Alignments for a candidate for OAT in Azorhizobium caulinodans ORS 571

GapMind for Amino acid biosynthesis

Alignments for a candidate for OAT in Azorhizobium caulinodans ORS 571

Align Ornithine aminotransferase; Orn-AT; Lysine aminotransferase; Lys-AT; EC 2.6.1.13; EC 2.6.1.36 (characterized)
to candidate WP_012172510.1 AZC_RS20470 aminotransferase class III-fold pyridoxal phosphate-dependent enzyme

Query= SwissProt::Q5JEW1
         (445 letters)



>NCBI__GCF_000010525.1:WP_012172510.1
          Length = 475

 Score =  188 bits (478), Expect = 3e-52
 Identities = 137/427 (32%), Positives = 214/427 (50%), Gaps = 31/427 (7%)

Query: 37  PIVIERGEGIRVYDVDGNVFYDFASGVGVINVGHSHPRVVEAIKKQAEKFTHYSLTDFFY 96
           P + +R +G  +YD +   + D       +N G+ + R+ +A+ +Q ++    + + + +
Sbjct: 45  PKIFDRCQGSYMYDAEDVPYLDLQMWYSAVNFGYGNQRLNKALTRQLDRLPQVA-SQYLH 103

Query: 97  ENAIILAEKLIELAPGD--IERKVVYGNSGAEANEAAMKLVKYGT-GRKQFLAFYHAFHG 153
              I LA  + + A        +V +   G++A E A+KLV+    G+    AF   +HG
Sbjct: 104 AEKIELAATIAQHAEKTWGAGGRVHFNVGGSQAVEDALKLVRNARRGKGTMFAFEGGYHG 163

Query: 154 RTQAVLSLTAS-KWVQQDGFFPTMPGVTHIPYPNPYRNTWGIDGYEEPDELTNRVLDFIE 212
           RT    ++T+S ++ ++ G F        +P+P  +R   G+D  E       +     E
Sbjct: 164 RTLGASAITSSYRYRRRYGHFDR---ALFVPFPYHFRGPKGMDKEEYGLHCVKQFERLFE 220

Query: 213 EYVFRHVPPH----EIGAIFFEPIQGEGGYVVPPKGFFKALKKFADEYGILLADDEVQMG 268
                   P     E  A F EPIQG GGYV+PP  FF  LKK  D +GILL  DE+QMG
Sbjct: 221 SEYNGVWDPKAGEAECAAFFVEPIQGTGGYVIPPHNFFIELKKVLDRHGILLVVDEIQMG 280

Query: 269 IGRTGKFWAIEHFGVEPDLIQFGKAIGGGL-PLAGVIHRADITFDK---PGRHATTFGGN 324
           + RTGK W+IEHFGV PD++ FGKAI  GL PL+G+  R  +   +   PG   +TF  N
Sbjct: 281 VYRTGKLWSIEHFGVTPDVLVFGKAITNGLNPLSGIWARDALINPEVFPPGSTHSTFASN 340

Query: 325 PVAIAAGIEVVEIVKE---LLPHVQEVGDYLHKYLEEFKEKYEVIGDARGLGLAQAVEIV 381
           P+  A  +E + +V+E       V   G Y  + L+  +  ++V+GD  GLGLA  +EI 
Sbjct: 341 PLGTAVALETLRMVEEEQDFGAAVMAKGAYFLEGLQYLQRSHKVVGDVDGLGLALRMEIC 400

Query: 382 KSKETKEKYPELRDRIVK---------ESAKRGLVL--LGCGDNSIRFIPPLIVTKEEID 430
           + ++       L DR+V          +  + GLVL   G   N +   P L++TK EID
Sbjct: 401 E-EDGHTPNKALVDRMVDIALAADLELDGRRHGLVLDIGGYYKNVVTLAPSLLITKSEID 459

Query: 431 VAMEIFE 437
            A+ + +
Sbjct: 460 QAIRLLD 466


Lambda     K      H
   0.320    0.141    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 571
Number of extensions: 27
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 445
Length of database: 475
Length adjustment: 33
Effective length of query: 412
Effective length of database: 442
Effective search space:   182104
Effective search space used:   182104
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis