Alignments for a candidate for CGL in Clostridium acetobutylicum ATCC 824

GapMind for Amino acid biosynthesis

Alignments for a candidate for CGL in Clostridium acetobutylicum ATCC 824

Align cystathionine beta-synthase (O-acetyl-L-serine) (EC 2.5.1.134) (characterized)
to candidate WP_010963713.1 CA_RS02200 PLP-dependent aspartate aminotransferase family protein

Query= BRENDA::O05394
         (379 letters)



>NCBI__GCF_000008765.1:WP_010963713.1
          Length = 377

 Score =  400 bits (1029), Expect = e-116
 Identities = 192/377 (50%), Positives = 272/377 (72%)

Query: 1   MKKKTLMIHGGITGDEKTGAVSVPIYQVSTYKQPKAGQHTGYEYSRTANPTRTALEALVT 60
           MK  T +IH G  GD+ TGA+S PIYQ ST+ Q     H  Y+Y+R+ NPTR ALE  + 
Sbjct: 1   MKFGTKLIHSGFEGDKATGAISTPIYQTSTFHQENIDGHVTYDYARSGNPTREALENTIA 60

Query: 61  ELESGEAGYAFSSGMAAITAVMMLFNSGDHVVLTDDVYGGTYRVMTKVLNRLGIESTFVD 120
            LE G  G+AFSSGMAAI++V+ +F++ DH+++  DVYGGTYR+ T+  +R  +E  FVD
Sbjct: 61  LLEGGYKGFAFSSGMAAISSVLSIFSAHDHIIVPKDVYGGTYRITTQFFSRFEVECEFVD 120

Query: 121 TSSREEVEKAIRPNTKAIYIETPTNPLLKITDLTLMADIAKKAGVLLIVDNTFNTPYFQQ 180
           T+   EV++AI+ NTKAIYIETP+NPL+KITD+  + ++A++  +L+I DNTF +PY Q+
Sbjct: 121 TTDINEVQRAIKKNTKAIYIETPSNPLMKITDIRAIVELAREYKLLVIADNTFMSPYLQR 180

Query: 181 PLTLGADIVLHSATKYLGGHSDVVGGLVVTASKELGEELHFVQNSTGGVLGPQDSWLLMR 240
           PL LGADIV+HSATK+L GHSD+V GLVV  +KEL ++++FVQN+ G VL PQDSWLL+R
Sbjct: 181 PLELGADIVVHSATKFLNGHSDIVAGLVVAKNKELADKIYFVQNAFGAVLSPQDSWLLLR 240

Query: 241 GIKTLGLRMEAIDQNARKIASFLENHPAVQTLYYPGSSNHPGHELAKTQGAGFGGMISFD 300
           G+KTL +R+     NA ++A +L  +  V  ++YPG  +H G E+  +Q +G G ++SF+
Sbjct: 241 GMKTLKVRLNYQQNNATELAKWLLLNKFVDRVHYPGMPSHEGREIHLSQASGAGAVLSFE 300

Query: 301 IGSEERVDAFLGNLKLFTIAESLGAVESLISVPARMTHASIPRERRLELGITDGLIRISV 360
             S E+  +F+  +K   +  SLG VE+++S PA+M+HA IP   R++LGI D LIR+SV
Sbjct: 301 ACSTEKAKSFMKKVKCAAVGVSLGGVETIVSYPAKMSHAEIPHNERIKLGIGDNLIRVSV 360

Query: 361 GIEDAEDLLEDIGQALE 377
           G+ED EDL+E+   ALE
Sbjct: 361 GLEDIEDLIENFKIALE 377


Lambda     K      H
   0.316    0.134    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 395
Number of extensions: 11
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 379
Length of database: 377
Length adjustment: 30
Effective length of query: 349
Effective length of database: 347
Effective search space:   121103
Effective search space used:   121103
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis