prs, hisG, hisI, hisE, hisA, hisF, hisH, hisB, hisC, hisN, hisD
Also see fitness data for the top candidates
Overview: Histidine biosynthesis in GapMind is based on the MetaCyc pathway (link).
Or see definitions of steps
| Step | Description | Best candidate | 2nd candidate |
|---|---|---|---|
| prs | ribose-phosphate diphosphokinase | Ac3H11_3452 | Ac3H11_2203 |
| hisG | ATP phosphoribosyltransferase | Ac3H11_4722 | |
| hisI | phosphoribosyl-ATP pyrophosphatase | Ac3H11_4736 | Ac3H11_4724 |
| hisE | phosphoribosyl-AMP cyclohydrolase | Ac3H11_4735 | Ac3H11_4724 |
| hisA | 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase | Ac3H11_4732 | Ac3H11_4733 |
| hisF | imidazole glycerol phosphate synthase, cyclase subunit | Ac3H11_4733 | Ac3H11_4732 |
| hisH | imidazole glycerol phosphate synthase, amidotransferase subunit | Ac3H11_4731 | |
| hisB | imidazoleglycerol-phosphate dehydratase | Ac3H11_4730 | |
| hisC | histidinol-phosphate aminotransferase | Ac3H11_4729 | Ac3H11_3720 |
| hisN | histidinol-phosphate phosphatase | Ac3H11_1314 | Ac3H11_4730 |
| hisD | histidinal/histidinol dehydrogenase | Ac3H11_4724 |
Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory