Alignments for a candidate for lysJ in Clostridium acetobutylicum ATCC 824

GapMind for Amino acid biosynthesis

Alignments for a candidate for lysJ in Clostridium acetobutylicum ATCC 824

Align [LysW]-aminoadipate semialdehyde/glutamate semialdehyde transaminase; EC 2.6.1.118; EC 2.6.1.124 (uncharacterized)
to candidate WP_010964671.1 CA_RS07165 adenosylmethionine--8-amino-7-oxononanoate transaminase

Query= curated2:Q7SI94
         (388 letters)



>NCBI__GCF_000008765.1:WP_010964671.1
          Length = 447

 Score =  178 bits (452), Expect = 2e-49
 Identities = 131/417 (31%), Positives = 206/417 (49%), Gaps = 49/417 (11%)

Query: 13  IKIIKGEGQYVWDEKNNKYLDMHAGHGVAFLGHRNKVIIDHLKKQMEEISTLSLA-FDTP 71
           I I KGEG Y++D   N YLD  +       GH NK I   ++KQ+ EI  +  A F   
Sbjct: 28  IVIDKGEGCYLYDVNGNSYLDCISSWWTNIFGHSNKRINKAIEKQLNEIEHVIFAHFSNK 87

Query: 72  IREEMIKELDELKPEDLDNLFLLNSGSEAVELALKIARKI------TKRRKIVAFKNSFH 125
              E  K L ++ PE LD +F  ++GS AVE+ALK++ +        K+ K  A  N++H
Sbjct: 88  PAVEFSKSLIQIAPEGLDKIFFSDNGSSAVEIALKMSFQYYQQTGKVKKTKFAALTNAYH 147

Query: 126 GRSMGALSVTWNKKYREPFEPLI-------GPVEF-LEYNNV-------------DSLKS 164
           G ++GALSV     Y + ++PL+       GP  +  +YN                SLK 
Sbjct: 148 GETIGALSVGDLDLYSKIYKPLLIDTLRIQGPDCYRCKYNKERHSCEAECFEEMEKSLKK 207

Query: 165 ITEDTAAVIVEP-VQGEGGVIPAKKEFVKSLREVTEKVNALLIIDEVQTGFGRTGKIWAY 223
              +  A+I+EP +QG  G+     +++K LR   +  +  +I DE+  GFGRTGK++A 
Sbjct: 208 HNSELCAIIIEPMIQGAAGMKMYSPKYLKKLRYACDYYDISVIFDEIAVGFGRTGKMFAC 267

Query: 224 QHFDIKPDILTAGKAIGGGF-PVSAVFLPNWISE-------KIEEGDHGSTYGGNPLAAA 275
           ++ DI PDI+   K I  G+ P+S V   + I          ++   H  TY GN LA A
Sbjct: 268 ENADISPDIMCLSKGISAGYMPMSVVLTTSKIYNAFYDDYVNLKAFIHSHTYAGNALACA 327

Query: 276 AVTAACKVAKSEKIAEQAQKKGELFMRILKEKLEDFKIVREIRGLGLMIGIDL------K 329
           A   + K+ K E I E+   K +L  ++ K +  + K + +IR LG++  I+L      K
Sbjct: 328 AAIESLKIFKEESIIEKNIGKSKLIEKLTKNRARNNKFIGDIRSLGMVTAIELVKDKDTK 387

Query: 330 VNPSIAIKVLQDEKVLSLKAGL------TTIRFLPPYLITQSDMEWASDATRKGISE 380
            +     +V  +   ++LK GL        + F+PPY I + D+E+  +   + I +
Sbjct: 388 ESFDWKSRVGYEIYKIALKKGLLLRPIGDVLYFMPPYSIGEKDIEFMVEKCFESIEQ 444


Lambda     K      H
   0.317    0.136    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 347
Number of extensions: 11
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 388
Length of database: 447
Length adjustment: 31
Effective length of query: 357
Effective length of database: 416
Effective search space:   148512
Effective search space used:   148512
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the original publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis