Alignments for a candidate for ams in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for ams in Halomonas desiderata SP1

Align Alpha-glucosidase; EC 3.2.1.- (characterized, see rationale)
to candidate WP_086510098.1 BZY95_RS11685 DUF3459 domain-containing protein

Query= uniprot:A8LLL3
         (552 letters)



>NCBI__GCF_002151265.1:WP_086510098.1
          Length = 550

 Score =  622 bits (1603), Expect = 0.0
 Identities = 309/552 (55%), Positives = 371/552 (67%), Gaps = 20/552 (3%)

Query: 10  VKSLAADPDWWRGAVIYQIYPRSFQDSNGDGIGDLLGIVERMPYIASLGVDAIWISPFFT 69
           ++ L  DPDWWRGAVIYQIYPRSFQDS G GIGDL G+++R+ YIASL VDAIW++PFFT
Sbjct: 3   IRQLGGDPDWWRGAVIYQIYPRSFQDSRGAGIGDLQGVIDRLDYIASLNVDAIWLAPFFT 62

Query: 70  SPMKDFGYDISDYFDVDPMFGSLADFDALIETAHMYGLRVMIDLVLSHTSDQHPWFEESR 129
           SPMKDFGYD+SDY  VDP FG+L DFD L+E AH  GLRV ID VLSH+SDQHPWF ESR
Sbjct: 63  SPMKDFGYDVSDYRGVDPTFGTLDDFDRLVEAAHARGLRVTIDQVLSHSSDQHPWFAESR 122

Query: 130 SSRDNPKADWYVWADAKPDGTPPNNWLSIFGGSGWHWDARRCQYYLHNFLTSQPDLNFHC 189
           SSRDNPKADWYVWADA+ DG+PP NW +IFGGS W WD RRCQYYLHNFL  QPDLNFH 
Sbjct: 123 SSRDNPKADWYVWADARSDGSPPTNWQAIFGGSAWQWDTRRCQYYLHNFLVEQPDLNFHN 182

Query: 190 ADVQDALLGVGRFWLDRGVDGFRLDTINFYVHDAELRDNPPLPP-EERNSNIAPEVNPYN 248
            +V +A+L   RFWL+RGVDGFRLD +NF  H   L+DNPP     E    + P+ NPY 
Sbjct: 183 PEVVEAILEEVRFWLERGVDGFRLDAVNFCTH-GPLKDNPPREKIVEGFIGVRPD-NPYA 240

Query: 249 HQRHLYSKNQPENLEFLAKFRAMMEEYPAIAAVGEVGDAQYGLEILGQYTRGETGVHMCY 308
            QRHLY K QPENL FL + RA+++EYP+   +GEVGD    L ++ +YT+G   +HMCY
Sbjct: 241 FQRHLYDKTQPENLAFLERLRALLDEYPSTTTIGEVGDDD-SLGVMSEYTQGGKRLHMCY 299

Query: 309 AFEFLAQEKLTAKRVAEVLNKVDEVASDGWACWAFSNHDVMRHVSRWDLTP--GAQRGML 366
           +F  L  +K T   +   L +++    DGW CWA  NHD+ R  +RW++       R  L
Sbjct: 300 SFNLLT-DKATPADLLAPLTEMEARIGDGWPCWAVGNHDITRVATRWNVASDMAKLRLYL 358

Query: 367 TLLMCLRGSVCLYQGEELGLPEAEVAFDDLQDPYGIEFWPEYKGRDGCRTPMVWQSDNMS 426
             L+  RGS+CLYQGEELGL EAE+ F+ L DP GI FWP YKGRDGCRTPM W +D + 
Sbjct: 359 AFLLTQRGSICLYQGEELGLTEAELTFEQLVDPAGITFWPSYKGRDGCRTPMPWVADALH 418

Query: 427 GGFSIHRPWLPVSTEHLGLAVAVQEEAPDALLHHYRRALAFRRAHPALVKGDISDVTVVG 486
           GGF+   PWLPV   HL LAV  QE  P +LL+ YR  L FRR  PALVKGDI    + G
Sbjct: 419 GGFTTATPWLPVPEAHLALAVDRQERDPGSLLNAYRDFLLFRRNQPALVKGDIHYHPLQG 478

Query: 487 DVISFLRKDPEETVFVAINMSDAP-------------GAVDLPPGNWMQIGAELNSGGTS 533
           DVI F R   ++ + VA+N ++ P             GA  L  G W     EL + G +
Sbjct: 479 DVICFERLHGDDRLLVALNFANDPCTLSAPLDARPLAGAPSLVNGRWTAERLELPAFGIA 538

Query: 534 PDGRVHLGPWQP 545
                   PWQP
Sbjct: 539 IARCPSDLPWQP 550


Lambda     K      H
   0.321    0.138    0.451 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1090
Number of extensions: 39
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 552
Length of database: 550
Length adjustment: 36
Effective length of query: 516
Effective length of database: 514
Effective search space:   265224
Effective search space used:   265224
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources