Align phosphoribosyl-AMP cyclohydrolase (EC 3.5.4.19) (characterized)
to candidate WP_012169472.1 AZC_RS04825 phosphoribosyl-AMP cyclohydrolase
Query= BRENDA::Q8YH95 (139 letters) >NCBI__GCF_000010525.1:WP_012169472.1 Length = 157 Score = 155 bits (391), Expect = 3e-43 Identities = 76/132 (57%), Positives = 93/132 (70%), Gaps = 5/132 (3%) Query: 6 AQPSDKKAVEEGAAFMPRFDASGLITAIVTDARDGELLMVAHMNEEALRLTLETGIAHYW 65 A+ +D +EEG PRFDA+GLIT + DA E+LM+AHMN EAL LT+ TG AHY+ Sbjct: 9 ARATDAHELEEGTVLAPRFDANGLITCVAVDAATNEILMLAHMNAEALSLTMATGEAHYY 68 Query: 66 SRSRKTLWKKGETSGNLQSVVELRTDCDQDALWLKVHVAGDGPTCHTGRRSCFYRQV--- 122 SRSRK LWKKGETSG+ Q V E+R DCDQDA+ +KV + G G CHTGRRSCFYR + Sbjct: 69 SRSRKALWKKGETSGHTQKVSEIRIDCDQDAVIIKVTMGGTGAACHTGRRSCFYRTIPLG 128 Query: 123 --VSSGGKVALT 132 S+ K+A T Sbjct: 129 APASADTKLAFT 140 Lambda K H 0.318 0.131 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 95 Number of extensions: 3 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 139 Length of database: 157 Length adjustment: 16 Effective length of query: 123 Effective length of database: 141 Effective search space: 17343 Effective search space used: 17343 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 42 (20.8 bits)
Align candidate WP_012169472.1 AZC_RS04825 (phosphoribosyl-AMP cyclohydrolase)
to HMM PF01502 (PRA-CH)
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.aa/PF01502.22.hmm # target sequence database: /tmp/gapView.3413107.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: PRA-CH [M=74] Accession: PF01502.22 Description: Phosphoribosyl-AMP cyclohydrolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.7e-39 118.8 0.5 4.9e-39 118.4 0.5 1.2 1 NCBI__GCF_000010525.1:WP_012169472.1 Domain annotation for each sequence (and alignments): >> NCBI__GCF_000010525.1:WP_012169472.1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 118.4 0.5 4.9e-39 4.9e-39 1 74 [] 47 122 .. 47 122 .. 0.96 Alignments for each domain: == domain 1 score: 118.4 bits; conditional E-value: 4.9e-39 PRA-CH 1 mlaymneealektletgkavyySrsrqklwkkGetsgnvqkvkeirldcDeDalllkveqk..gaaCHtgers 71 mla+mn+eal+ t++tg+a+yySrsr+ lwkkGetsg++qkv eir+dcD+Da+++kv+ gaaCHtg+rs NCBI__GCF_000010525.1:WP_012169472.1 47 MLAHMNAEALSLTMATGEAHYYSRSRKALWKKGETSGHTQKVSEIRIDCDQDAVIIKVTMGgtGAACHTGRRS 119 9**********************************************************883358******** PP PRA-CH 72 CFy 74 CFy NCBI__GCF_000010525.1:WP_012169472.1 120 CFY 122 **7 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (74 nodes) Target sequences: 1 (157 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 8.96 // [ok]
This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory