Align oxepin-CoA hydrolase (EC 3.3.2.12) (characterized)
to candidate WP_086509216.1 BZY95_RS06870 phenylacetic acid degradation bifunctional protein PaaZ
Query= BRENDA::P77455 (681 letters) >NCBI__GCF_002151265.1:WP_086509216.1 Length = 680 Score = 724 bits (1870), Expect = 0.0 Identities = 378/680 (55%), Positives = 469/680 (68%), Gaps = 6/680 (0%) Query: 1 MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAARQFAIEKGAPALRAMT 60 M L S+++ W G ++ + AI+GE + +E +D + + G L A+ Sbjct: 1 MPVLKSYVASQWV-GATAAKALPSAINGETIAHTHAETIDFSQVVDYGRHTGVKNLLAVD 59 Query: 61 FIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEGGIGTLFTYASLGSRELPD 120 F +RAA+LKA+A ++ KE Y +S TG TRADSW+DIEGG GTLF YAS+G + LP Sbjct: 60 FQQRAAILKALAIYIQERKEALYEISHHTGCTRADSWIDIEGGFGTLFAYASIGRKGLPS 119 Query: 121 DTLWPEDELIPLSKEGGFAARHLLTSKSGVAVHINAFNFPCWGMLEKLAPTWLGGMPAII 180 + E I L K+ F H+L + GV VHINAFNFP WGMLEK AP +L GMP I+ Sbjct: 120 GNVAHEGPSIQLGKDNHFNGTHILVPRRGVCVHINAFNFPIWGMLEKFAPAFLAGMPCIV 179 Query: 181 KPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDLLDHLDSQDVVTFTGSAATGQML 240 KPATAT+ L +A V+ I +SGL+P GA+ LI G GDLLD L+ QD VTFTGSAAT L Sbjct: 180 KPATATSYLAEAAVRIIDESGLLPAGALQLIIGGTGDLLDRLEEQDFVTFTGSAATALKL 239 Query: 241 RVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEFALFIREVVREMTTKAGQKCTAIRR 300 + PN++A+SIPF EADSLN +L DVTPD EF +F +EVV+EMT KAGQKCTAIRR Sbjct: 240 KSHPNVMARSIPFNAEADSLNSAILAPDVTPDDEEFGIFAKEVVKEMTAKAGQKCTAIRR 299 Query: 301 IIVPQALVNAVSDALVARLQKVVVGDPAQEGVKMGALVNAEQRADVQEKVNILLAA---- 356 ++VP ++A++D + RL K+ GDPAQEGV+MGAL + EQ DV + + LL Sbjct: 300 VLVPAEHLDALADRIKERLGKITFGDPAQEGVRMGALASQEQLRDVTDAIGRLLETSECV 359 Query: 357 -GCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVHATEAFGPVATLMPAQNQRHALQL 415 G RL GAF P LL +P + H EAFGPVATLMP AL + Sbjct: 360 FGKSDRLEPVGKGVENGAFIAPHLLINYRPLQDGGAHDIEAFGPVATLMPYHGIDEALAI 419 Query: 416 ACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQILNEESAKESTGHGSPLPQLVHGGP 475 A G GSL TL T DP IA + I A HGR+QIL+ E++KESTGHGSPLP L HGGP Sbjct: 420 AAKGKGSLVTTLTTRDPAIAAEMIPVLAAQHGRLQILDAEASKESTGHGSPLPMLKHGGP 479 Query: 476 GRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAAISKQWVRGAKVEEDRIHPFRKYFEELQ 535 GRAGGGEELGG+RAV HY+QRTA+QGSPTM+AA+++++VRGAKV E+ +HPFR+YF++LQ Sbjct: 480 GRAGGGEELGGIRAVHHYLQRTAIQGSPTMVAAVTREYVRGAKVIENDVHPFRRYFDDLQ 539 Query: 536 PGDSLLTPRRTMTEADIVNFACLSGDHFYAHMDKIAAAESIFGERVVHGYFVLSAAAGLF 595 G+SLLT RRT+TEADIVNF CLSGDHFY H D IAA ++ FG+R+ HGYFVLSAAAGLF Sbjct: 540 VGESLLTHRRTVTEADIVNFGCLSGDHFYMHFDDIAARKTQFGQRIAHGYFVLSAAAGLF 599 Query: 596 VDAGVGPVIANYGLESLRFIEPVKPGDTIQVRLTCKRKTLKKQRSAEEKPTGVVEWAVEV 655 V G GPV+ANYGL++LRFI PV PGDTI+ RLTCKRK + + S E P GVV W V V Sbjct: 600 VYPGEGPVLANYGLDTLRFITPVAPGDTIRARLTCKRKIDQGRTSPEGYPQGVVMWDVAV 659 Query: 656 FNQHQTPVALYSILTLVARQ 675 NQ VA Y ILTLVA++ Sbjct: 660 TNQKDELVASYDILTLVAKR 679 Lambda K H 0.319 0.134 0.395 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1125 Number of extensions: 46 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 681 Length of database: 680 Length adjustment: 39 Effective length of query: 642 Effective length of database: 641 Effective search space: 411522 Effective search space used: 411522 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory