Align High-affinity branched-chain amino acid transport ATP-binding protein BraG, component of Branched chain amino acid uptake transporter. Transports alanine (characterized)
to candidate WP_086509448.1 BZY95_RS08160 ABC transporter ATP-binding protein
Query= TCDB::P21630 (233 letters) >NCBI__GCF_002151265.1:WP_086509448.1 Length = 226 Score = 184 bits (468), Expect = 9e-52 Identities = 104/232 (44%), Positives = 149/232 (64%), Gaps = 7/232 (3%) Query: 1 MLSFDKVSTYYGKIQALHDVSVEVKKGEIVTLIGANGAGKSTLLMTLCGSPQAASGSIRY 60 ML+ + T+YG QAL V + V+ GE++ L+G NG GK+T + ++ G A SGSI + Sbjct: 1 MLNVTAIETFYGPSQALFGVDLSVRAGEMMALMGRNGMGKTTTIRSIFGLTPARSGSITF 60 Query: 61 EGEELVGLPSSTIMRKSIAVVPEGRRVFSRLTVEENLAMGGFFTDKDDYQVQMDKVLELF 120 E ++L LPS I + + +VPEGRR F L+V ENL + T + Y + +V +LF Sbjct: 61 ESQDLRRLPSYRIAQAGLGLVPEGRRCFPNLSVRENLVV----TARPGYW-SLARVEKLF 115 Query: 121 PRLKERYEQRAGTMSGGEQQMLAIGRALMSKPKLLLLDEPSLGLAPIIIQQIFEIIEQLR 180 PRL ER Q A T+SGGEQQMLAIGRALM+ P+LL+LDE + GLAP+I Q+I+ I +L+ Sbjct: 116 PRLAERRTQMARTLSGGEQQMLAIGRALMTNPRLLVLDEATEGLAPVIRQEIWAAIRELK 175 Query: 181 REGVTVFLVEQNANQALKLADRAYVLENGRIVMHDTGAALLTNPKVRDAYLG 232 +G + +V+++ + L +ADR +LE GR V TG + +RD YLG Sbjct: 176 TQGQSTLIVDKSLAELLPVADRCTILEKGRTVW--TGEPAALDDGLRDRYLG 225 Lambda K H 0.318 0.136 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 138 Number of extensions: 7 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 233 Length of database: 226 Length adjustment: 23 Effective length of query: 210 Effective length of database: 203 Effective search space: 42630 Effective search space used: 42630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory