Alignments for a candidate for prpE in Caulobacter crescentus NA1000

GapMind for Amino acid biosynthesis

Alignments for a candidate for prpE in Caulobacter crescentus NA1000

Align 4-hydroxybutyrate-CoA ligase (AMP-forming) (EC 6.2.1.40) (characterized)
to candidate CCNA_00192 CCNA_00192 long-chain-fatty-acid--CoA ligase

Query= BRENDA::A4YDR9
         (549 letters)



>FitnessBrowser__Caulo:CCNA_00192
          Length = 539

 Score =  224 bits (570), Expect = 9e-63
 Identities = 152/502 (30%), Positives = 246/502 (49%), Gaps = 14/502 (2%)

Query: 47  RYTYSTFYDNVMVQASALMRRGFSREDKLSFISRNRPEFLESFFGVPYAGGVLVPINFRL 106
           RY Y+         A AL R G    D+++ ++ N    LE F+  P  G VL   N RL
Sbjct: 40  RYDYAGLAGRAAQAAHALRRLGIKAGDRVTSLAWNTHRHLELFYAAPGIGAVLHTANPRL 99

Query: 107 SPKEMAYIINHSDSKFVVVDEPYLNSLLEVKDQIKA--EIILLEDPDNPSASETARKEVR 164
             +++ Y INH++S  +  +  +   +  +  Q+      ++L D +             
Sbjct: 100 FDEQIVYTINHAESGVLFFERNFQALVERIAPQLTTVKTFVMLSDAERTVPGAVGA---- 155

Query: 165 MTYRELVKGGSRDPLPIPAKEEYSMITLYYTSGTTGLPKGVMHHHRGAFLNAMAEVLEHQ 224
           ++Y  L++G   D +  P+ +E +   L YTSGTTG PKGV++ HR   L+AMA  L   
Sbjct: 156 ISYETLIEG-EPDVIAWPSFDENAGAFLCYTSGTTGDPKGVLYSHRAVVLHAMAGGLNSA 214

Query: 225 MDLN--SVYLWTLPMFHAASWGFSWATVAVGATNVC-LDKVDYPLIYRLVEKERVTHMCA 281
                  V +    ++HA +WG  ++    GA  V   DK+D   +++L+E E VT    
Sbjct: 215 FGFTPFDVVMPCSSLYHATAWGLPFSAPICGAKLVLPADKMDGASLHQLIEGEGVTFTGG 274

Query: 282 APTVYVNLADYMKRNNLKFSNRVHMLVAGAAPAPATLKAMQE-IGGYMCHVYGLTETYGP 340
            PT++    D++ +N+ +  +   +++ G+A   A  +  +   G     ++G+TET   
Sbjct: 275 VPTIWTMYLDWLDKNDRRPDSLKKVVIGGSAVPRAMAETFKRRYGVQTLQIWGMTETCPI 334

Query: 341 HSICEWRREWDSLPLEEQAK-LKARQGIPYVSFEMDVFDANGKPVPWDGKTIGEVVMRGH 399
             +        +L  E   + +  RQG      E+ V   +G+  PWDG+T G +++RG 
Sbjct: 335 GVVATPTPALAALGEEAMDEAIWTRQGRLQFGIELKVETEDGQAAPWDGETSGALLVRGP 394

Query: 400 NVALGYYKNPEKTAESFRDGWFHSGDAAVVHPDGYIEIVDRFKDLINTGGEKVSSILVEK 459
            V   Y++     A    DG+F +GD A +  +G++ I DR KD+I +GGE +SSI +E 
Sbjct: 395 WVVKRYFRKDADAARE--DGFFDTGDIATLDANGFMRITDRQKDVIKSGGEWISSIDLEN 452

Query: 460 TLMEIPGVKAVAVYGTPDEKWGEVVTARIELQEGVKLTEEEVIKFCKERLAHFECPKIVE 519
             +  PGVK  AV G P  KW E     IE  EG ++T+  V+ +   R+  +  P  V 
Sbjct: 453 VAVGCPGVKIAAVIGVPHPKWEERPLLVIETHEGAEVTKAAVLDYLTPRIVKWWTPDDVV 512

Query: 520 FGPIPMTATGKMQKYVLRNEAK 541
           F  +P+TATGK+ K VLR   K
Sbjct: 513 FATVPLTATGKIDKKVLRQAWK 534


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 700
Number of extensions: 34
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 549
Length of database: 539
Length adjustment: 35
Effective length of query: 514
Effective length of database: 504
Effective search space:   259056
Effective search space used:   259056
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis