Alignments for a candidate for mtlE in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for mtlE in Halomonas desiderata SP1

Align SmoE, component of Hexitol (glucitol; mannitol) porter (characterized)
to candidate WP_086511131.1 BZY95_RS17260 sugar ABC transporter substrate-binding protein

Query= TCDB::O30831
         (436 letters)



>NCBI__GCF_002151265.1:WP_086511131.1
          Length = 434

 Score =  525 bits (1351), Expect = e-153
 Identities = 250/421 (59%), Positives = 309/421 (73%), Gaps = 1/421 (0%)

Query: 15  AALSSAAGAETITVATVNNGDMIRMQGLMSEFNAQHPDITVEWVTLEENVLRQKVTTDIA 74
           AA ++AA AETITVATVNN DM+ MQ L S F   HP I V WV LEENVLRQ++TTDIA
Sbjct: 14  AAFATAAQAETITVATVNNNDMVIMQSLTSAFEEAHPGIRVNWVVLEENVLRQRLTTDIA 73

Query: 75  TKGGQFDVLTIGTYEVPIWGKQGWLVSLNDLPPEYDADDILPAIRNGLTVDGELYAAPFY 134
           T GGQFDV+TIGTYE PIW ++GWLV+L+DLP     +D+L  +R+GL+ +G LYA PFY
Sbjct: 74  TDGGQFDVMTIGTYEAPIWAERGWLVALDDLPEASQEEDLLKPVRDGLSHNGSLYALPFY 133

Query: 135 GESSMIMYRKDLMEKAGLTMPDAPTWDFVKEAAQKMTDKDAEVYGICLRGKAGWGENMAF 194
            ESSM+ YR DL E+AG+ MP+ P+W+ V+E A ++ D   ++ GICLRGK GWGENMAF
Sbjct: 134 AESSMLYYRTDLFEEAGIEMPEQPSWEEVREWAAELHDPANQLAGICLRGKPGWGENMAF 193

Query: 195 LSAMANSYGARWFDENWQPQFDGEAWKATLTDYLDMMTNYGPPGASKNGFNENLALFQQG 254
           +S + N++G RWFDE W P+ D  AW+  +  Y+D++ NYGPPGAS NGFNENLALF +G
Sbjct: 194 VSTLVNTFGGRWFDEEWNPELDSPAWQEAIGFYVDLLGNYGPPGASSNGFNENLALFSRG 253

Query: 255 KCGMWIDATVAASFVTNPEESTVADKVGFALAPDTGKGKRANWLGAWNLAIPAGSQKVDA 314
            C MW+DAT AA  + NP ES VAD++GFA AP     K A+WL +W LAIPA S+K +A
Sbjct: 254 NCAMWVDATSAAGKLYNPAESQVADRLGFAPAPVAETPKGAHWLWSWALAIPASSEKQEA 313

Query: 315 AKQFIAWATSKDYAELVASKEGWANVPPGTRTSLYENPEYQK-VPFAKMTLDSINAADPT 373
           A+ F+ WATS++Y ELV   +GW +VPPGTR S Y N  YQ+  PFA   LD+IN+ADPT
Sbjct: 314 AQTFLTWATSQEYIELVGETQGWTSVPPGTRESTYANENYQREAPFAGFVLDAINSADPT 373

Query: 374 HPAVDPVPYVGVQFVAIPEFQGIGTAVGQQFSAALAGSMSAEQALQAAQQFTTREMTRAG 433
              ++P PYVGVQFV IPEFQ IGT VGQ  +AAL G    EQALQ AQ+ T R M RAG
Sbjct: 374 DSTLEPSPYVGVQFVGIPEFQSIGTQVGQTIAAALTGDTGVEQALQNAQRATARTMQRAG 433

Query: 434 Y 434
           Y
Sbjct: 434 Y 434


Lambda     K      H
   0.316    0.131    0.400 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 628
Number of extensions: 28
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 436
Length of database: 434
Length adjustment: 32
Effective length of query: 404
Effective length of database: 402
Effective search space:   162408
Effective search space used:   162408
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources