Alignments for a candidate for paaH in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaH in Halomonas desiderata SP1

Align fatty acid oxidation complex α subunit (EC 1.1.1.35; EC 4.2.1.17) (characterized)
to candidate WP_086510728.1 BZY95_RS15080 fatty acid oxidation complex subunit alpha FadB

Query= metacyc::MONOMER-17591
         (715 letters)



>NCBI__GCF_002151265.1:WP_086510728.1
          Length = 725

 Score =  953 bits (2463), Expect = 0.0
 Identities = 488/719 (67%), Positives = 578/719 (80%), Gaps = 5/719 (0%)

Query: 1   MIYQGKAITVK--PLEGG--IVELNFDLKGESVNKFNRLTLSELRAAVDAIKADASVKGV 56
           MIYQG AITV      GG  +  L FDLK ES+NK +   ++EL  AV AI+A++ +KG+
Sbjct: 1   MIYQGNAITVARGTKNGGDDVAMLTFDLKDESINKLSSAVIAELDEAVQAIRAESGLKGL 60

Query: 57  IVTSGKDVFIVGADITEFVDNFQLPDEELMAGNLEANKIFSDFEDLDVPTVAAINGIALG 116
           ++ S K+ FIVGADITEF   F    E L +  ++ + IF+  EDL  PTV AING+ALG
Sbjct: 61  VIGSAKESFIVGADITEFQTMFGKGAEFLESMLMKVHDIFNAIEDLPFPTVTAINGLALG 120

Query: 117 GGLEMCLAADFRVMSATAKVGLPEVKLGIYPGFGGTVRLPRLIGCDNAVEWIASGKENKA 176
           GG E+ L  DFRVM   AK+GLPE KLGI PG+GG VRLPR+IG DNAVEWIA G EN+A
Sbjct: 121 GGCEVTLTTDFRVMGDKAKIGLPETKLGILPGWGGCVRLPRMIGSDNAVEWIAGGTENRA 180

Query: 177 EDALKVGAVDAVVAPEQLQAAALDLAKRAVAGELDHKARRQPKLEKLKLNAIEQMMAFET 236
           + ALKVGAVDAVV  +QL+AAALD+  RA AGELD++ARR  K   LKLNAIEQMMAFET
Sbjct: 181 DAALKVGAVDAVVPNDQLEAAALDILARANAGELDYQARRAEKTGPLKLNAIEQMMAFET 240

Query: 237 AKGFVAGQAGPNYPAPVEAIKSIQKAANFGRDKALEVEAAGFVKLAKTSVAQSLIGLFLN 296
           AKG+VAG+AGP+YPAP+EAIK+IQK A   R +A  +EA  F KLA T VA +L+GLF+N
Sbjct: 241 AKGYVAGKAGPHYPAPIEAIKAIQKGAGEERARAQAIEAKAFAKLAMTDVAFNLVGLFMN 300

Query: 297 DQELKKKAKKYDEVAKDVKLAAVLGAGIMGGGIAYQSALKGTPILMKDIREEGIQMGLNE 356
           DQ +KKKA K+++ AK V   AVLGAGIMGGGIAYQSA KGTPILMKDI+EE +++GL E
Sbjct: 301 DQVVKKKAGKFEKQAKAVNQTAVLGAGIMGGGIAYQSASKGTPILMKDIKEEAVELGLKE 360

Query: 357 AAKLLGKRVEKGRLTPAKMAEALNGIRPTMSYGDFGNVDIVVEAVVENPKVKQAVLAEVE 416
           + KL  K+VE+G+LT  +MAE L  IRPT+SYGDFG+VD+VVEAVVENPKVK AVL EVE
Sbjct: 361 SRKLFAKQVERGKLTTEQMAEKLTLIRPTLSYGDFGHVDLVVEAVVENPKVKDAVLTEVE 420

Query: 417 GAVKEDAIIASNTSTISISLLAQALKRPENFCGMHFFNPVHMMPLVEVIRGEKTGETAIA 476
           G V ED I+ SNTSTISI+ LAQ LKRPENFCGMHFFNPVH MPLVEVIRGEKTG+ A+A
Sbjct: 421 GLVSEDTILTSNTSTISITRLAQNLKRPENFCGMHFFNPVHRMPLVEVIRGEKTGDAAVA 480

Query: 477 TTVAYAKKMGKSPIVVNDCPGFLVNRVLFPYFGGFAKLLSFGVDFVRIDKVMEKFGWPMG 536
            TVAYA+ MGK+PIVVNDCPGFLVNRVLFPYFGGF+ L+  G DF R+DKVMEKFGWPMG
Sbjct: 481 ATVAYARAMGKTPIVVNDCPGFLVNRVLFPYFGGFSLLVEQGADFQRVDKVMEKFGWPMG 540

Query: 537 PAYLSDVVGIDTGHHGRDVMAEGFPDRMAVEGKTAVDVMYEANRLGQKNGKGFYAYETDK 596
           PAYL DVVG+DT  H  +VMAEGFP+RMA +GKTA+ VMYE  RLGQKN KGFYAYE DK
Sbjct: 541 PAYLLDVVGMDTAVHANEVMAEGFPERMARDGKTAIQVMYENERLGQKNDKGFYAYEEDK 600

Query: 597 RGKPKKVTDPQAYEVLKPIVVEQREVTDEDIVNFMMIPLCLETVRCLEDGIVETAAEADM 656
           +GKPKKV+D +AYE++K +VV ++E +DE+I+  MM+PLCLETVRCLED IVET AEADM
Sbjct: 601 KGKPKKVSDDKAYELVKQVVVSEKEFSDEEIIARMMVPLCLETVRCLEDDIVETPAEADM 660

Query: 657 GLIYGIGFPPFRGGALRYIDSIGVAEFVALADKYA-ELGALYHPTAKLREMAKNGQKFF 714
            LIYGIGFPPFRGGALRYID++GVAEFV LAD  A ELG LY PT KLR+MA++G++F+
Sbjct: 661 ALIYGIGFPPFRGGALRYIDAMGVAEFVKLADGLAEELGPLYAPTDKLRKMAESGERFY 719


Lambda     K      H
   0.318    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1381
Number of extensions: 42
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 715
Length of database: 725
Length adjustment: 40
Effective length of query: 675
Effective length of database: 685
Effective search space:   462375
Effective search space used:   462375
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources