Alignments for a candidate for aroG in Echinicola vietnamensis KMM 6221, DSM 17526

GapMind for Amino acid biosynthesis

Alignments for a candidate for aroG in Echinicola vietnamensis KMM 6221, DSM 17526

Align 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54) (characterized)
to candidate Echvi_2512 Echvi_2512 phospho-2-dehydro-3-deoxyheptonate aldolase

Query= BRENDA::Q9WYH8
         (338 letters)



>FitnessBrowser__Cola:Echvi_2512
          Length = 339

 Score =  280 bits (716), Expect = 4e-80
 Identities = 145/329 (44%), Positives = 209/329 (63%), Gaps = 2/329 (0%)

Query: 1   MIVVLKPGSTEEDIRKVVKLAESYNLKCHISKGQERTVIGIIGDDRYVVADKFESLDCVE 60
           MI+ +K   TE    +++K       K      Q+ T +  IG   + +  KF   + ++
Sbjct: 1   MIIQVKQDITEVQKERLIKEINQIGYKITEVITQKGTYLVGIGSAEFDIR-KFGHHEGIQ 59

Query: 61  SVVRVLKPYKLVSREFHPEDTVIDLGD-VKIGNGYFTIIAGPCSVEGREMLMETAHFLSE 119
            +  V   YKLVSR++    T IDLGD V I  G   ++AGPCS+E  E +++    L  
Sbjct: 60  DIHIVSDAYKLVSRKWKVNPTSIDLGDGVYIKEGDMAVMAGPCSIESEEQIVKVIDHLKA 119

Query: 120 LGVKVLRGGAYKPRTSPYSFQGLGEKGLEYLREAADKYGMYVVTEALGEDDLPKVAEYAD 179
             +K++RGG YKPR+SPY+F+GLG +GL+   E A K G+ ++TE +    + ++ +Y D
Sbjct: 120 NNIKIMRGGVYKPRSSPYAFRGLGIEGLKLWHELASKAGIKIITEVMQVSQIEEMMDYVD 179

Query: 180 IIQIGARNAQNFRLLSKAGSYNKPVLLKRGFMNTIEEFLLSAEYIANSGNTKIILCERGI 239
           + Q+GARN QNF LL + G  +KPV++KRG   TIEE L SAEY+ + GN K+ILCERGI
Sbjct: 180 VFQVGARNTQNFNLLDELGKVDKPVMIKRGISGTIEELLQSAEYVFSGGNEKLILCERGI 239

Query: 240 RTFEKATRNTLDISAVPIIRKESHLPILVDPSHSGGRRDLVIPLSRAAIAVGAHGIIVEV 299
           RT+EKATRNTLD++AVP+++ +SHLP++VDPSH  G R  V  ++ A +  GA GII E 
Sbjct: 240 RTYEKATRNTLDLNAVPVLKDKSHLPVVVDPSHGIGIRKFVHQMALAGVMAGADGIIYET 299

Query: 300 HPEPEKALSDGKQSLDFELFKELVQEMKK 328
           H  PEKA SDG+Q+LDF    +L  +++K
Sbjct: 300 HEIPEKAYSDGQQTLDFAQSSQLASQIRK 328


Lambda     K      H
   0.318    0.138    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 326
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 338
Length of database: 339
Length adjustment: 28
Effective length of query: 310
Effective length of database: 311
Effective search space:    96410
Effective search space used:    96410
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate Echvi_2512 Echvi_2512 (phospho-2-dehydro-3-deoxyheptonate aldolase)
to HMM TIGR01361 (aroF: 3-deoxy-7-phosphoheptulonate synthase (EC 2.5.1.54))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01361.hmm
# target sequence database:        /tmp/gapView.93151.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01361  [M=260]
Accession:   TIGR01361
Description: DAHP_synth_Bsub: 3-deoxy-7-phosphoheptulonate synthase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                        -----------
     8e-106  339.1   0.1     1e-105  338.8   0.1    1.1  1  FitnessBrowser__Cola:Echvi_2512  


Domain annotation for each sequence (and alignments):
>> FitnessBrowser__Cola:Echvi_2512  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  338.8   0.1    1e-105    1e-105       2     258 ..      70     327 ..      69     329 .. 0.98

  Alignments for each domain:
  == domain 1  score: 338.8 bits;  conditional E-value: 1e-105
                        TIGR01361   2 laskkvkkeetvvdved.vkiGegeliviaGPCsveseeqivetakavkeaGakllrGgafkPrtsPysfqGlgeegl 78 
                                      l+s+k+k + t +d+ d v i eg+++v+aGPCs+eseeqiv++  ++k++ +k++rGg++kPr sPy+f+Glg egl
  FitnessBrowser__Cola:Echvi_2512  70 LVSRKWKVNPTSIDLGDgVYIKEGDMAVMAGPCSIESEEQIVKVIDHLKANNIKIMRGGVYKPRSSPYAFRGLGIEGL 147
                                      7899*****999999755************************************************************ PP

                        TIGR01361  79 kllkrakdetgllvvtevlderdveivaeyvDilqiGarnmqnfelLkevgkskkPvlLkrglaatieewleaaeYil 156
                                      kl ++ ++++g++++tev++++++e + +yvD++q+Garn+qnf+lL+e+gk +kPv++krg++ tiee+l++aeY+ 
  FitnessBrowser__Cola:Echvi_2512 148 KLWHELASKAGIKIITEVMQVSQIEEMMDYVDVFQVGARNTQNFNLLDELGKVDKPVMIKRGISGTIEELLQSAEYVF 225
                                      ****************************************************************************** PP

                        TIGR01361 157 segnenvilcerGirtfekatrftldlsavallkklthlPvivDpshaaGrrdlvlplakaavavGadgllievhpdP 234
                                      s gne++ilcerGirt+ekatr+tldl+av++lk+++hlPv+vDpsh+ G r++v+ +a a+v +Gadg++ e h+ P
  FitnessBrowser__Cola:Echvi_2512 226 SGGNEKLILCERGIRTYEKATRNTLDLNAVPVLKDKSHLPVVVDPSHGIGIRKFVHQMALAGVMAGADGIIYETHEIP 303
                                      ****************************************************************************** PP

                        TIGR01361 235 ekalsDseqqltpeefkelvkelk 258
                                      eka sD++q l++++ ++l ++++
  FitnessBrowser__Cola:Echvi_2512 304 EKAYSDGQQTLDFAQSSQLASQIR 327
                                      *************99999888776 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (260 nodes)
Target sequences:                          1  (339 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 28.81
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis