Alignments for a candidate for hisD in Paraburkholderia bryophila 376MFSha3.1

GapMind for Amino acid biosynthesis

Alignments for a candidate for hisD in Paraburkholderia bryophila 376MFSha3.1

Align histidinol dehydrogenase (EC 1.1.1.23) (characterized)
to candidate H281DRAFT_05652 H281DRAFT_05652 histidinol dehydrogenase

Query= BRENDA::Q8G2R2
         (430 letters)



>FitnessBrowser__Burk376:H281DRAFT_05652
          Length = 440

 Score =  389 bits (1000), Expect = e-113
 Identities = 206/432 (47%), Positives = 285/432 (65%), Gaps = 5/432 (1%)

Query: 2   VTTLRQTDPDFEQKFAAFLSGKREVSEDVDRAVREIVDRVRREGDSALLDYSRRFDRIDL 61
           +  L  T  DF++   A L+ +    E ++R+V +I+  V+  GD+A+L+Y+ RFDR+  
Sbjct: 5   IRKLDSTARDFQKSLHAVLAFEASEDEAIERSVAQILSDVKARGDAAVLEYTNRFDRLSA 64

Query: 62  EKTG-IAVTEAEIDAAFDAAPASTVEALKLARDRIEKHHARQLPKDD----RYTDALGVE 116
                + +  +E++AA +        AL+ A  R+  +H +Q  +      +YT+A G  
Sbjct: 65  NSVAALELPMSELEAALEGLEPKRRAALEAAAARVRGYHEKQKIECGSHSWQYTEADGTV 124

Query: 117 LGSRWTAIEAVGLYVPGGTASYPSSVLMNAMPAKVAGVDRIVMVVPAPDGNLNPLVLVAA 176
           LG + T ++  G+YVPGG A+YPSSVLMNA+PA+VAGV  IVMVVP PDG  NPLVL AA
Sbjct: 125 LGQKVTPLDRAGIYVPGGKAAYPSSVLMNAIPARVAGVREIVMVVPTPDGVKNPLVLAAA 184

Query: 177 RLAGVSEIYRVGGAQAIAALAYGTETIRPVAKIVGPGNAYVAAAKRIVFGTVGIDMIAGP 236
            L GV  ++ +GGAQA+ ALAYGT+T+  V KI GPGNAYVA+AKR VFGTVGIDMIAGP
Sbjct: 185 LLGGVDRVFTIGGAQAVGALAYGTQTVPAVDKICGPGNAYVASAKRRVFGTVGIDMIAGP 244

Query: 237 SEVLIVADKDNNPDWIAADLLAQAEHDTAAQSILMTNDEAFAHAVEEAVERQLHTLARTE 296
           SE+L++ D   +P W+A DL +QAEHD  AQSIL+  D+AF   V +A+   L T+ R +
Sbjct: 245 SEILVLCDGTTDPRWVAMDLFSQAEHDELAQSILLCPDDAFIGRVNDAINELLPTMPRRD 304

Query: 297 TASASWRDFGAVILVKDFEDAIPLANRIAAEHLEIAVADAEAFVPRIRNAGSIFIGGYTP 356
              AS    GA+I V+D  +A  +AN IA EHLEI+  +   +   IR+AG+IF+G YT 
Sbjct: 305 VIQASLEGRGALIKVRDMAEACAIANDIAPEHLEISALEPHQWGQLIRHAGAIFLGRYTS 364

Query: 357 EVIGDYVGGCNHVLPTARSARFSSGLSVLDYMKRTSLLKLGSEQLRALGPAAIEIARAEG 416
           E +GDY  G NHVLPT+R+ARFSS L V D+ KR+S++++ +E  + LG  A E+A  EG
Sbjct: 365 ESLGDYCAGPNHVLPTSRTARFSSPLGVYDFFKRSSVIEVSAEGAQTLGEIAAELAYGEG 424

Query: 417 LDAHAQSVAIRL 428
           L AHA+S   R+
Sbjct: 425 LQAHARSAEYRM 436


Lambda     K      H
   0.318    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 449
Number of extensions: 25
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 440
Length adjustment: 32
Effective length of query: 398
Effective length of database: 408
Effective search space:   162384
Effective search space used:   162384
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

Align candidate H281DRAFT_05652 H281DRAFT_05652 (histidinol dehydrogenase)
to HMM TIGR00069 (hisD: histidinol dehydrogenase (EC 1.1.1.23))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00069.hmm
# target sequence database:        /tmp/gapView.3386160.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00069  [M=393]
Accession:   TIGR00069
Description: hisD: histidinol dehydrogenase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                -----------
   1.5e-159  517.4   0.0   1.7e-159  517.2   0.0    1.0  1  FitnessBrowser__Burk376:H281DRAFT_05652  


Domain annotation for each sequence (and alignments):
>> FitnessBrowser__Burk376:H281DRAFT_05652  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  517.2   0.0  1.7e-159  1.7e-159       1     393 []      37     435 ..      37     435 .. 0.98

  Alignments for each domain:
  == domain 1  score: 517.2 bits;  conditional E-value: 1.7e-159
                                TIGR00069   1 vkeiiedvrkeGdeAlleytekfdkv...kleslrvseeeleealeavdeelkealelaaeniekfhekq 67 
                                              v++i++dv+++Gd+A+leyt++fd++   ++++l+++ +ele+ale ++++ ++ale+aa++++ +hekq
  FitnessBrowser__Burk376:H281DRAFT_05652  37 VAQILSDVKARGDAAVLEYTNRFDRLsanSVAALELPMSELEAALEGLEPKRRAALEAAAARVRGYHEKQ 106
                                              789**********************96666678999********************************** PP

                                TIGR00069  68 lpes....veveteegvllgqkvrplervglYvPgGkaaypStvlmtavpAkvAgvkeivvvtPpkkdgk 133
                                              + e     +++++++g++lgqkv+pl+r+g+YvPgGkaaypS+vlm+a+pA+vAgv+eiv+v P+  dg 
  FitnessBrowser__Burk376:H281DRAFT_05652 107 KIECgshsWQYTEADGTVLGQKVTPLDRAGIYVPGGKAAYPSSVLMNAIPARVAGVREIVMVVPTP-DGV 175
                                              95433345*********************************************************6.*** PP

                                TIGR00069 134 vnpavlaaakllgvdevykvGGaqaiaalayGtetvpkvdkivGPGniyVtaAKklvfgevgidmiaGPs 203
                                               np vlaaa l gvd+v+++GGaqa++alayGt+tvp+vdki+GPGn+yV+ AK+ vfg+vgidmiaGPs
  FitnessBrowser__Burk376:H281DRAFT_05652 176 KNPLVLAAALLGGVDRVFTIGGAQAVGALAYGTQTVPAVDKICGPGNAYVASAKRRVFGTVGIDMIAGPS 245
                                              ********************************************************************** PP

                                TIGR00069 204 EvlviadesanpelvaaDllsqaEHdedaqailvttseelaekveeeveeqleelerkeiaeksleknga 273
                                              E+lv++d +++p++va+Dl+sqaEHde aq+il++++++++ +v+++++e l +++r++++++sle +ga
  FitnessBrowser__Burk376:H281DRAFT_05652 246 EILVLCDGTTDPRWVAMDLFSQAEHDELAQSILLCPDDAFIGRVNDAINELLPTMPRRDVIQASLEGRGA 315
                                              ********************************************************************** PP

                                TIGR00069 274 iilvddleealelsneyApEHLelqtkdpeellkkiknaGsvflGeytpealgdyvaGpnhvLPTsgtAr 343
                                              +i v+d++ea++++n++ApEHLe+ + +p+++ + i++aG++flG+yt+e+lgdy+aGpnhvLPTs+tAr
  FitnessBrowser__Burk376:H281DRAFT_05652 316 LIKVRDMAEACAIANDIAPEHLEISALEPHQWGQLIRHAGAIFLGRYTSESLGDYCAGPNHVLPTSRTAR 385
                                              ********************************************************************** PP

                                TIGR00069 344 fasglsvedFlkrisvqelskealeelaeaveklaeaEgLeaHaeavevR 393
                                              f+s+l+v+dF+kr+sv+e+s e++++l+e +++la  EgL+aHa+++e R
  FitnessBrowser__Burk376:H281DRAFT_05652 386 FSSPLGVYDFFKRSSVIEVSAEGAQTLGEIAAELAYGEGLQAHARSAEYR 435
                                              **********************************************9988 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (393 nodes)
Target sequences:                          1  (440 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 13.11
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis