Alignments for a candidate for gatA in Pelobacter propionicus DSM 2379

GapMind for Amino acid biosynthesis

Alignments for a candidate for gatA in Pelobacter propionicus DSM 2379

Align Glutamyl-tRNA(Gln) amidotransferase subunit A; Glu-ADT subunit A; EC 6.3.5.7 (characterized)
to candidate WP_011736916.1 PPRO_RS15345 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA

Query= SwissProt::O06491
         (485 letters)



>NCBI__GCF_000015045.1:WP_011736916.1
          Length = 485

 Score =  525 bits (1351), Expect = e-153
 Identities = 261/486 (53%), Positives = 350/486 (72%), Gaps = 2/486 (0%)

Query: 1   MSLFDHKITELKQLIHKKEIKISDLVDESYKRIQAVDDKVQAFLALDEERARAYAKELDE 60
           M LFD  + EL   +  KE+   +       RI  ++ ++ AF+ +  ERA A A+  D 
Sbjct: 1   MDLFDLTLHELHAKLKSKEVSSREATSAMLDRIAELEPRINAFITVTPERALAEAEAADR 60

Query: 61  AVDGRSEHGLLFGMPIGVKDNIVTKGLRTTCSSKILENFDPIYDATVVQRLQDAEAVTIG 120
            +    E  +L G+P+ VKD  +T+G  TTC S+IL NF P Y AT  ++L+    V +G
Sbjct: 61  RI-AAGEADVLTGIPLAVKDIFLTEGTLTTCGSRILNNFIPPYSATSFEKLKQRGMVLLG 119

Query: 121 KLNMDEFAMGSSTENSAYKLTKNPWNLDTVPGGSSGGSAAAVAAGEVPFSLGSDTGGSIR 180
           KLN DEFAMGSS E+SA    +NPWN D +PGGSSGGSAAA+AA +   +LG+DTGGSIR
Sbjct: 120 KLNQDEFAMGSSNESSASGPCRNPWNTDCIPGGSSGGSAAAIAARQATVTLGTDTGGSIR 179

Query: 181 QPASFCGVVGLKPTYGRVSRYGLVAFASSLDQIGPITRTVEDNAFLLQAISGVDKMDSTS 240
           QPAS CG VGLKPTYGRVSRYG++A+ASSLDQ+GP+TR V D A +L A++G D  DSTS
Sbjct: 180 QPASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDCAIMLGALAGHDPKDSTS 239

Query: 241 ANVDVPDFLSSLTGDIKGLKIAVPKEYLGEGVGKEARESVLAALKVLEGLGATWEEVSLP 300
            +  VPD+ ++LT DI+GL+I +P+EY  EG+  + + S+  A++    LGA + E+SLP
Sbjct: 240 VDRPVPDYQAALTNDIRGLRIGLPREYFIEGLDPDVQASMDQAIETYRRLGAEFTEISLP 299

Query: 301 HSKYALATYYLLSSSEASANLARFDGIRYGYRTDNADNLIDLYKQTRAEGFGNEVKRRIM 360
           H+ YA+A+YYL++++EASANLAR+DG+R+G+R + A+ L+++Y ++R++GFG+EVKRRIM
Sbjct: 300 HTDYAVASYYLIATAEASANLARYDGVRFGHRAEQAEGLLEMYSRSRSQGFGSEVKRRIM 359

Query: 361 LGTFALSSGYYDAYYKKAQKVRTLIKKDFEDVFEKYDVIVGPTTPTPAFKIGENTKDPLT 420
           LGT+ALSSGYYDAYY KAQKVRTLI  DF   FE  D+I+ P  PTPAF+IGE   DPL 
Sbjct: 360 LGTYALSSGYYDAYYLKAQKVRTLIMADFIQAFEGVDLILTPVAPTPAFRIGEKVNDPLQ 419

Query: 421 MYANDILTIPVNLAGVPGISVPCGLA-DGLPLGLQIIGKHFDESTVYRVAHAFEQATDHH 479
           MY +DI TIPVNLAG   +S+P G++  GLP+G+Q+IGK F E T+ R AHAFEQAT+ H
Sbjct: 420 MYLSDIFTIPVNLAGTCAMSLPAGISGQGLPIGVQLIGKPFGEETILRAAHAFEQATEWH 479

Query: 480 KAKPEL 485
             +  L
Sbjct: 480 SLRAPL 485


Lambda     K      H
   0.315    0.134    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 602
Number of extensions: 21
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 485
Length of database: 485
Length adjustment: 34
Effective length of query: 451
Effective length of database: 451
Effective search space:   203401
Effective search space used:   203401
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

Align candidate WP_011736916.1 PPRO_RS15345 (Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA)
to HMM TIGR00132 (gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit (EC 6.3.5.-))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.aa/TIGR00132.hmm
# target sequence database:        /tmp/gapView.3604164.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00132  [M=466]
Accession:   TIGR00132
Description: gatA: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase, A subunit
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
   1.6e-193  629.6   0.0   1.9e-193  629.4   0.0    1.0  1  NCBI__GCF_000015045.1:WP_011736916.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000015045.1:WP_011736916.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  629.4   0.0  1.9e-193  1.9e-193       2     465 ..      12     477 ..      11     478 .. 0.99

  Alignments for each domain:
  == domain 1  score: 629.4 bits;  conditional E-value: 1.9e-193
                             TIGR00132   2 kellkkkevsikevleeilerieavkdkinaflevtkekalkkakkldkkva.ke.kklagipiavKdniavk 72 
                                           +++lk+kevs++e+++++l+ri + + +inaf++vt+e+al++a++ d+++a  e + l+gip+avKd + ++
  NCBI__GCF_000015045.1:WP_011736916.1  12 HAKLKSKEVSSREATSAMLDRIAELEPRINAFITVTPERALAEAEAADRRIAaGEaDVLTGIPLAVKDIFLTE 84 
                                           67899**********************************************986657**************** PP

                             TIGR00132  73 diettcaSkiLenyvspydatVverlkeagaliiGktNlDEFamGsstetSafgvtknPeneervpGGSsgGs 145
                                           ++ ttc+S+iL+n+++py+at +e+lk+ g++++Gk N DEFamGss e+Sa g+ +nP+n++ +pGGSsgGs
  NCBI__GCF_000015045.1:WP_011736916.1  85 GTLTTCGSRILNNFIPPYSATSFEKLKQRGMVLLGKLNQDEFAMGSSNESSASGPCRNPWNTDCIPGGSSGGS 157
                                           ************************************************************************* PP

                             TIGR00132 146 aaavaadlvplalgsDTGgSiRqPAsfcgvvGlKPtYGlvSRyGlvayasSldqiGilakkvedialvldvis 218
                                           aaa+aa ++ ++lg+DTGgSiRqPAs+cg+vGlKPtYG+vSRyG++ayasSldq+G+l+++v+d a++l +++
  NCBI__GCF_000015045.1:WP_011736916.1 158 AAAIAARQATVTLGTDTGGSIRQPASHCGCVGLKPTYGRVSRYGVIAYASSLDQVGPLTRDVTDCAIMLGALA 230
                                           ************************************************************************* PP

                             TIGR00132 219 gkDkkDstslevkveelleelkkdlkglkvgvvkelseesldkevkekfekllekleelgaeivevslpsvkl 291
                                           g+D kDsts++ +v+++  +l++d++gl++g+ +e++ e+ld +v++++++++e+ ++lgae++e+slp+ ++
  NCBI__GCF_000015045.1:WP_011736916.1 231 GHDPKDSTSVDRPVPDYQAALTNDIRGLRIGLPREYFIEGLDPDVQASMDQAIETYRRLGAEFTEISLPHTDY 303
                                           ************************************************************************* PP

                             TIGR00132 292 alaiYyiispsEassnlarydgiryGkrveelkslkelyaktRsegfgeevkrRimlGayalskeyydkyykk 364
                                           a+a Yy+i+++Eas+nlarydg+r+G+r+e+++ l e+y+++Rs+gfg+evkrRimlG+yals++yyd+yy+k
  NCBI__GCF_000015045.1:WP_011736916.1 304 AVASYYLIATAEASANLARYDGVRFGHRAEQAEGLLEMYSRSRSQGFGSEVKRRIMLGTYALSSGYYDAYYLK 376
                                           ************************************************************************* PP

                             TIGR00132 365 AqkvrtliidefeklfeevDvivsptaptlafklgekaedplemylsDvltvpanlaGlpaisvPlgkkekgl 437
                                           Aqkvrtli+ +f ++fe vD+i++p+apt af++gek +dpl+mylsD++t+p+nlaG++a+s+P+g + +gl
  NCBI__GCF_000015045.1:WP_011736916.1 377 AQKVRTLIMADFIQAFEGVDLILTPVAPTPAFRIGEKVNDPLQMYLSDIFTIPVNLAGTCAMSLPAGISGQGL 449
                                           ************************************************************************* PP

                             TIGR00132 438 piGlqiigkafddkkllsvakaleqald 465
                                           piG+q+igk f ++++l++a+a+eqa++
  NCBI__GCF_000015045.1:WP_011736916.1 450 PIGVQLIGKPFGEETILRAAHAFEQATE 477
                                           *************************986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (466 nodes)
Target sequences:                          1  (485 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 15.75
//
[ok]

This GapMind analysis is from Jul 25 2024. The underlying query database was built on Jul 25 2024.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer
changes to Amino acid biosynthesis since the publication.

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for Amino acid biosynthesis