GapMind for Amino acid biosynthesis

 

Protein WP_048065228.1 in Methanosarcina acetivorans C2A

Annotation: NCBI__GCF_000007345.1:WP_048065228.1

Length: 383 amino acids

Source: GCF_000007345.1 in NCBI

Candidate for 6 steps in Amino acid biosynthesis

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-cysteine biosynthesis serC hi phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) (characterized) 45% 99% 320.1 Serine-pyruvate aminotransferase; SAT; EC 2.6.1.51 39% 288.9
L-lysine biosynthesis dapC hi phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) (characterized) 45% 99% 320.1 Serine-pyruvate aminotransferase; SAT; EC 2.6.1.51 39% 288.9
L-phenylalanine biosynthesis PPYAT hi phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) (characterized) 45% 99% 320.1 Serine-pyruvate aminotransferase; SAT; EC 2.6.1.51 39% 288.9
L-serine biosynthesis serC hi phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) (characterized) 45% 99% 320.1 Serine-pyruvate aminotransferase; SAT; EC 2.6.1.51 39% 288.9
glycine biosynthesis agx1 lo asparagine-oxo-acid transaminase (EC 2.6.1.14); alanine-glyoxylate transaminase (EC 2.6.1.44); serine-glyoxylate transaminase (EC 2.6.1.45) (characterized) 34% 94% 206.1 phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) 45% 320.1
L-serine biosynthesis serB lo Phosphoserine aminotransferase; EC 2.6.1.52 (characterized, see rationale) 35% 94% 176 phosphoserine aminotransferase monomer (EC 2.6.1.1; EC 2.6.1.52) 45% 320.1

Sequence Analysis Tools

View WP_048065228.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MDLEDTLLMMPGPVPVTPRVLRAMSKPMINHRSAEFAGIYTDCRQILADVFQTKNDIFLL
SGSGTAGMEAAVGSVAGSGDKVIAIENGKFGQRFKDLAALYADVVPLEFEWGLPVDLEMV
KEKLEEGAKAITLVHNETSAGIMNPAVEIGKLAKKHDAIFIMDGVTSLGGDEVKVDEWGV
DIAIVGSQKCLAAPPGMSAVSVSEKAFEAINSMKKRPYYNDLKAYKKSGDKPRPETPYTP
ALPLFYAMQEALHIVKEEGMEVRIKRHRALSEAVRAAAGAMNIEMFPQLNEYSKYSNTVT
AMKAPAGVDGEDVKNDMKKRGVIIAGGQEHLKGKIFRIGNMGNVTARDVLSTIQQLEIVL
SKQGYIDSVGAGAEAAMRVIDRV

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory