Alignments for a candidate for argC in Trichodesmium erythraeum IMS101 Annotated Metagenome-Assembled Genome

GapMind for Amino acid biosynthesis

Alignments for a candidate for argC in Trichodesmium erythraeum IMS101 Annotated Metagenome-Assembled Genome

Align N-acetyl-gamma-glutamyl-phosphate reductase; Short=AGPR; EC 1.2.1.38 (characterized, see rationale)
to candidate 2629597979 Ga0074568_1143 N-acetyl-gamma-glutamyl-phosphate reductase

Query= uniprot:E4PLW0
         (388 letters)



>IMG__TrieryIMS101_FD:2629597979
          Length = 352

 Score =  289 bits (739), Expect = 1e-82
 Identities = 147/346 (42%), Positives = 213/346 (61%), Gaps = 2/346 (0%)

Query: 45  IKVGIVGGTGYTGVELLRILAVHPEVSVSCITSRSEAGMPVAEMYPNLRGHYDLAFSEPD 104
           + +GIVG +GY GV+L+++L  HP+V V  +   S AG P + +YP+L    DL     +
Sbjct: 7   VPIGIVGASGYGGVQLVKLLQNHPKVEVVYLGGDSSAGKPFSSIYPHLEHSVDLKIESIE 66

Query: 105 VN-VLGACDLVFFATPHGVAMRMVPELMSAGVRVVDLSADFRLKDLDVWANWYGMAHESP 163
           ++ +     +VF + P+G+A +M P L+  G +V+DLSAD+R  +L+ + +WYG      
Sbjct: 67  LDKIANKSQIVFLSLPNGLAWKMAPNLLEKGCKVLDLSADYRFSNLETYQSWYGGERTDQ 126

Query: 164 EWAEKAVYGLPEVVRDEIRNAQLVANPGCYPTAVQLGFLPLLEQGLVDPKRLIADAKSGA 223
           + A  AVYGLPE+ RD I NAQL+  PGCY TA  L   PL +QGL+ P+  I DAKSG 
Sbjct: 127 KIAATAVYGLPELYRDRIANAQLIGCPGCYVTASLLALSPLFKQGLIVPETAIIDAKSGT 186

Query: 224 SGAGRQGKIGMLHGEIGESFKAYGASGHRHLPEIRQGLCGAAGGDVGVTFVPHLIPMIRG 283
           SG GR+  I ML  E   S   YG + HRH PEI Q     AG DV V F PHL+PM+RG
Sbjct: 187 SGGGRKANINMLLAEADNSVGPYGVASHRHTPEIEQICSNLAGYDVKVQFTPHLMPMVRG 246

Query: 284 IEATLYAELKNPADF-DRLQALFEQRFDDEPFVDVMPFGSHPETRSVRGANQCRMALHRQ 342
           I AT+Y  L++P    + L  ++   + + P+V V+P G++P+T+   G N C + +   
Sbjct: 247 ILATVYGTLRDPGLVREDLLTIYNAFYRNCPWVKVLPSGTYPQTKWASGTNLCYLGIEVD 306

Query: 343 EQSNIVIVSSVIDNLVKGAAGQAVQNMNIMFGLKETMGLEAPALLP 388
            +++ VI+ S IDNL+KG AGQ++Q MN+M G +ET+GL   +  P
Sbjct: 307 HRTDRVIIMSAIDNLIKGQAGQSIQCMNLMMGWEETLGLPQLSFYP 352


Lambda     K      H
   0.321    0.137    0.412 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 380
Number of extensions: 16
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 388
Length of database: 352
Length adjustment: 30
Effective length of query: 358
Effective length of database: 322
Effective search space:   115276
Effective search space used:   115276
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 49 (23.5 bits)

Align candidate 2629597979 Ga0074568_1143 (N-acetyl-gamma-glutamyl-phosphate reductase)
to HMM TIGR01850 (argC: N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR01850.hmm
# target sequence database:        /tmp/gapView.1883943.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01850  [M=345]
Accession:   TIGR01850
Description: argC: N-acetyl-gamma-glutamyl-phosphate reductase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                        Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                        -----------
   1.3e-136  441.3   0.0   1.5e-136  441.1   0.0    1.0  1  IMG__TrieryIMS101_FD:2629597979  


Domain annotation for each sequence (and alignments):
>> IMG__TrieryIMS101_FD:2629597979  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  441.1   0.0  1.5e-136  1.5e-136       3     345 .]       9     352 .]       7     352 .] 0.99

  Alignments for each domain:
  == domain 1  score: 441.1 bits;  conditional E-value: 1.5e-136
                        TIGR01850   3 vaivGasGYtGaeLlrllakHpevevtklvssreagkklsevhphlkglvdlkleeleeeeileeadvvflAlphgvs 80 
                                      ++ivGasGY+G++L++ll++Hp+vev++l ++++agk++s+++phl++ vdlk+e++e ++i+++ ++vfl+lp+g +
  IMG__TrieryIMS101_FD:2629597979   9 IGIVGASGYGGVQLVKLLQNHPKVEVVYLGGDSSAGKPFSSIYPHLEHSVDLKIESIELDKIANKSQIVFLSLPNGLA 86 
                                      89**************************************************************************** PP

                        TIGR01850  81 aelvpellekgvkvidlSadfRlkdaevYekwYgkkhekeelleeavYGlpElnreeikkaklianPGCyaTaalLal 158
                                       +++p+llekg+kv+dlSad+R+++ e+Y++wYg ++++++++++avYGlpEl+r++i++a+li++PGCy Ta+lLal
  IMG__TrieryIMS101_FD:2629597979  87 WKMAPNLLEKGCKVLDLSADYRFSNLETYQSWYGGERTDQKIAATAVYGLPELYRDRIANAQLIGCPGCYVTASLLAL 164
                                      ****************************************************************************** PP

                        TIGR01850 159 aPllkekliepksiivdaksGvSgAGrkasekslfaevnenlkpYkvtkHrHtpEieqelsklaekkvkvsftphlvp 236
                                      +Pl+k++li p+++i+daksG+Sg+Grka+ ++l+ae+++++ pY v++HrHtpEieq +s+la+ +vkv+ftphl+p
  IMG__TrieryIMS101_FD:2629597979 165 SPLFKQGLIVPETAIIDAKSGTSGGGRKANINMLLAEADNSVGPYGVASHRHTPEIEQICSNLAGYDVKVQFTPHLMP 242
                                      ****************************************************************************** PP

                        TIGR01850 237 mtrGilatiyaklkke.lteeelrklyeevYedepfvrvlkegelPstkavlgsnfvdigvavdeetkrvvvvsaiDN 313
                                      m+rGilat+y +l+++ l +e+l ++y+++Y++ p+v+vl++g++P+tk+  g+n + +g++vd++t+rv+++saiDN
  IMG__TrieryIMS101_FD:2629597979 243 MVRGILATVYGTLRDPgLVREDLLTIYNAFYRNCPWVKVLPSGTYPQTKWASGTNLCYLGIEVDHRTDRVIIMSAIDN 320
                                      *************9987************************************************************* PP

                        TIGR01850 314 LvKGaagqAvqnlNlmlgfdetegLeklpllp 345
                                      L+KG+agq +q++Nlm+g++et gL++l+++p
  IMG__TrieryIMS101_FD:2629597979 321 LIKGQAGQSIQCMNLMMGWEETLGLPQLSFYP 352
                                      ***************************99998 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (345 nodes)
Target sequences:                          1  (352 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00
# Mc/sec: 24.34
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis