Alignments for a candidate for cimA in Phaeobacter inhibens BS107

Align (R)-citramalate synthase (EC 2.3.3.21) (characterized)
to candidate GFF1624 PGA1_c16460 2-isopropylmalate synthase/homocitrate synthase-like protein

Query= BRENDA::Q74C76
         (528 letters)



>FitnessBrowser__Phaeo:GFF1624
          Length = 543

 Score =  424 bits (1090), Expect = e-123
 Identities = 243/542 (44%), Positives = 336/542 (61%), Gaps = 31/542 (5%)

Query: 6   LYDTTLRDGTQAEDISFLVEDKIRIAHKLDEIGIHYIEGGWPGSNPKDVAFFKDIKKEKL 65
           LYDTTLRDG Q + + F   +K++IA  LD +G+ YIEGGWPG+NP D  FF    +   
Sbjct: 8   LYDTTLRDGQQTQGVQFSTTEKVQIATALDGLGVDYIEGGWPGANPTDSGFFDAAPR--- 64

Query: 66  SQAKIAAFGSTRRAKVTPDKDHNLKTLIQAEPDVCTIFGKTWDFHVHEALRISLEENLEL 125
           ++A + AFG T+RA  + + D  L  ++ A      + GK+ D+HV  AL I+LEENL+ 
Sbjct: 65  TRATMTAFGMTKRAGRSAENDDVLAAVLNAGTAAVCLVGKSHDYHVTHALGITLEENLDN 124

Query: 126 IFDSLEYLKANVPEVFYDAEHFFDGYKANPDYAIKTLKAAQDAKADCIVLCDTNGGTMPF 185
           I  S+ +L A   E  +DAEHFFDGYK NPDYA+   +AA +A A  +VLCDTNGGT+P 
Sbjct: 125 IRASIAHLVAQGREALFDAEHFFDGYKDNPDYALAACRAALEAGARWVVLCDTNGGTLPG 184

Query: 186 ELVEIIREVRKHITAPL-----GIHTHNDSECAVANSLHAVSEGIVQVQGTINGFGERCG 240
           ++  I+ EV   I A L     GIHTHND+E AVA SL AV  G  Q+QGT+NG GERCG
Sbjct: 185 DVGRIVAEV---IAAGLPGDHLGIHTHNDTENAVACSLAAVDAGARQIQGTLNGLGERCG 241

Query: 241 NANLCSIIPALKLKMKREC-----IGDDQLRKLRDLSRFVYELANLSPNKHQAYVGNSAF 295
           NANL ++IP L LK          +  + L  L  LSR + E+ N  P K  AYVG SAF
Sbjct: 242 NANLTTLIPTLLLKPPYADQFDIGVSHEGLSTLTALSRMLDEILNRVPTKQAAYVGASAF 301

Query: 296 AHKGGVHVSAIQRHPETYEHLRPELVGNMTRVLVSDLSGRSNILAKAEEFNIKMDSKDPV 355
           AHK G+H SAI + P TYEH+ P LVGN   + +S+ +G+SN+  +  E  +++++ DP 
Sbjct: 302 AHKAGLHASAILKDPSTYEHIDPALVGNARIIPMSNQAGQSNLRRRLSEAGLRVENGDPA 361

Query: 356 TLEILENIKEMENRGYQFEGAEASFELLMKRALGTHRKFFSVIGFRVIDEKR---HEDQK 412
              ILE IK  E  GY ++ A+ASFE+L +  LG    FF V  ++V  E+R   ++   
Sbjct: 362 LARILERIKTREAEGYSYDTAQASFEILAREELGQLPSFFEVKRYKVTVERRKNKYDRMV 421

Query: 413 PLSEATIMVKVGGK--------IEHTAAEGNGPVNALDNALRKALEKFYPRLKEVKLLDY 464
            LSEA ++VKV G+        ++ T ++  GPVNAL  AL K L ++   L +++L+D+
Sbjct: 422 SLSEAVVVVKVDGQKLLSVSESLDETGSD-RGPVNALAKALTKDLGQYSKALDDMRLVDF 480

Query: 465 KVRVLPAGQGTASSIRVLIESGDKES-RWGTVGVSENIVDASYQALLDSVEYKLHKSEEI 523
           KVR+     GT +  RV+I+S D    RW TVGVS NI+DAS++ALLD++ +KL +  + 
Sbjct: 481 KVRITQG--GTEAVTRVIIDSEDGAGRRWSTVGVSANIIDASFEALLDAIRWKLLRDTDA 538

Query: 524 EG 525
            G
Sbjct: 539 GG 540


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 692
Number of extensions: 30
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 528
Length of database: 543
Length adjustment: 35
Effective length of query: 493
Effective length of database: 508
Effective search space:   250444
Effective search space used:   250444
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate GFF1624 PGA1_c16460 (2-isopropylmalate synthase/homocitrate synthase-like protein)
to HMM TIGR00977 (cimA: citramalate synthase (EC 2.3.1.182))

Software error:

../bin/blast/fastacmd -i /tmp/list.30142.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.30142.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

For help, please send mail to the webmaster (help@microbesonline.org), giving this error message and the time and date of the error.