Alignments for a candidate for metC in Pseudomonas fluorescens FW300-N2E2

GapMind for Amino acid biosynthesis

Alignments for a candidate for metC in Pseudomonas fluorescens FW300-N2E2

Align cystathionine gamma-lyase (EC 4.4.1.1) (characterized)
to candidate Pf6N2E2_4958 Cystathionine gamma-synthase (EC 2.5.1.48)

Query= BRENDA::Q5H4T8
         (397 letters)



>FitnessBrowser__pseudo6_N2E2:Pf6N2E2_4958
          Length = 417

 Score =  266 bits (681), Expect = 6e-76
 Identities = 160/383 (41%), Positives = 220/383 (57%), Gaps = 12/383 (3%)

Query: 19  TLAIHGGQSPDPSTGAVMPPIYATSTYAQS--------SPGEHQGFEYSRTHNPTRFAYE 70
           T A+ GG+       A   PI A++ Y           + G+  GF YSR  NPT    E
Sbjct: 17  TRAVWGGEQVRHPYNATQTPIVASAAYGYDDIDVWYDVALGKAPGFIYSRMSNPTVETLE 76

Query: 71  RCVAALEGGTRAFAFASGMAATSTVM-ELLDAGSHVVAMDDLYGGTFRLFERVRRRTAGL 129
             +  LE    A AF+SGMAA S+V+   L  G  VV+  D YGGT ++FE    RT G+
Sbjct: 77  AKIRELEMAESAVAFSSGMAAISSVLYTFLAHGDRVVSTKDSYGGTNKIFEEFLPRT-GV 135

Query: 130 DFSFVDLTDPAAFKAAIRADTKMVWIETPTNPMLKLVDIAAIAVIARKHGLLTVVDNTFA 189
             +  +  D    +  I    +++++ETPTNP LK++DI  +   A++ G + V DNTFA
Sbjct: 136 AVTLCETFDHDDLEREIAKGCQVLYLETPTNPTLKILDIPRLVAAAKRVGAVVVADNTFA 195

Query: 190 SPMLQRPLSLGADLVVHSATKYLNGHSDMVGGIAVVGDNAELAEQMAFLQNSIGGVQGPF 249
           +P+ Q PL+LG D+V+HSATK+L+GH D++GG+ V G  A +A Q+   +   G    PF
Sbjct: 196 TPLNQSPLALGVDVVIHSATKFLSGHGDVLGGL-VCGSEALMA-QVRHYREINGASLDPF 253

Query: 250 DSFLALRGLKTLPLRMRAHCENALALAQWLETHPAIEKVIYPGLASHPQHVLAKRQMSGF 309
            ++L +RG+KTL LRMR    +A ALA++L T P +E V YPGL SHP H +A  QMSGF
Sbjct: 254 SAYLIIRGMKTLALRMRQQQHSARALAEFLCTEPLVEAVNYPGLPSHPNHAVACAQMSGF 313

Query: 310 GGIVSIVLKGGFDAAKRFCEKTELFTLAESLGGVESLVNHPAVMTHASIPVARREQLGIS 369
           G IVS VL GG D  K    +      A +LG VE++       +H    +  R  LGIS
Sbjct: 314 GAIVSFVLVGGMDTVKLLLPRLRFAHCAGNLGAVETIYGPARTTSHVENTLEERLALGIS 373

Query: 370 DALVRLSVGIEDLGDLRGDLERA 392
           + LVR+SVGIED  DL  DL++A
Sbjct: 374 EGLVRVSVGIEDTDDLLDDLKQA 396


Lambda     K      H
   0.320    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 469
Number of extensions: 20
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 397
Length of database: 417
Length adjustment: 31
Effective length of query: 366
Effective length of database: 386
Effective search space:   141276
Effective search space used:   141276
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis