFWL+LGVDGFRLDTVNFYFHD  LR NP +     +TLGAP+ NPYTWQRH
Sbjct: 181 RAAQLDNMRFWLELGVDGFRLDTVNFYFHDQHLRSNPALTIDATRTLGAPKENPYTWQRH 240

Query: 241 VYDLSRPENLDFLKDLRALMDEYPGTTTVGEIGDDNPLERMAEYTAGGDKLHMAYTFDLL 300
           +YD+SRPEN++FL++LRALMDEYPGTTTVGEIGDD PLERMAEYT+GGDKLHMAYTFDLL
Sbjct: 241 IYDISRPENVEFLRELRALMDEYPGTTTVGEIGDDTPLERMAEYTSGGDKLHMAYTFDLL 300

Query: 301 NMPHSASYLREVIERFQRLAGDAWPCWATSNHDVVRSATRWGADEDPHAYPKVMLAVLFS 360
           N P S  Y+R V+ERFQ+ AGDAWPCWA SNHDVVRSATRWG++E   AYP+V LA+LFS
Sbjct: 301 NAPRSPDYIRGVLERFQQFAGDAWPCWALSNHDVVRSATRWGSEECAFAYPRVALALLFS 360

Query: 361 LRGSVCLYQGEELGLPEADVPFERIQDPYGKVLWPEFKGRDGCRTPMPWTDGEQGGFSPV 420
           LRGSVCLYQGEELGLPEA+VPFERIQDPYG  LWP+FKGRDGCRTPMPW D    GFS V
Sbjct: 361 LRGSVCLYQGEELGLPEAEVPFERIQDPYGLPLWPDFKGRDGCRTPMPWDDTPLAGFSDV 420

Query: 421 EPWLPMEARHLELAVSRQQDDPNATLNTVRALLAFRRSHPALFDGDLSLVDVGDDLLGFT 480
           EPWLP+   HL ++V+RQQD+P++ LN  R LL FR  HPALFDGD++L+D+G+ L+ FT
Sbjct: 421 EPWLPVAEPHLAMSVARQQDEPDSMLNATRRLLRFRHQHPALFDGDMTLLDLGEALVAFT 480

Query: 481 RQKGDETLLCVFNLTGQEQQTTLPVEVASDLPVAHFTATRDGSTLTLPAYQAAFMQ 536
           RQ+G + LLCVFNLTGQ Q  TLP EV   L    F     G  L LP YQAA+ +
Sbjct: 481 RQRGSDRLLCVFNLTGQPQSATLP-EVREALQEHGFQYEVSGGRLHLPPYQAAYFR 535


Lambda     K      H
   0.320    0.138    0.443 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1216
Number of extensions: 48
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 538
Length of database: 536
Length adjustment: 35
Effective length of query: 503
Effective length of database: 501
Effective search space:   252003
Effective search space used:   252003
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources