Alignments for a candidate for xylT in Halomonas desiderata SP1

GapMind for catabolism of small carbon sources

Alignments for a candidate for xylT in Halomonas desiderata SP1

Align Glucose/fructose transport protein (characterized, see rationale)
to candidate WP_086511015.1 BZY95_RS16640 MFS transporter

Query= uniprot:A0A0H3C6H3
         (478 letters)



>NCBI__GCF_002151265.1:WP_086511015.1
          Length = 468

 Score =  569 bits (1467), Expect = e-167
 Identities = 275/454 (60%), Positives = 353/454 (77%), Gaps = 1/454 (0%)

Query: 24  FVAAIVAVATIGGFMFGYDSGVINGTQEGLESAFNLSKLGTGLNVGAILIGCAIGAFAAG 83
           ++  I  +A IGGF+FG+DSGVINGT +GL++AF    +GTG NV ++L+GCA+GAF AG
Sbjct: 12  YIFMICCIAAIGGFLFGFDSGVINGTVDGLQAAFGSDSVGTGFNVASMLLGCAVGAFFAG 71

Query: 84  RLADVWGRRTVMIISALLFVISAIGTGAAESSIVFIIFRLIGGLGVGAASVLCPVYISEV 143
           RLAD +GRRT++I++A+LF++SA G+G A SS+ F+ +R++GG+ VGAASV+ P YISEV
Sbjct: 72  RLADRFGRRTLLIVAAILFLVSAWGSGVAGSSLEFVFYRVLGGMAVGAASVMTPAYISEV 131

Query: 144 TPANIRGRLSSVQQIMIITGLTGAFVANYALAHTAGSSTAEFWLGLPAWRWMFWMQIIPA 203
            PA  RGRL+++QQ+ II+GL  AF++NY LAH +GS+ AE WLG   WRWMFW++++PA
Sbjct: 132 APAAYRGRLATIQQVAIISGLFVAFLSNYVLAHVSGSAMAELWLGFATWRWMFWIELLPA 191

Query: 204 GVFFLCLLGIPESPRYLVAKGKDAQAEAILSRLFGAGQGAKKVEEIRASLSADHKPTFSD 263
            VF L LL IPESPRYL++ GK  +A  +L  +   G+ + K+ EI A+L+ DHKP   D
Sbjct: 192 TVFLLALLFIPESPRYLISSGKQGEARRVLGLVMPEGEVSAKMAEIDATLARDHKPRLRD 251

Query: 264 LLDPTTKKLRVILWAGLVLAVFQQLVGINIVFYYGSVLWQSVGFTEDDSLKINILSGTLS 323
           +LD TT K+  I+W G+ LAVFQQLVGIN+VFYYG+VLWQSVGF+E D+L IN++SG +S
Sbjct: 252 VLDRTTGKVHGIVWVGIGLAVFQQLVGINVVFYYGAVLWQSVGFSEGDALLINVISGAVS 311

Query: 324 ILACLLAIGLIDKIGRKPLLLIGSAGMAVTLGVLTWCFSTATTVNGALTLGDQIGLTALI 383
           I ACLLAI LIDKIGRKPLL  GS GMA+TL  + + FSTAT ++G L LGD +G+ AL+
Sbjct: 312 IGACLLAIALIDKIGRKPLLWAGSVGMALTLACMAFAFSTATLIDGRLRLGDDMGVLALL 371

Query: 384 AANLYVIFFNLSWGPVMWVMLGEMFPNQMRGSALAVCGFAQWIANFAISVSFP-ALAAAS 442
           AAN YV FFN+SWGPVMWVMLGEMFPNQMRGS LA+ G  QW+ANF I+++FP  LA   
Sbjct: 372 AANAYVFFFNVSWGPVMWVMLGEMFPNQMRGSGLAIAGLFQWLANFGITMTFPIMLATIG 431

Query: 443 LPMTYGFYALSAVVSFFLVQKLVHETRGKELEAM 476
           L   YGFYAL AVVS F V +LV ETRGKELE M
Sbjct: 432 LAGAYGFYALCAVVSVFFVLRLVKETRGKELEEM 465


Lambda     K      H
   0.325    0.138    0.417 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 758
Number of extensions: 33
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 478
Length of database: 468
Length adjustment: 33
Effective length of query: 445
Effective length of database: 435
Effective search space:   193575
Effective search space used:   193575
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources