Alignments for a candidate for glnA in Pseudomonas putida KT2440

GapMind for Amino acid biosynthesis

Alignments for a candidate for glnA in Pseudomonas putida KT2440

Align glutamine synthetase (EC 6.3.1.2) (characterized)
to candidate PP_5184 PP_5184 glutamylpolyamine synthetase

Query= BRENDA::P36205
         (439 letters)



>FitnessBrowser__Putida:PP_5184
          Length = 458

 Score =  197 bits (502), Expect = 4e-55
 Identities = 137/449 (30%), Positives = 223/449 (49%), Gaps = 32/449 (7%)

Query: 11  EEENVRFIRLQFTDINGTLKNLEITPDVFLESWEDGIMF---------DGSSIEGF---V 58
           E   V ++ L   D+NG ++   I      + +E GI           +GS++E     +
Sbjct: 19  EHPEVLYVDLLIADMNGVVRGKRIERTSLHKVYEKGINLPASLFALDINGSTVESTGLGL 78

Query: 59  RIEESDMYLKPVLDTFAVLPWTVDGAKSARVICDVYTPDGKPFEGDPRYRLRRMMEKAEQ 118
            I ++D    P+ DT    PW      +A+++  ++  +G+PF  DPR  LR+++ K ++
Sbjct: 79  DIGDADRICYPIPDTLCNEPW--QKRPTAQLLMTMHELEGEPFFADPREVLRQVVTKFDE 136

Query: 119 LGYTPYAGPEMEFFILPI-NEKGEPVPEFLDHGG-------YFDLLPLSKVEEIRRDIAI 170
           LG T  A  E+EF+++   N  G P P      G        + +  L +  +  +DI  
Sbjct: 137 LGLTICAAFELEFYLIDQENVNGRPQPPRSPISGKRPQSTQVYLIDDLDEYADCLQDILE 196

Query: 171 ALEKMGITVEATHHEVAPSQHEVDFRYDTF-LRTADNAQTVKLVIKTMAIFHGYHATFMP 229
             ++ GI  +A   E AP+Q EV+  +    L+  D A  +K +IK +A  H    TFM 
Sbjct: 197 GAKEQGIPADAIVKESAPAQFEVNLHHVADPLKACDYAVLLKRLIKNIAYDHEMDTTFMA 256

Query: 230 KPFYGVNGSGMHVHMSLFRGDKNAFYDPDDPLGLSKELRYFVGGILKHAKALAAVTNPTI 289
           KP+ G  G+G+HVH+S+   D N  +  +DP   +  LR+ VGG+L+   A  A   P +
Sbjct: 257 KPYPGQAGNGLHVHISVLDKDGNNIFTSEDP-EQNAALRHAVGGVLETLPASMAFLCPNV 315

Query: 290 NSYKRLVPGYEAPVYISWSVGNRSALIRIPKARGKATRLEYRSPDPSCNIYLAFAAILAA 349
           NSY+R    +  P   SW + NR+  +R+P     A R+E+R      N YL  AA+LA 
Sbjct: 316 NSYRRFGAQFYVPNAPSWGLDNRTVALRVPTGSADAVRIEHRVAGADANPYLMMAAVLAG 375

Query: 350 GLDGIINKIEPPAPVEENIYHMTSERREELNIESLPGSLKEAVEELKKDDVIIDALGEHI 409
              G+ NKIEP AP+E N Y       E+L  +SLP +L++A+ EL   +++   +    
Sbjct: 376 VHHGLTNKIEPGAPIEGNSY-------EQLE-QSLPNNLRDALRELDDSEILNKYIDPKY 427

Query: 410 FEKFVEAAEKDWKEFSTYVTNWELQRYLY 438
            + FV   E + +EF   +++ E   YL+
Sbjct: 428 IDIFVACKESELEEFEHSISDLEYNWYLH 456


Lambda     K      H
   0.320    0.139    0.414 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 503
Number of extensions: 19
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 439
Length of database: 458
Length adjustment: 33
Effective length of query: 406
Effective length of database: 425
Effective search space:   172550
Effective search space used:   172550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis