GapMind for Amino acid biosynthesis


Potential Gaps in Amino acid biosynthesis in Methanothermobacter thermautotrophicus Delta H

Found 4 low-confidence and 8 medium-confidence steps on the best paths for 18 pathways. 3 of 12 gaps have been classified.

Pathway Step Best candidate 2nd candidate Class of gap
arg argC: N-acylglutamylphosphate reductase MTH_RS03985  
arg carB: carbamoyl phosphate synthase subunit beta spurious
asn asnB: asparagine synthase (glutamine-hydrolysing) MTH_RS01915  
chorismate aroE: shikimate dehydrogenase MTH_RS04785 spurious
chorismate fbp: 6-deoxy-5-ketofructose 1-phosphate synthase MTH_RS08075 MTH_RS02685  
his hisN: histidinol-phosphate phosphatase MTH_RS07010  
lys dapA: 4-hydroxy-tetrahydrodipicolinate synthase spurious
met asd-S-ferredoxin: reductive sulfuration of L-aspartate semialdehyde, ferredoxin component MTH_RS04025 MTH_RS05920  
met asd-S-perS: putative persulfide forming protein MTH_RS03410  
pro argC: N-acylglutamylphosphate reductase MTH_RS03985  
thr homK: putative homoserine phosphotransferase MTH_RS01935 MTH_RS07620  
trp PRAI: phosphoribosylanthranilate isomerase MTH_RS03970 MTH_RS07940  

Confidence: high confidence medium confidence low confidence
? – known gap: despite the lack of a good candidate for this step, this organism (or a related organism) performs the pathway

This GapMind analysis is from Apr 09 2024. The underlying query database was built on Apr 09 2024.



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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory