Alignments for a candidate for metY in Clostridium acetobutylicum ATCC 824

GapMind for Amino acid biosynthesis

Alignments for a candidate for metY in Clostridium acetobutylicum ATCC 824

Align O-acetyl-L-homoserine sulfhydrylase; OAH sulfhydrylase; O-acetylhomoserine thiolase; EC 2.5.1.- (characterized)
to candidate WP_010966068.1 CA_RS14325 O-acetylhomoserine aminocarboxypropyltransferase/cysteine synthase

Query= SwissProt::Q9WZY4
         (430 letters)



>NCBI__GCF_000008765.1:WP_010966068.1
          Length = 427

 Score =  511 bits (1315), Expect = e-149
 Identities = 260/426 (61%), Positives = 327/426 (76%), Gaps = 5/426 (1%)

Query: 4   KKYGYNTRALHAGYEPPEQATGSRAVPIYQTTSYVFRDSDHAARLFALEEPGFIYTRIGN 63
           +K+G+ T  +HAG +  +  TGSRAVPIYQTTSYVF+++DHAA LF L+EPG +YTRI N
Sbjct: 5   RKFGFETLQVHAG-QVADPTTGSRAVPIYQTTSYVFKNADHAANLFQLKEPGNVYTRIMN 63

Query: 64  PTVSVLEERIAALEEGVGALAVASGQAAITYAILNIAGPGDEIVSGSALYGGTYNLFRHT 123
           PT  V E+R+AALE GV  LA ASG AAITYAILN+A  GDEIV+ S LYGGTY LF  T
Sbjct: 64  PTTDVFEQRVAALEGGVAGLATASGLAAITYAILNVASAGDEIVAASTLYGGTYELFGVT 123

Query: 124 LYKKSGIIVKFVDETDPKNIEEAITEKTKAVYLETIGNPGLTVPDFEAIAEIAHRHGVPL 183
           L KK GI V FVD  +P+NI +AI ++TKAVY ETIGNP + V D EA+A+IAH + +PL
Sbjct: 124 L-KKLGIKVVFVDPDNPENIRKAINDRTKAVYGETIGNPRINVLDIEAVAKIAHENKIPL 182

Query: 184 IVDNTVA-PYIFRPFEHGADIVVYSATKFIGGHGTSIGGLIVDSGKFDW-TNGKFPELVE 241
           I+DNT   PY+ RP E GADIVV+SATKFIGGHGT+IGG+IVD GKFDW  +GKFP+   
Sbjct: 183 IIDNTFGTPYLIRPIEFGADIVVHSATKFIGGHGTTIGGIIVDGGKFDWRASGKFPDFTT 242

Query: 242 PDPSYHGVSYVETFKEAAYIAKCRTQLLRDLGSCMSPFNAFLFILGLETLSLRMKKHCEN 301
           PD SY+G+ Y +     A+  K R QLLR+ G+ +SP +AF F+ GLE+LSLR++KH +N
Sbjct: 243 PDKSYNGLIYAD-LGAPAFALKARVQLLRNTGATLSPQSAFYFLQGLESLSLRVQKHVDN 301

Query: 302 ALKIVEFLKSHPAVSWVNYPIAEGNKTRENALKYLKEGYGAIVTFGVKGGKEAGKKFIDS 361
             K+VEFLK+HP VSW+NYP  E +  +E A KYL +G G+I TFG+KGG EAGK+FI+S
Sbjct: 302 TRKVVEFLKNHPKVSWINYPELEESPYKELANKYLPKGAGSIFTFGIKGGLEAGKRFINS 361

Query: 362 LTLISHLANIGDARTLAIHPASTTHQQLTEEEQLKTGVTPDMIRLSVGIEDVEDIIADLD 421
           + L S LAN+ DA++L IHP+STTH +L EEEQ   GVTPDMIRLS+G+ED ED+I DL+
Sbjct: 362 VKLFSLLANVADAKSLVIHPSSTTHAELNEEEQKAAGVTPDMIRLSIGVEDAEDLIWDLN 421

Query: 422 QALRKS 427
           QAL ++
Sbjct: 422 QALEQA 427


Lambda     K      H
   0.317    0.136    0.398 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 570
Number of extensions: 18
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 430
Length of database: 427
Length adjustment: 32
Effective length of query: 398
Effective length of database: 395
Effective search space:   157210
Effective search space used:   157210
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis