GapMind for Amino acid biosynthesis

 

Alignments for a candidate for ilvE in Shewanella sp. ANA-3

Align aromatic-amino-acid transaminase (EC 2.6.1.57) (characterized)
to candidate 7024947 Shewana3_2121 aromatic amino acid aminotransferase (RefSeq)

Query= BRENDA::P04693
         (397 letters)



>FitnessBrowser__ANA3:7024947
          Length = 397

 Score =  356 bits (913), Expect = e-103
 Identities = 181/397 (45%), Positives = 252/397 (63%), Gaps = 1/397 (0%)

Query: 1   MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIPQLQAVAEAEARLNAQP 60
           +F +V     DPIL L + FK DPR DKVNL +G+Y +E G  P LQ+V +AEA L  Q 
Sbjct: 2   IFSQVVLAPADPILGLTDTFKADPRQDKVNLGVGIYKDEAGQTPVLQSVKKAEALLLEQE 61

Query: 61  HGASLYLPMEGLNCYRHAIAPLLFGADHPVLKQQRVATIQTLGGSGALKVGADFLKRYFP 120
              + YL +EG+  Y   +  LLFG    ++   R AT Q  GG+GAL++ A+FL R  P
Sbjct: 62  KTKN-YLGIEGVQTYNRVVQELLFGEGSTLVTSGRAATAQAPGGTGALRIAAEFLLRNTP 120

Query: 121 ESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRFNDLLATLKTLPARSIVLLHP 180
              VWVS+PTW NH  IF  AG  V  Y +Y+ + + + F+ ++  L    A  IVLLH 
Sbjct: 121 SRTVWVSNPTWANHQNIFETAGLTVKEYGYYNASAHDIDFDGMMTDLANAQAGDIVLLHG 180

Query: 181 CCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGFGAGMEEDAYAIRAIASAGLPAL 240
           CCHNPTG DLT  QW+ V  +   ++L+P  D AYQGFGAG+EEDA  +R +A+     L
Sbjct: 181 CCHNPTGIDLTLAQWELVANLCADKQLVPLFDFAYQGFGAGIEEDAAGLRLVAAKVPELL 240

Query: 241 VSNSFSKIFSLYGERVGGLSVMCEDAEAAGRVLGQLKATVRRNYSSPPNFGAQVVAAVLN 300
           V+NSFSK F LY ER+G ++V+ ++A+ A R   Q+K T+R NYS+PP  GA +V+ +L+
Sbjct: 241 VANSFSKNFGLYNERIGAVTVVAQNADEAVRAFSQVKRTIRANYSNPPAHGALIVSTILS 300

Query: 301 DEALKASWLAEVEEMRTRILAMRQELVKVLSTEMPERNFDYLLNQRGMFSYTGLSAAQVD 360
           D ALKA W+ E+ EMR RI  MR   V+ L  E   ++F ++  Q GMFS++GL+  QV 
Sbjct: 301 DAALKALWVQELTEMRERIAQMRTLFVQSLKDEGVTQDFSFISRQNGMFSFSGLNKEQVA 360

Query: 361 RLREEFGVYLIASGRMCVAGLNTANVQRVAKAFAAVM 397
           RL++EFG+Y++ SGR+ VAG+   N+  + KA A V+
Sbjct: 361 RLKDEFGIYIVGSGRISVAGMTKTNMPVICKAIAQVL 397


Lambda     K      H
   0.320    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 412
Number of extensions: 13
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 397
Length adjustment: 31
Effective length of query: 366
Effective length of database: 366
Effective search space:   133956
Effective search space used:   133956
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory